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L F Marchenko

Publications and source records attributed to L F Marchenko.

48 records · Page 3Linked to original sources
PubMedDate not supplied

[Indices of the lipid spectrum of the blood serum and erythrocyte membranes in diabetes mellitus in children].

The paper is concerned with the results of a comparative study of the blood serum lipid spectrum and erythrocytic membranes in 100 children with diabetes mellitus aged 7 to 15 and 20 healthy children of the same age. The ratio of lipid and lipoprotein fractions depending on a degree of compensation, duration of disease and the presence of complications was disturbed in the blood serum and erythrocytic membranes of the diabetic children. The recovery of lipid metabolism was delayed as compared to clinical convalescence of the patients, therefore appropriate correcting therapy was required.

Adolescent↗
PubMedDate not supplied

[The cytogenetic examination of different groups of children living in regions of Bryansk Province contaminated as a result of the Chernobyl accident].

The frequency of chromosome aberrations (CA) was studied in peripheral blood lymphocytes from healthy children and children with thyreopathology living in the city of Klintsy, Bryansk Province (contamination level up to 5 Ci/km2), and two Moscow groups, respectively. We have observed the elevated number of dicentrics and acentric fragments in cell from children with thyreopathology with respect to those from healthy children living in Klintsy. This fact cannot be explained by influence of disease because there was no difference in cytogenetical markers between the groups of healthy children and children with thyreopathology from Moscow. The number of dicentrics was increased in cells from children with high level of internal contamination (more than 400 nCi) living in Krasnaya Gora (15-40 Ci/km2). It is proposed that the internal irradiation is more important for the CA induction. The symmetrical translocation analysis using the method of fluorescence in situ hybridisation (FISH) has shown the absence of this type of aberrations in cells from children with high level of internal irradiation whereas the translocations frequency in cells from control children was (1.1 +/- 0.4) per 1000 cells.

Adolescent↗
PubMedDate not supplied

[The frequency of chromosome aberrations in the lymphocytes of children with thyroid diseases living in Bryansk Province].

The number of unstable chromosome aberrations was determined in peripheral blood lymphocytes from 15 children with thyreopathology living in Klintsy (Bryansk Province); the mean age was 14 years. The number of dicentric chromosome is determined to be (0.18 +/- 0.07) per 100 cells and is significantly higher than that in cells from healthy children of Klintsy and Moscow (control). There was no differences in the frequency of other types of chromosome aberrations as well as of aberrant cells.

Adolescent↗
PubMedDate not supplied

[Use of electron paramagnetic resonance in assessing viscosity of blood lipids in diabetes mellitus in children].

Viscosity of blood serum lipids was studied in children with insulin-dependent diabetes involving nitroxyl containing probes-registered by means of radiospectrometer RE 1307. The following parameters were studied: the ratios between values exhibiting the probe distribution in polar and hydrophobic phases [formula: see text] as well as between content of low polar (I+1) and high polar (I-1) spectral components. The "f' value showed the state of lipid density and the ratio [formula: see text] allowed to estimate the lipid microviscosity. 34 children of 7-15 years old with insulin-dependent diabetes were examined in dynamics of treatment under stationary conditions. Distinct metabolic impairments and aggravation of the lipid peroxidation syndrome were mainly responsible for pronounced increase in lipid viscosity. The procedure of spin probes may contribute to study of diabetes pathogenesis as well as to serve as a diagnostic means in evaluation of the disease complications at early stages.

Adolescent↗
PubMedDate not supplied

[The determination of microalbuminuria by immunoenzyme analysis].

The authors have defined the optimal conditions for inhibitory type enzyme immunoassay of microalbuminuria. The range of albumin detection is 30 to 250 micrograms/ml. The optimal time of antigen binding with antibody is 45 min, the lower threshold level of albumin detection 0.6 +/- 0.02 microgram/ml. Albumin concentration is unchanged after urine storage for 8 weeks at -20 degrees C or for 1 week at 4 degrees C.

Albuminuria↗
PubMedDate not supplied

[Distribution of the total quantity of fatty acid double bonds in lipid components of the blood in children with diabetes mellitus].

Children suffering from insulin-dependent diabetes mellitus (IDDM) were examined for unsaturation, that is, total quantity of double bonds in individual fractions of blood serum lipids was assessed. Lipid fractions were isolated by thin-layer chromatography. Unsaturation was assessed by ozonation method. The research was aimed at elucidation of biochemical criteria of the degree of metabolic disorders in children with IDDM and at development of methods for quantitative assessment of such disorders. Thirty-two inpatients with IDDM aged 7 to 15 were examined over time. Control group included 10 healthy age-matched children. Twenty children were examined during compensation stage (group 1), twelve during decompensation with ketoacidosis (group 2). Total unsaturation parameters were found reliably decreased in children with IDDM both during compensation and decompensation stages vs. those in healthy controls. A relative decrease of unsaturated fatty acids in the fraction of unesterified fatty acids (p < 0.05) was characteristic of group 2, with the content of their fragments in triglyceride fraction being the maximal (p < 0.05). The pattern of total unsaturation distribution in serum lipid fractions may serve as an indicator for metabolic disorders and decompensation in IDDM.

Adolescent↗
PubMedDate not supplied

[An evaluation of the radioprotective action of antioxidants on normal human cells and in genomic diseases by using different cytogenetic tests].

In studying the radioprotective action of natural and synthesised antioxydants a decreased yield of chromosome aberrations with respect to those in untreated cells was noted in normal cells irradiated in phase G1 whereas no radioprotective effect was found in cells irradiated in G0. The addition of antioxydants into the cell cultures from patients with Turner's syndrome did not change their radiosensitivity. No adaptive response was induced in lymphocytes from patients with Down's syndrome cultivated with vitamin E.

Adaptation, Physiological↗
PubMedDate not supplied

[Microcirculatory hemostasis in children with insulin-dependent diabetes mellitus].

Study of microcirculatory hemostasis in children with insulin-dependent diabetes mellitus helped the authors to make new conclusions on metabolic changes underlying blood coagulation cellular factors, namely, the red cell and platelet levels. Fifty children aged 7 to 15, suffering from type I condition, were examined. Ten of these were examined during the compensation stage, 23 during decompensation without ketosis, and 17 with ketoacidosis. Twelve age-matched healthy children were controls. Noticeable disorders in the microcirculatory hemostasis system were revealed in diabetic children, these changes manifesting by changed functional activities of red cells and platelets. Reduced share of platelet deaggregation, increased red cell aggregation time, reduced thromboelastogram chronometric constant and reduction of its structural parameters may be considered as the early signs of these changes. The risk of microcirculatory hemostasis disturbances augments with the disease duration and progress.

Adolescent↗