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Biomedical subjects

L Komlos

Publications and source records attributed to L Komlos.

At least 37 records · Page 2Linked to original sources
PubMed1988

Primary tissue cultures of benign and malignant human thyroid tumors: effect of TSH.

Many thyroid carcinomas seem to be dependent upon the thyroid growth-promoting properties of the thyroid stimulating hormone (TSH). The purpose of the present investigation was to compare the in vitro effect of TSH on tissue cultures derived from malignant and benign thyroid tumors. The results indicate that TSH can affect the morphology and protein synthesis of primary tissue cultures derived from benign and malignant thyroid tumors differently. The addition of TSH to cultures derived from benign tumors resulted in a reorganization of follicle-like structures of the monolayer and in a reduction of protein synthesis. In contrast to this, monolayers derived from carcinomas of the thyroid were not able to reorganize and their protein synthesis was not inhibited in the presence of TSH. For a better understanding of TSH suppressive therapy, we suggest testing the influence of TSH on a large number of tissue cultures derived from benign and malignant tumors of the thyroid.

Culture Techniques↗
PubMed1987

Differences in sensitivity to melphalan between con A-activated and nonactivated human T-cell subsets.

In vitro treatment with melphalan (L-PAM, L-phenylalanine mustard), 2 micrograms/2 X 10(6) cells, significantly decreased the total number of E-rosette-positive (E+) T lymphocytes from peripheral blood (PBL) of healthy human donors as well as those of the OKT4 (precursor suppressor/helper/inducer T cells) and OKT17 populations (suppressor cells within the OKT4 subset). The OKT8 population (cytotoxic/mature suppressor cells) was not affected by a similar L-PAM treatment. The sensitivity of concanavalin A (Con A)-activated E+ T-cell populations to subsequent L-PAM treatment in vitro was different from that of Con A-untreated T cells: Thus, L-PAM treatment did not affect the expression of OKT3 and OKT4 antigens, increased the percentage of OKT17 cells, and inhibited the expression of OKT8 antigen. Depletion of OKT8 from Con A-activated E+ T cells (OKT4+-OKT8(-)-OKT17+) did not affect their suppressive activity on PHA stimulation in L-PAM-treated as well as untreated cells. Further depletion of OKT17+ cells from the OKT4+-OKT8(-)-OKT17+ subset (OKT4+-OKT8(-)-OKT17-) abolished the suppressive effect on PHA stimulation. Suppressive activity of the OKT4+-OKT8(-)-OKT17- subset was again evident after treatment of this population with L-PAM. The results obtained indicate that the sensitivity to L-PAM treatment of various T-cell phenotypes is changed by Con A activation and that after depletion of specific T suppressor cells L-PAM seems affect the immunoregulatory circuit within the Con A-activated OKT4 subset.

Antigens, Surface↗
PubMed1986

Anterior uveitis, a presenting symptom in acute interstitial nephritis.

Two adult women with idiopathic acute interstitial nephritis (AIN) whose presenting symptom was anterior uveitis are described. On histologic examination of the renal biopsy, eosinophilic infiltrates and mononuclear cells were found. Immunofluorescence was negative. Immunological evaluation showed a slight decrease in the number of T cells with a normal subpopulation ratio. AIN responded to steroid treatment.

Acute Disease↗
PubMed1986

The immunomodulating effect of theophylline on lymphocytes from chronic lymphocytic leukemia patients.

