Biomedical subjects
Publications and source records attributed to L Ia Severinets.
Opening of the epoxide ring in the molecule of oleandomycin phosphate in the process of its aging was shown to be possible by the data of the analysis. Certain correlation between the "glycol" content and drug activity was detected.
Levorin destruction in oxygen, argon or air at various temperature levels was studied. It was found that destruction of the antibiotic molecule was mainly due to its interaction with oxygen. As a result the heptaenic chromophore was oxidized and triene and tetraene formed. Oxidation of levorin was accompanied by splitting out of paraaminoacetophenone. Destruction of levorin in the absence of oxygen was retarded.
The data on stability of levorin of various purity levels stored under natural conditions and under conditions of thermic rapid inactivation are presented. When the ratio of oxygen to the antibiotic was 1 mole/1 mole the kinetic inactivation curve was of a multistage character with periods of active destruction and quasistatic state. The use of thermic inactivation as a method of accelerated aging is substantiated. A procedure for calculation of the expiry date of the antibiotic is described.
Aureothin, a toxic substance of non-polyenic nature and pentaen 2814-P were isolated from the metabolites of Streptoverticillium mycoheptinicum, the organism producing mycoheptaine. The above substances are also produced by other actinomycetes. The pentaenic antibiotic was divided into 2 components. Physico-chemical and biological properties, as well as the composition of the substances were studied.
Morphological, cultural and physiological properties of actinomycete L10-0208 were studied. It was shown that actinomycete was a new natural variant. It was named Streptoverticillium alboreticuli var. kokandum. The physico-chemical characteristics of the pentaenic antibiotic are presented and its originality is shown. The antibiotic is named kokandomycin.
The kinetics of peroxide formation in the process of levorin and mycoheptin oxidation at a temperature of 57 degrees C was studied. It was shown that the peroxide compounds actively participated in the processes of levorin and mycoheptin inactivation as intermediate products of their transformation. Addition of the peroxides increased the rate of the antibiotic degradation even if there was no oxygen and subsequently decreased the periods of their storage. Conditions for the use of the iodometric method for determination of the peroxides in the poyenic compounds are discussed.
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In the process of growth Streptoverticillium mycoheptinicum, strain 44B/I produced simultaneously a two-component pentaenic antibiotic (mycopenten) and a heptaenic antibiotic (mycoheptin) by the 1st day of cultivation. The ratio of the components remained constant during the whole fermentation process. When grown on the medium with sodium thiosulfate (under conditions of the changed medium potential) the culture produced the heptaen and a mixture of the components with the background absorption of the UV spectrum at 290--360 nm. The ratio of the heptaen and the mixture was also constant during the whole fermentation process.
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Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.
Explore the source record for details and available documents.