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Biomedical subjects

L Guida

Publications and source records attributed to L Guida.

At least 127 records · Page 7Linked to original sources
PubMedDate not supplied

[Diagnosis of the active phase of periodontal disease. 1. Clinical parameters].

Review of the literature shows that the common clinical parameters used in practice are inaccurate in assessing periodontal disease activity. Radiographs and probings are, instead, relatively accurate in retrospectively assessing periodontal disease activity. Currently more sophisticated radiographic techniques are being developed to diagnose ongoing periodontal disease activity.

Humans↗
PubMedDate not supplied

[Chlorhexidine in the prevention of periodontal disease].

Authors considering microbiological and clinical characteristics of chlorhexidine pointing out its notable capacity to inhibit bacterial plaque formation and analyze possible collateral effects by its use.

Chlorhexidine↗
PubMedDate not supplied

Construction of glucose oxidase-loaded human erythrocytes: a model of oxidative cytotoxicity.

Human red blood cells were loaded with Glucose oxidase from Aspergillus niger by a standardized procedure of encapsulation involving transient hypotonic hemolysis followed by isotonic resealing. The amount of loaded enzyme activity, as evaluated by O2 consumption at 5 mM glucose, ranged from 40 to 75 mumoles O2/hr/ml of packed red cells at 37 degrees C. The red cells loaded with Glucose oxidase were found to behave as efficient glucose-consuming bioreactors. Moreover, at 5 mM glucose, no clear mechanism of H2O2-induced damage was apparent, with the exception of a significantly increased formation of methemoglobin (10% approximately) and of a several-fold stimulated intracellular rate of hexose monophosphate shunt activity, indicating glutathione peroxidase-mediated draining of reduced glutathione for removal of bursts of H2O2. The Glucose oxidase-loaded red cells represent a convenient model system for cytotoxicity studies aiming at clarifying the effects of intracellularly formed H2O2.

Aspergillus niger↗