Effect of some organic cosolvents on the reaction of hemoglobin with oxygen.
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Biomedical subjects
Publications and source records attributed to L Cordone.
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In the present work we studied the initial part of thermal denaturation curves of B. subtilis DNA in normal and heavy water, observing, by electron microscopy, the "opening" of DNA as a function of temperature. The results support the hypothesis that D(2)O plays a stabilizing role on strands separation during thermal denaturation of DNA.
Experimental results are reported showing the dependence of the U.V. induced enhancement of recombinants on the presence of the functional O gene product. This fact is tentatively interpreted as a replication dependence of the U.V. induced recombination.
The repair of U.V. damages to DNA in B. subtilis cultures competent for genetic transformation has been studied. The comparison of survival curves for competent and non competent fractions shows that: i) excision repair is more effective in competent than in non competent bacteria; ii) recombination repair is more effective in non competent than in competent bacteria. These facts support the hypothesis that metabolic conditions and, very likely, DNA replication play a role in the regulation of the efficiency of the two different mechanisms of repair.
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