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Biomedical subjects

L Fumagalli

Publications and source records attributed to L Fumagalli.

At least 91 records · Page 5Linked to original sources
PubMed1988

Incidence of jaw lesions in 193 patients with multiple myeloma.

The incidence of alterations of the maxilla and mandible at radiographic screening was evaluated in 193 patients with multiple myeloma, both at the time of diagnosis and after the start of chemotherapy during the course of the disease. Although the study confirms that osteolytic lesions are more frequent in the mandible than in the maxilla, it showed that their incidence was lower than reported in the past. Furthermore, these lesions were mostly asymptomatic and were observed in patients who had extensive skeletal involvement, particularly at the skull level. These findings indicate that systematic radiologic monitoring of the stomatognathic system should be performed, particularly in patients with multiple myeloma at an advanced stage.

Adult↗
PubMed1987

Vascular localization of bovine pancreatic trypsin-inhibitor-related molecular forms in bovine spleen.

Bovine spleen proteic inhibitors of serine proteases, belonging to the bovine pancreatic trypsin inhibitor (BPTI or aprotinin) family, have been localized, using immunocytochemical techniques, in the smooth muscle cells of some bovine spleen blood vessels. This vascular localization also occurs in a variety of bovine organs and differs from that of BPTI itself which is found exclusively in bovine mast cells, in agreement with previous reports. These data would be in favour of a possible involvement of one or more BPTI-type inhibitors in vascular processes by acting at the level of the smooth muscle cells, the tissue responsible for vasodilation/vasoconstriction events.

Animals↗
PubMed1987

Ultracytochemical detection of heteropolysaccharides in the cat submandibular gland.

We have investigated the morphology and the ultrastructural distribution of complex carbohydrates in the cat submandibular gland using the high iron diamine-thiocarbohydrazide-silver proteinate (HID-TCH-SP), the low iron diamine-thiocarbohydrazide-silver proteinate (LID-TCH-SP), and the periodate-thiocarbohydrazide-silver proteinate (PA-TCH-SP) sequences. The results of morphological analyses show that, in addition to already known cellular populations, in striated ducts a further cellular type containing glycogen is present. The histochemical staining showed that demilunar cells secrete polysaccharides that have carboxylated but not sulphated glycosidic residues, whereas acinar cells elaborate secretion products rich in carboxylated and sulphate-containing complex carbohydrates. Vicinal glycol groups are present as constituents of the secretion in demilunar, acinar, and ductal cells.

Animals↗
PubMed1986

Light and electron microscopic study of cyclosporin A-induced gingival hyperplasia.

Four transplant (3 kidney, 1 bone marrow) patients with cyclosporin A (CyA)-induced gingival hyperplasia are described. Light and electron microscopic findings of gingival biopsies showed in all patients that, in addition to an increase of collagen, CyA induced in the subepithelial space an enormous infiltration of morphologically normal plasma cells in different stages of maturation. These data, together with the reversibility of the lesion upon discontinuation of the drug, suggest that individual hypersensitivity is probably the most acceptable explanation of CyA-induced gingival hyperplasia. This hypothesis is also discussed in relation to the CyA-suppression of the functions of some T-lymphocyte subsets.

Adult↗
PubMed1985

Characterisation in situ of the complex carbohydrates in rabbit oviduct using digestion with glycosidases followed by lectin binding.

Enzymatic degradation followed by lectin application was carried out on specimens of the rabbit oviduct. The sequential application of the glycosidases (neuraminidase, alpha-L-fucosidase, beta-galactosidase and alpha-mannosidase) and lectins (peanut, winged pea, wheat germ and soybean) seem promising as an effective method for characterising, in situ, the structure of complex carbohydrate chains. The data obtained after digestion with neuraminidase and alpha-L-fucosidase indicate the presence of a continuous layer of sialoglycoconjugates and fucoglycoconjugates that completely covers the luminal surfaces of the ampulla. The different reactivity of the glycocalyx and the secretory products indicates a different chemical composition of glycoconjugates in the glycocalyx and secretions. Although it is very difficult to explain the lectin affinity and the functional significance of the positive cells, some hypotheses have been advanced.

Animals↗
PubMed1983

[Sampling of tissue from microscopic areas of the spinal cord of the chick embryo. Description of the technic and application to the study of enzymes metabolizing acetylcholine].

In the present communication we describe a very simple procedure which allows measuring the specific activity of cholinergic enzymes (Acetylcholinesterase and cholinacetyltransferase) in microscopical areas of chick embryo spinal cord. Microhomogenates were prepared as follows: i) a number of fresh cryostat sections (20 mu thick) were stained with toluidine blue; ii) the adjacent unstained sections were microdissected under stereomicroscope and tissue fragments were collected from different areas (lateral, medial and posterior columns; anterior, lateral and posterior bundles). Tissue fragments were dissolved in 10 mM sodium-phosphate buffer pH 7.4 (20-40 sections). Protein assay was performed according to Bradford (1976). AChE and ChAc were measured by radiochemical methods of Fonnum (1969, 1970). In lumbar segments of 18-day chick embryo spinal cord AChE was found in all structure investigated, by contrast ChAc was localized almost exclusively in lateral somatomotor comumns. This result indicates that ChAc can be considered a specific marker for motoneurons. The ontogeny of the cholinergic enzymes in various areas of the chick embryo spinal cord has been investigated.

