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Biomedical subjects

L F Poriadkov

Publications and source records attributed to L F Poriadkov.

At least 19 recordsLinked to original sources
PubMed2003

[Effect of enterally administrated oxygenated water on electrical activity of the small intestine in early postoperative period in rats].

The effect of oxygen, structurally-binded with water (oxygen water), on electrical activity of rat jejunum in early postoperative period. It was shown, that in contrast to effect of 0.9% NaCl, there is normalization of electrical activity parameters (frequency and slow wave amplitude) of jejunum in early postoperative period under the influence of oxygen water.

Action Potentials↗
PubMed1999

[Utilization of glucose, valine, glycerin and palmitic acid by energy and anabolic pathways of metabolism in rats].

Possibility of evaluation of nutrients use by energy and anabolic ways by measurement in blood of radioactivity of water formed from labelled by tritium precursors was studied. The experiment was made on rats, which consecutively with interval 1.5-2 weeks were given intravenous indicative amount of 3H2(2,3)-valine, 1-14C-glucose, 6-3H-glucose, 3H2(1,3)-glycerin and 3H2(9,10)-palmitic acid. Half-time of glucose in blood was 46 min, glycerin--16 min, valine and palmitic acid--24 min. Plastic and energy characteristics of these substances were studied. The proposed approach to the evaluation of plastic and energy functions of nutrients can be useful for development of specialized products for enteral and parenteral feeding.

Animals↗
PubMed1983

[Participation of the gastrointestinal tract in regulating the processes of insulin liberation by the pancreas].

Three elevations of the insulin concentration in the blood of the v. pancreaticoduodenalis and v. portae were found in dogs fed with food containing mainly proteins, fat or carbohydrates. The gastrointestinal tract was found to potentiate the liberation of insulin. The elevation of the latter concentration in the blood occurred before the intensive absorption of the nutrients aided to, apparently, by liberation of some gastrointestinal hormones (gastrin, cholecystokinin-pancreozymin, GIP and others) which prepare the tissues for the assimilation of nutrients. A later elevation of the insulin concentration in the blood is possibly connected with absorption of aminoacids and some other nutrients and/or liberation of GIP in the small intestine.

Animal Nutritional Physiological Phenomena↗
PubMedDate not supplied

[Hydrolytic splitting of vitamin A esters in the blood].

The retinol and retinylpalmitate content along with the retinylacetate and retinylpalmitate-hydrolase in the blood of the portal vein and in the peripheral blood from the superficial vein of the hind limb of dogs was determined in chronic experiments before and 1, 2, 3 and 4 hours after administration of the vitamin A preparations into the small intestine fistula. The introduction of an oily retinylacetate solution into the fistula was attended both by an increase of the retinylpalmitate content in the blood plasma and the appearance therein of the retinyl-palmitat-hydrolase activity. Introduction of an aquous-colloidal solution of retinyl-palmitate caused a rise in the latter's content in the blood but was not accompanied by the appearance of the retinyl-palmitate-hydrolase activity. In the blood taken on an empty stomach no capacity for retinyl-palmitate hydrolysis was in evidence. It is presumed that the emergence of the retinyl-palmitate activity in the blood following introduction of precisely the oily retinylacetate solution is due to an intensified supply of the pancreatic enzymes into the blood in response to the fat load. The retinylacetate-hydrolase activity was continually present in the blood and did not vary in its intensity in the dynamics of absorption.

Animals↗
PubMedDate not supplied

[The methodological problems of an experimental study of artificial feeding].

General requirements have been presented for the organization and performing of experiments on artificial feeding in small laboratory animals. Some operation methods and devices applicable in the investigation of the body functions under conditions of parenteral and tube feeding have been described. The possibility of using this experimental biological model in the study of artificial feeding has been evidenced by the results of the test on 15-day tube feeding of rats.

Animals↗
PubMedDate not supplied

[Proteolytic activity of lysosomes in various rat organs during total parenteral nutrition].

Specific, total and nonsedimented activities of cathepsins A, B, C and D as well as of aryl sulfatases A and B, beta-galactosidase, beta-glucuronidase and N-acetyl-beta-D-glucosaminidase were studied in liver, kidney and spleen tissues of rats under conditions of artificial intragastric and parenteral nutrition. Activity of cathepsins A and D in liver tissue as well as total activity of all the lysosomal hydrolases studied in spleen was distinctly increased in parenteral nutrition. These data suggest elevation in the functional activity of lysosomal apparatus under conditions of parenteral nutrition.

Animals↗
PubMedDate not supplied

[Various features of the initial stages of assimilation of isotope-labelled dipeptide].

