PubMed Health⌕ Search

Biomedical subjects

L Hammarström

Publications and source records attributed to L Hammarström.

At least 289 records · Page 16Linked to original sources
PubMed1985

Kinetics of the spontaneously occurring common variable hypogammaglobulinemia: an analysis of two individuals with previously normal immunoglobulin levels.

Two males with previously normal serum immunoglobulin levels who spontaneously developed common variable hypogammaglobulinemia were investigated. In one patient serum samples obtained before and after the development of hypogammaglobulinemia were analyzed. A parallel decrease in serum IgM, IgG (all 4 subclasses), IgA (both subclasses) as well as kappa and lambda light chains was found. Serum IgD and IgE also decreased but reached a minimum level before the other immunoglobulin classes. A bone marrow aspirate obtained before the development of hypogammaglobulinemia in this patient showed 1.2% plasma cells, whereas the frequency was only 0.1% when low serum immunoglobulins were recorded. These observations demonstrate the existence of adult onset hypogammaglobulinemia.

Adult↗
PubMed1985

Infectious foci in chronic osteomyelitis of the jaws.

Chronic osteomyelitis of the jaws has a varied clinical appearance and an unclear etiology. In a retrospective study based on case histories and histological material from 24 patients with chronic osteomyelitis, no specific correlation could be found between clinical symptoms and morphological changes in bone. In 10 patients, bacteria were found in certain foci such as osteocytic lacunae of necrotic bone and dentinal tubules of embedded tooth fragments. In these foci, the bacteria probably escaped the immunological response as well as the antibiotic treatment. From these foci, the bacteria might maintain an inflammatory reaction in the surrounding bone. In some patients, the mandibular canal seemed to be a pathway for the spreading of the inflammatory process.

Adolescent↗
PubMed1985

Rabbit anti-human beta 2 microglobulin antibodies: a T-cell mitogen.

Rabbit anti-beta 2 microglobulin antibodies have been described as a potent mitogen for human B lymphocytes. However, when fractionated after activation, only the T-cell enriched population is actively dividing. The induction of proliferation in purified T cells requires the presence of non-T cells. Daudi cells (which do not express beta 2 microglobulin on their cell surface) were shown to produce 'macrophage replacing factors' which supported anti-beta 2-induced T cell division. Non-T cells could be effectively replaced by addition of interleukin 2 containing cell supernatants, 12-O-tetradecanoyl-phorbol-13-acetate (TPA), or teleocidin B. Thus, anti-beta 2 appears to selectively activate T cells. In contrast to other T cell mitogens, anti-beta 2 microglobulin antibodies did not induce secondary immunoglobulin production in B cells from peripheral blood or spleen.

Animals↗
PubMed1985

Ontogeny of IgG2 antibodies against S. aureus teichoic acid in normal and immunodeficient children.

Anti-teichoic acid antibodies of various subclasses were found to be effectively transported across the placenta during pregnancy. In adults these antibodies are mainly of the IgG2 subclass although substantial amounts of specific IgG1 antibodies may also be found. During ontogeny, specific IgG1 antibodies develop during the second year of life whereas specific IgG2 antibodies appear markedly later. In IgG2 deficient children, prolonged deficiency of specific anti-teichoic acid antibodies was observed, suggesting a lack of maturation of the appropriate idiotype(s). In children who received a bone marrow transplant from adult donors, engraftment of IgG2 producing cells could be seen, thus transferring the ability to produce specific antibodies.

Adolescent↗
PubMed1985

Immunoglobulin synthesis in a patient with C4 deficiency: functional immaturity of peripheral blood lymphocytes.

Peripheral blood lymphocytes from a systemic lupus erythematosus patient with homozygous C4 deficiency were stimulated in vitro. Although T cell independent stimulation (Epstein-Barr virus) induced normal proportions of IgM secreting cells. IgG and IgA secreting cells were almost absent. Virtually no immunoglobulin secretion was obtained after T cell dependent stimulation (pokeweed mitogen). Cell fractionation experiments indicated that the low pokeweed mitogen response, at least with regard to IgG and IgA production, was mainly due to impaired B-lymphocyte function. The serum IgG subclass distribution pattern of specific antibodies against certain carbohydrate antigens demonstrated a relative lack of IgG2 antibodies. The findings were consistent with functional immaturity of B lymphocytes in the patient.