Acetylcholine↗
PubMed1980

[Autoradiographic localization of acetylcholine receptors in the rat superior cervical ganglion].

alpha-Bungarotoxin-acetylcholine receptor localization has been studied by means of light and electron microscope autoradiography. The present results suggest that the receptors are located in the neuropil (probably on dendrites) and in the neuronal cytoplasm. The silver grains do not appear localized at postsynaptic level. According to previous electrophysiological data, the present results suggest that in the rat superior cervical ganglion the alpha-bungarotoxin-acetylcholine receptors are not involved in synapticc transmission.

Acetylcholine↗
PubMed1980

[Development of some cholinergic components in rat sympathetic ganglion].

Postnatal development of some cholinergic components has been studied in the rat superior cervical ganglion. Choline-acetyltransferase, the enzyme localized exclusively at the preganglionic nerve terminals, follows the same developmental pattern of the postganglionic alpha-bungarotoxin-acetylcholine receptors. These results suggest that pre- and postganglionic structures develop simultaneously and parallel. The ontogeny of acetylcholinesterase, a cholinergic enzyme localized at both pre- and postganglionic level, has been also investigated.

Acetylcholinesterase↗
PubMed1980

[Effect of preganglionic activation on nicotinic cholinergic receptors linked to alpha-bungarotoxin in the rat superior cervical ganglion].

alpha-Bungarotoxin binds to nicotinic cholinergic sites in the rat superior cervical ganglion. In physiological conditions the receptors are relatively independent upon preganglionic signals, as suggested by experiments of preganglionic denervation. In the present paper we show that the receptors are also uneffected by the afferent nerve fibres when preganglionic activation is induced by Reserpine administration or by cold exposure.

Animals↗
PubMed1980

[Acetylcholine receptors and acetylcholinesterase activity in cultured chick ciliary ganglia].

Acetylcholine receptor number and the acetylcholinesterase activity have been studied in organ-cultured chick ciliary ganglia. Receptor number decreases progressively reaching minimum value after two days of culture. Acetylcholinesterase activity decreases slowly reaching at day 3 the 75% of the original value. The biological significance of these data is discussed in relation to the ganglion disconnection from the periphery and from the central nervous system.

Acetylcholinesterase↗
PubMed1979

Appearance of new alpha-bungarotoxin-acetylcholine receptors in cultured sympathetic ganglia of chick embryos.

alpha-Bungarotoxin (alpha-BuTX) has been used as a marker for studying the production of alpha-bungarotoxin-acetylcholine receptors (alpha-BuTX-AChRs) in explants of chick embryo sympathetic ganglia cultured in vitro. New alpha-BuTX-AChRs appear rapidly in the explants after blocking of the pre-existent ones with the toxin (40% of the total receptors at 3 h). There is a portion of alpha-BuTX-AChRs in the explants which for a short time is not accessible to the toxin. This portion constitutes the precursor pool of receptors and represents 18% of the total. The precursor pool of receptors supplies the neurons with new receptors for 1-2 h in the absence of protein synthesis. The appearance of new receptors from the precursor pool is an energy-dependent process.

Acetylcholine↗
PubMed1978

alpha-Bungarotoxin-acetylcholine receptors in the chick ciliary ganglion: effects of deafferentation and axotomy.

alpha-Bungarotoxin (alpha-BuTX) binds in a saturable and practically irreversible fashion to membrane-associated receptors in the ciliary ganglion of the adult chick. The binding of toxin to receptors is competitively inhibited by nicotinic cholinergic ligands, and for these properties the receptors are regarded as acetylcholine receptors of the nicotinic type (alpha-buTX-AChRs). The rate constant of association (K1) and dissociation (K-1) of the toxin-receptor reaction has been estimated to be K1 = 7.4 x 104 M(-1) sec(-1) and K-1 = 9.6 X 10(-6) sec(-1), respectively. Light autoradiography shows that most, if not all, the receptors are related to surface membrane, probably to synaptic areas of both choroid and ciliary neurons. The choroid neurons contain more receptors than the ciliary ones. A single chick ciliary ganglion binds specifically 47 fmole of alpha-BuTX in situ corresponding to 2.83 x 1010 alpha-BuTX-AChRs/ganglion. No changes in number and distribution of the toxin receptors occur following preganglionic denervation. Conversely, postganglionic axotomy causes a rapid disappearance of the receptors in situ. Since binding experiments in vitro revealed a partial, instead of a total, loss of the receptors, it is suggested that the disappearance of the receptors in situ includes both a partial loss of the original receptors and the masking of the residual ones.

Acetylcholine↗