Tritium-labeled dipeptide Gly-Leu was administered to noninbred guinea pigs by intracardiac injections, and to Wistar rats--intra-intestinally, through a specially implanted catheter. It was found that after intravenous and intraintestinal administration of isotope-labeled dipeptide the "low-molecular label" was bound by serum proteins. Dissociation of the complexes "low-molecular label"--serum protein in 8 M solution of urea has evidenced a predominantly reversible character of interaction between the low-molecular ligand and protein macromolecule. The results obtained should be taken into consideration during assay of protein absorption from gastro-intestinal tract of laboratory animals.

Animals↗
PubMedDate not supplied

[Digestive function of the small intestine and pancreas in rats on artificial feeding].

Full-value diets of similar composition were given to male rats weighing 207-230 g, by intravenous (group 1) or intragastric (group 2) routes. The proportion of amino acids, fats and carbohydrates was 9.9:15.7:74.4 (with regard to their calorific value). The diet calorific value comprised 60.6 kcal/rat/day. An average mass increase in group 1 was 2.44 +/- 0.14 g/day, in group 2 - 1.75 +/- 0.11 g/day. The protein content and activities of alpha- and gamma-amylase, invertase, maltase, and glycil-L-leucine dipeptidase were assayed in the intestinal mucosa of the proximal portion of the small intestine in group 1 rats, while a decreased alpha-amylase activity in the distal portion of the small intestine was recorded in the animals of group 2. The mass of the pancreas in the rats of group 1 and 2 was authentically lower than in the control rats which received oral feeding with natural foods. The lowest mass of the pancreas was observed in the rats of group 1. Specific activity of trypsin, lipase and RNase in the pancreatic tissues of rats in groups 1 and 2 was similar. The results of the study have evidenced a lowered function of the digestive system under conditions of artificial feeding, especially in case of intravenous nutrition.

Animals↗
PubMedDate not supplied

[Content of mineral substances in the internal organs of rats on intravenous feeding].

The experiments on rats receiving a complete intravenous nutrition (group 1) during 15 days showed that the concentration of natrium, magnesium and iron was increased in the liver and decreased in the spleen as compared to that in controls given normal feeding (group 2). The content of zink and copper (mg%) was significantly lowered in both the organs of rats in group 1. The growth of the liver and spleen mass during parenteral feeding led to a significant rise in the content of natrium, potassium, magnesium, iron, zink and manganese in the whole liver tissue of group 1 rats, while the content of calcium and copper was similar in both groups of animals. The content of natrium, potassium, magnesium, iron and zink in the whole spleen tissue was significantly higher in group 1 rats, while the content of calcium, copper and manganese was similar in both groups of rats. A correlation was revealed between the content of iron and copper in the liver tissue (r = +0.87) and the levels of natrium and magnesium it the spleen tissue (r = +0.78) of group 1 rats. In group 2 rats a correlation was recorded between the content of calcium and manganese in the liver tissue an the levels of natrium and calcium in the spleen (r = +0.85 and +0.86, respectively).

Animals↗
PubMedDate not supplied

[Absorption and the immune response to ovalbumin administered by various methods in the dogs].

Absorption and immune response to ovalbumin (OA) were studied in chronic experiments on dogs after intraenteral administration (5 dogs) and feeding (3 dogs) with non-boiled hen eggs. A modified concurrent radioimmunoassay was used to estimate the blood serum content of OA antigenic structures and antibodies to them. In 7 out of 8 dogs the antigen was detected in blood already after the first intraenteral administration or feeding. It was established that absorption of OA antigenic structures was more intensive after intraenteral administration than after feeding with hen eggs. Antibodies to OA were detected in blood on day 15 in case of intraenteral administration and on day 20 when the animals were fed with eggs. The blood antibody titre to OA in dogs receiving ovalbumin by intraenteral route was 10-fold higher as compared to that in dogs fed with hen eggs. The data obtained evidence a high potential risk of the body sensitization to food protein during intraenteral food administration, as it happens, for example, in tube intraenteral feeding.

Administration, Oral↗
PubMedDate not supplied

[Effects of parenterally administered dicarboxylic amino acids and their amides on the secretory function of the stomach].

The effect of dicarboxylic amino acids and their amides on gastric secretion stimulated by sham-feeding (100 g of raw meat), as well as of amides of dicarboxylic amino acids on the gastric secretory function was studied in 119 tests on 8 dogs with gastric fistulas according to Basov. The tests were started 16-18 h after feeding under neutral or alkaline reactions of gastric excretion. The tests demonstrated that dicarboxylic amino acids inhibited gastric secretion induced by sham-feeding. It was shown that dicarboxylic amino acid amines, in contrast to these amino acids, did not inhibit gastric secretion induced by sham-feeding. On the contrary, asparagine and glutamine administered into the blood are capable of inducing gastric secretion, per se.

Alanine↗