B-Lymphocytes↗
PubMed1985

Gm allotypes in IgA deficiency.

Gm phenotypes were examined in 90 Swedish IgA-deficient (less than 0.05 g/litre of serum IgA) donors and 40 normal first and second degree relatives of six of these donors. The G1m1,2, G3m5 and Km1 frequency in the group of IgA-deficient donors did not differ from that found in the normal population. Among the relatives, HLA and/or Gm identical normal sibs were observed. Anti-IgA antibodies were present in 29 of the IgA-deficient donors and anti-IgG in seven. No association between the two was found. A statistically significant association between the G1m-2 phenotype and the presence of anti-IgA antibodies was observed. When subdivided according to HLA type, a non-random distribution of Gm phenotypes was seen in HLA-B8/DR3 positive individuals with anti-IgA antibodies (HLA-B8/DR3 being the haplotype associated with IgA deficiency). These data suggest an association between IgA deficiency, anti-IgA and the studied Gm allotypes.

Dysgammaglobulinemia↗
PubMed1985

Immunoglobulin subclass distribution of human anti-carbohydrate antibodies: aberrant pattern in IgA-deficient donors.

The IgG and IgA subclass distribution of anti-carbohydrate antibodies in normal and immunodeficient donors was investigated. In normal donors, the specific anti-dextran antibodies were mainly of the IgG2 and IgA2 subclass, although substantial amounts of antibodies could also be of the IgG1 subclass. In children, IgG1 was the predominant subclass expressed. An aberrant IgG subclass distribution pattern of specific antibodies occurred in some IgA-deficient donors, with preferential expression of IgG1 and IgG3 anti-dextran antibodies.

Adult↗
PubMed1985

Detection of IgA heavy chain constant region genes in IgA deficient donors: evidence against gene deletions.

Sixty-six donors with selective IgA deficiency and one patient with selective IgA2 deficiency were investigated for immunoglobulin gene defects using restriction enzyme digestions and Southern blot analysis. All patients carried alpha 1 and alpha 2 genes in their genome, suggesting that large deletions are uncommon causes for IgA deficiency. Digestion with Bam HI, Pst I and Pvu II, did not reveal any polymorphism in the studied samples.

Chromosome Deletion↗
PubMed1985

Development of a serologically complete IgA deficiency in a patient with common variable hypogammaglobulinemia.

A male with common variable hypogammaglobulinemia had subnormal but detectable IgA levels (0.2-0.4 g/l). During a period of 1 yr his serum IgA decreased below the detection level to less than 1/10(5) of the original concentration. Serum IgG and IgM were both low but remained constant during the follow-up time. In serum samples lacking IgA, anti-IgA antibodies of the IgG1 subclass were found. His B lymphocytes had a low but detectable capacity to secrete IgA in coculture experiments with purified T cells from healthy donors.

Agammaglobulinemia↗
PubMed1985

Intravenous immunoglobulin in pregnancy.

A woman with variable hypogammaglobulinemia was treated with an intravenous immunoglobulin preparation during pregnancy. The immunoglobulin G1, 2, and 3 subclasses were all transferred to the fetus.

Adult↗
PubMed1984

Purification and characterization of a vanadate-sensitive nucleotide tri- and diphosphatase with acid pH optimum from rat bone.

Rat bone was extracted with KCl and Triton X-100, and a tartrate-resistant acid phosphatase activity was purified by protamine sulfate precipitation, ion-exchange chromatography (CM-cellulose), and gel filtration on Sephadex G-200 according to previously described procedures. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining demonstrated a major band with an apparent monomer molecular size of approximately 14,000 Da. The enzyme is active with p-nitrophenylphosphate (p-NPP) but exhibits a 5- to 10-fold higher affinity towards several nucleotides of which ATP and ADP are the most readily hydrolyzed substrates based on kinetic studies. Based on sensitivity towards proteolytic treatment and detergent removal, as well as pH-optimum studies, a single enzyme was found to be responsible for activity towards nucleotide phosphates as well as p-NPP. This nucleotide tri- and diphosphatase constitutes around 15% of the total acid phosphatase activity in rat bone. The activity with ATP as substrate in contrast to that with p-NPP was inhibited in a noncompetitive fashion by MgCl2, sodium metavanadate, and p-chloromercuribenzoate. Enzyme activity with p-NPP and ATP is dependent on the presence of KCl and detergent and is activated by Fe3+ and ascorbate. The reported characteristics of the enzyme suggest that it functions as a unique membrane acid ATPase.

