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Biomedical subjects

L Guida

Publications and source records attributed to L Guida.

At least 109 records · Page 6Linked to original sources
PubMedDate not supplied

A retrospective study of 77 cats with severe hepatic lipidosis: 1975-1990.

The physical, clinicopathologic, and survival rates of 77 cats with severe spontaneous hepatic lipidosis are detailed in this report. Cats were subdivided into groups designated as idiopathic lipidosis if no other disease process was recognized, or secondary lipidosis if another disease process was diagnosed. Cats were also subdivided into groups designated as survivors or nonsurvivors on the basis of successful recuperation at 4 months after initial diagnosis. Differences between disease and survival groups were evaluated for significance. Overall, more female cats and middle-aged cats were affected. Presenting complaints of vomiting, anorexia, weakness, and weight loss were common. Physical assessment of most cats showed obvious hepatomegaly, jaundice, dehydration, and a weight loss > or = 25% of usual body weight. Neurobehavioral signs indicative of hepatic encephalopathy, other than ptyalism and depression, were rare. Clinicopathologic features are characterized by hyperbilirubinemia and increased activities of serum ALT, AST, and ALP, with only small if any increase in gamma GT activity. Clinical features distinguishing cats with hepatic lipidosis from those with other serious cholestatic disorders include absence of hyperglobulinemia and low gamma GT activity relative to ALP activity. Although coagulation tests were abnormal in 45% of cats tested (n = 44), few cats showed clinical bleeding tendencies. Most cats received prophylactic vitamin K1 therapy. Forty two cats received aggressive nutritional and supportive care and of these 55% survived. Cats with idiopathic disease were significantly younger, had significantly higher ALP activity and bilirubin concentration, and had a slightly better survival rate than cats with secondary lipidosis. Low PCV, hypokalemia, and an older age were significantly related to nonsurvival. Because of the variety of diets and food supplements used in case management, the influence of nutritional factors on survival could not be evaluated.

Animals↗
PubMedDate not supplied

Ex vivo expansion of bone marrow stromal cells by platelet-rich plasma: a promising strategy in maxillo-facial surgery.

The aim of our study is to evaluate in vitro the response of bone marrow stromal cells (BMSCs) to platelet-rich plasma (PRP), in order to clarify the potential role of their combined use in a preclinical phase preceding BMSCs transplantation for bone repair and regeneration procedures. The incubation of BMSCs with PRP promoted a remarkable, dose- and time- dependent, growth stimulation, that was paralleled to a strong increase in the quantity of type I collagen and to a significant decrease in the activity of the early osteoblastic differentiation marker, alkaline phosphatase (AP). Once PRP was removed and osteogenic inducers were added, AP returned to levels comparable to the control, while the late phenotypic markers, osteocalcin and matrix calcification, were enhanced to higher levels than in controls. Our data demonstrate that PRP induces a remarkable ex vivo enrichment of BMSCs maintaining their differentiative potential. Thus PRP represents a valid preclinical tool for obtaining an effective, rapid and safe ex vivo expansion of BMSCs prior to their clinical utilization in bone engineering.

Bone Marrow Cells↗
PubMedDate not supplied

[Proposed use of natural teeth for management of a prosthetic case].

Authors describe the case of a periodontopathic patient having seriously involved lower incisors. They used a splinting system consisting of a lingual metal bar using close healthy teeth as an anchor, and using previously extracted teeth of the patient to get a good aesthetical, psychological and functional result.

Dental Abutments↗
PubMedDate not supplied

[Laser in periodontology: theoretical-experimental approach. 3. Experimental study of the effects of He-Ne (638 nm) and infrared diodic (904 nm) lasers on the gingival mucosa of rats].

Medical and surgical laser-therapy is becoming more and more important in various branches of Medicine, however independently from clinical results still very little is known about physical-biological interaction between laser beam and living matter. The aim of this study is of finding tissutal and cellular effects of stimulation with soft and mid laser. The study started from an original work carried out at the Institute of Biology of the 1st Faculty of Medicine and Surgery of Naples University from which appears the possibility of visualizing the stage of nuclear active synthesis of the cell, simply by Maloory tricromic colouring. By this method the cellular nucleuses take, usually, a red colour, however in presence of an increase of the RNA nuclear synthesis the nucleuses take a typical blue colour. The importance of the study induced us to research the presence in the oral mucose of the rat after radiation as an effect of biostimulation. The results of this study showed and increase of the epithelial cheratine and a variation of the disposition and of the number of the precheratine grains in treated site. The blue nucleuses have been found at the basal level without any difference between treated and control site. On the other hand their presence is the normal consequence of the epithelial evolution which starts from the basal level which is the most active in the stage of nuclear synthesis.

Animals↗
PubMedDate not supplied

[Water absorption by denture base resins].

In a review of the international literature about water absorption of denture base resins any effect on the physical properties of these polymers has been considered. The authors discuss the possibilities of controlling the final results on denture base function.

Absorption↗
PubMedDate not supplied

[Water absorption by composite resins].

A literature review about water absorption in dental composites has led to a number of hypotheses made for the variables affecting the experimental behaviour. It follows a discussion about the possible advantages and disadvantages of the fenomenon.

Absorption↗
PubMedDate not supplied

[Surgical and nonsurgical therapy: possibilities and limits in treatment of periodontal disease].

Authors expose literature about kinds of periodontal lesions recovery achieved by surgical and non-surgical techniques to establish which of them is preferable and when. They emphasize that, by both techniques, recovery occurs through a "long epithelial attachment" and that is very preferable the surgical technique of tissues guided regeneration gained by membranes that allows the formation of a "new attachment".

Dental Scaling↗
PubMedDate not supplied

[Solubility of luting cements].

An extensive review of the scientific literature for evaluating the current status of luting cements solubility. Both in vitro and in vivo results have been examined and discussed.

Dental Cements↗
PubMedDate not supplied

[Orthodontic treatment and periodontal injury].

The authors examine the literature about the relationship between orthodontic treatment and periodontal tissues health and consider the problems relating to orthodontic therapy in periodontopatic patient.

Animals↗
PubMedDate not supplied

[Diagnosis of the active phase of periodontal disease. 2. Non-clinical parameters].

A review of the Literature shows that a lat of (non-clinical) umoral markers have been suggested as indicators of periodontal disease activity. The advantage of simultaneous evaluation of different markers to obtain a profile of local disease activity has been highlighted in this work as an approach that may provide an usefull picture of the local destructive disease.

Dental Plaque↗