PubMed Health⌕ Search

Biomedical subjects

L Franke

Publications and source records attributed to L Franke.

At least 37 records · Page 2Linked to original sources
PubMed1990

[The cytochemical detection of monocytes, lymphocytes, neutrophilic and eosinophilic granulocytes by using marker enzymes].

Some cytochemical methods for determination of monocytes, neutrophilic and eosinophilic granulocytes were modified on the basis of manifestation of the respective marker enzymes (alpha-naphthyl-acetatesterase, naphthol-AS-D-chloracetatesterase and cyano-resistant peroxidase). The modification presented formation of economic methods by using some common reagents for identification of single esterases and peroxidases.

Biomarkers↗
PubMed1989

[An in vitro micromethod for studying phagocytic and bacteriocidal activity of granulocytes from peripheral blood].

A microcultivation method convenient for clinical objectives was prepared for simultaneous examination of phagocytic and bactericidal capability of human granulocytes, based on vital staining with acridine-orange. Slides, on which nonheparinized blood from the examined individuals was poured out drop by drop, were assembled on the microcultivation camera, constructed by us. After one hour incubation the clot was removed and a suspension from Staphylococcus aureus was poured out on the cellular film. After proper cultivation the adherent cells were stained with acridine-orange as the live bacteria and nuclei of intact granulocytes were stained green under the action of the dye, but the dead prokaryotes and degenerated nuclei-red. The phagocytic index, phagocytic number, bacteriocidal index were followed-up in dynamics and it was established that a 45-minute incubation appeared to be the most suitable for simultaneous examination of these parameters.

Blood Bactericidal Activity↗
PubMed1987

Radioiododestannylation: preparation and evaluation of radioiodinated thienyl alcohols.

Radioiododestannylations was employed to prepare a series of four specifically labeled thienyl alcohols: 1-(5-iodo-2-thienyl)-cyclopentan-1-ol and -cyclohexan-1-ol; 17 alpha-(5-iodo-2-thienyl)-17 beta-estradiol and -estradiol-3-O-methyl ether. The method utilized 5-(trimethylstannyl)thienyl intermediates which had been prepared in good yields from 2,5-bis(trimethylstannyl)thiophene and the appropriate cyclic ketones. The trimethylstannyl substrates reacted with no-carrier-added Na125I in the presence of 30% H2O2-acetic acid (2:1) at pH 4.5 at ambient temperature for 5-15 min to give, after HPLC separation, the desired 5-[125I]iodothien-2-yl products in greater than 90% isolated yields. Although tissue distribution studies in rats were uneventful, the methodology employed to give the labeled products possesses distinct advantages compared to alternative methods for preparing radioiodinated aryl moieties.

Animals↗
PubMed1987

[Changes in the gangliosides of erythrocyte membranes of schizophrenic patients].

The pattern of gangliosides in membranes of erythrocytes was examined in healthy donors, in acute schizophrenics without neuroleptic treatment and in alcohol-dependent patients. 7 different gangliosides could be detected after extraction, purification by column chromatography and fractionation by TLC. Healthy donors were characterized by the following pattern of gangliosides: GX = 5.8%, GT1b = 6.7%, FucGD1b = 5.2%, und GD1a = 12.6%, GD3 = 9.2%, SPG = 43.5% and GM3 = 17.0%. In schizophrenic patients the GM3- and GD3-fraction were increased. No difference was found between the control group and the alcoholics.

Adolescent↗
PubMed1985

Preparation and evaluation of 3-[125I]iodo-4-aminophentermine as a brain perfusion imaging agent. Comparison with labeled phentermine derivatives.

A radioiodinated derivative of the anorexinergic drug phentermine was synthesized and evaluated as a potential brain imaging agent. Stoichiometric iodination of the intermediate 4-aminophentermine (AmP) gave a mixture of mono and diiodo products, while the radioiodination at the no-carrier-added (NCA) level gave 73% isolated yield of the 3-[125I]iodo-4-aminophentermine with less than 2% of the diiodo derivative. The tissue distribution of the radiochemical in rats showed that it was readily extracted by the brain followed by relatively slow clearance from that organ. However, a comparison with other labeled phentermine derivatives indicated that the total brain uptake, retention and selectivity of the new agent were poorer, probably as a result of the presence of the 4-amino substituent.

Animals↗
PubMed1984

[beta-Phenylethylamine in the cisternal fluid in acute endogenous psychoses].

Beta-Phenylethylamine can be detected in the cisternal cerebrospinal fluid of healthy controls and acute endogenous psychoses. One group of the patients shows the same concentrations as the psychiatrically healthy controls. In the other groups beta-phenylethylamine was found to be elevated in some cases up to a very high level. The results suggest a link between a disturbance of the phenylethylamine pathway and certain endogenous psychoses.

Humans↗
PubMed1983

[Methylated and unmethylated indolamine in the cisternal fluid in acute endogenous psychoses].

Tryptamine (Try), 5-methoxytryptamine (5MeOT), N-methyltryptamine (N-Met), 5-methoxy-N,N-dimethyltryptamine (5MeODMT) and N,N-dimethyltryptamine (DMT) are metabolites of the neurotransmitter 5-hydroxytryptamine (5HT). The cisternal cerebrospinal fluid (CSF) is probably a suitable biological material for investigating the problem of indolamine metabolism in schizophrenic patients. As part of a biological research programme on schizophrenia we have investigated concentrations of cisternal CSF indolamines in a group of acute paranoid patients in comparison with psychiatrically healthy controls. The concentrations of indolamines in the cisternal CSF reveal that psychotomimetic indolamines like 5MeODMT, 5MeOT and DMT and the indolamines N-Met and Try are present in psychological and pathological states. The patients suffering from acute paranoid schizophrenia have high or very high levels of the investigated indolamines in comparison with healthy controls, but there are significant individual differences in the group of these patients. The search for correlations between the level of a single indolamine and individual psychopathological symptoms could provide more specific information for diagnosis or treatment.

Acute Disease↗
PubMed1980

[Extraction of serotonin, bufotenin, 5-methoxytryptamine and 5-methoxy-N,N-dimethyltryptamine and their fluorometric determination with o-phthaldialdehyde].

For the investigated indolamines, the recovery following extraction with n-butanol and acetic acid ethyl ester from alkaline solution amounted to 80-90%. Using benzene only the two 5-methoxyindolamines were transferred from the alkaline medium into the organic phase. The reaction of the o-phthaldialdehyde with substituted indolamines was optimized in regard to higher fluorimetric detection sensitivity. The lower limit of determination was 2.0 ng for 5HT and 5OHDMT, and 1.0 ng for 5MeOT and 5MeODMT.

5-Methoxytryptamine↗