Acid Phosphatase↗
PubMed1984

Lack of IgG in a healthy adult: a rare case of dysgammaglobulinemia with undetectable serum IgG, IgA2, and IgE.

No IgG of any subclass could be detected in the serum of a normal healthy male adult (E.E.) who had no recent history of repeated infections. Markedly increased concentrations of both IgM and IgA were present although no IgA2 nor IgE could be demonstrated. No anti-immunoglobulin antibodies were present in the serum; when used as supplement in in vitro cultures, the serum supported differentiation of IgG-producing cells to an equal extent as that of normal serum. No cells with surface or intracellular IgG were found in the peripheral blood although low numbers of IgG-secreting cells could be induced in vitro after mitogen stimulation. No increase in suppressor cell activity was found in cocultivation experiments. T-Cell-enriched populations from E.E. were poor inducers of immunoglobulin synthesis in allogeneic B cells, and B-cell-enriched populations from E.E. could not be triggered to synthesize IgG with the aid of allogeneic T cells. Thus, it seems that lack of IgG does not necessarily lead to a highly increased susceptibility to bacterial infections.

Aged↗
PubMed1984

A clinical and radiographic evaluation of cultivated and autotransplanted human teeth.

A vital periodontal membrane (PDM) is of ultimate importance for a successful periodontal healing of auto-transplanted teeth. It has been suggested that a damaged PDM may heal during an intermediate tissue culture period. In the present study, 26 canines, bicuspids and third molars were surgically removed and cultivated in a modified Eagle's medium for 3 to 17 weeks. The teeth were then transplanted to their new positions. 11 out of 18 (61%) transplanted teeth with complete root formation and 7 out of 8 (88%) transplanted teeth with incomplete root formation healed with an apparently normal periodontal ligament. 5 teeth, all canines, became ankylotic. Tissue cultivation of teeth to be transplanted resulted in approximately the same healing rate as has been reported for autotransplanted teeth without the tissue culture procedure.

Adolescent↗
PubMed1984

Tooth ankylosis. Clinical, radiographic and histological assessments.

Ankylosis is a serious condition for the affected teeth as such teeth form part of the remodelling process of the alveolar bone and are therefore progressively resorbed. There are, however, very few clinical studies on tooth ankylosis and the reason for this may be due to the difficulties that are encountered in the diagnosis of minor areas of ankylosis. In the present study, the radiographs, percussion sound and mobility of experimentally extracted and replanted monkey incisors were compared with a morphometric histological study of ankylosis. Ankylotic areas were evident radiographically when the ankylosis was located on the proximal surfaces of the root, but were not evident when the ankylosis occurred on the lingual and labial surfaces. The percussion sound was dull and the mobility normal in all non-ankylotic teeth as well as in those teeth which histologically demonstrated ankylosis on less than 10% of the root surface. When the ankylosis affected 10-20% of the root surface, 2 out of 4 teeth changed their percussion sound from dull to high and these teeth no longer possessed normal mobility. When more than 20% of the root surface was affected with ankylosis, the percussion sound was characteristically high in all teeth and no mobility was present.

Animals↗
PubMed1984

Subclass distribution of human anti-Staphylococcus aureus alpha toxin antibodies: suggestion of an IgG1, IgA1, IgG4 switch pattern.

The subclass distribution of antibodies against alpha toxin from Staphylococcus aureus in man was investigated. Antibodies were restricted to IgG1, IgA1, and IgG4 and appeared to develop sequentially. These data suggest a distinct pattern of class and subclass switches in response to protein antigens. However, studies in immunodeficient (class- or subclass-deficient) donors show that the proposed patterns is not obligatory.

Antibodies, Bacterial↗