The in vitro immunomodulating effects of theophylline on E-rosette formation, phytohemagglutinin (PHA) response, and Ig surface receptors of B lymphocytes were studied on fresh as well as on preincubated lymphocytes from patients with B cell chronic lymphocytic leukemia (CLL). In 11 out of 14 CLL patients, 24 hours preincubation at 37 degrees C significantly enhanced E-rosette formation. Subsequent treatment of preincubated cells with appropriate concentrations of theophylline further enhanced E-rosette formation in 11 cases. On fresh lymphocytes the enhancing effect of theophylline on E-rosette formation was not significant. The same was true for PHA stimulation; in 5 out of 7 cases the mitogen enhanced the stimulating effect of preincubation and had no significant effect on fresh lymphocytes from CLL patients. Preincubation significantly reduced the percentage of surface immunoglobulin positive B cells from CLL patients in all cases studied, and theophylline treatment had an additional effect on this phenomenon. No such effect of theophylline on fresh B cells from CLL patients could be observed. Preincubation had no significant effect on control lymphocytes. The effect of theophylline on control lymphocytes as compared to lymphocytes from CLL patients was completely different for T as well as for B lymphocytes. E-rosette formation from control lymphocytes (fresh and preincubated) was significantly inhibited in the presence of theophylline. No significantly enhanced responsiveness to PHA could be observed after treatment of fresh or preincubated lymphocytes with theophylline. Preincubation and theophylline treatment had no significant effect on the percentage of Ig positive B cells from control lymphocytes.(ABSTRACT TRUNCATED AT 250 WORDS)

Adjuvants, Immunologic↗
PubMed1985

In vitro selective effect of melphalan on human T-cell populations.

In vitro treatment with 2 micrograms/2 X 10(6) cells melphalan (L-PAM: L-phenylalanine mustard) significantly decreased the total number of T lymphocytes from peripheral blood (PBL) of healthy human donors and of the OKT4 population (precursor suppressor/helper/inducer) T cells as defined by monoclonal antibodies OKT3 and OKT4, respectively. No changes in the OKT+8 lymphocyte population (cytotoxic/mature suppressor cells) were observed following the same treatment. Preincubation of PBL with L-PAM at concentrations that do not affect the rate of DNA synthesis in PHA-stimulated lymphocytes inhibited the generation of T suppressor lymphocytes by ConA, as shown by their effect on PHA stimulation. Treatment of allogeneic PBL with L-PAM had no effect on mature suppressor T cells already induced by ConA, as shown by incubation of PBL with L-PAM after incubation with ConA.

Antibodies, Monoclonal↗
PubMed1985

Sialic acid in newborn and maternal lymphocytes: preterm deliveries.

Sialic acid was determined in newborn and maternal lymphocytes immediately after preterm deliveries of 34 and 35 weeks of gestation. The results were compared with those found in full-term deliveries. In contrast with full-term deliveries, in preterm deliveries a significant increase in sialic acid in maternal as compared to newborn lymphocytes was found. However, the mean cumulative value of sialic acid for maternal and newborn lymphocytes was similar in both groups. In addition to this, sialic acid concentrations seem to be sex-dependent and higher mean cumulative values for maternal and newborn lymphocytes could be found for male as compared to female newborns after full- as well as after preterm deliveries.

Female↗
PubMed1984

Synergistic effect of N-acetyl-D-glucosamine (NAG) on mitogenic, antigenic and allogeneic stimulation of normal human lymphocytes.

The effect of N-acetyl-D-glucosamine (NAG) on in vitro stimulated human peripheral blood lymphocytes was investigated. NAG was added to lymphocyte cultures stimulated by the mitogen PHA, the antigen PPD or to one-way-stimulated mixed lymphocyte cultures from unrelated donors. The results indicate that NAG (1-2 micrograms/ml) in appropriate experimental conditions has an enhancing effect on DNA and protein synthesis induced by PHA, PPD and allogeneic cells. Addition of NAG (1-2 micrograms/ml) to unstimulated lymphocyte cultures had no effect on DNA or protein synthesis.

Acetylglucosamine↗
PubMed1983

Sialic acid in lymphocytes and sera from mothers and their newborns after normal deliveries.

Sialic acid was determined in newborn and maternal lymphocytes as well as in newborn and maternal serum immediately after normal deliveries. A significant increase in sialic acid in newborn as compared to maternal lymphocytes was found. Sialic acid concentration seems to be sex-dependent for newborn lymphocytes only, and higher concentrations of sialic acid were observed in male as compared to female newborn lymphocytes. No differences between the sialic acid concentration in maternal and control lymphocytes could be found. In newborn serum, sialic acid was significantly reduced as compared to maternal or control serum. No sex-linked differences were observed. No differences between the concentration of maternal and control serum were detected.

Female↗
PubMed1983

Auto-rosette formation in the peripheral blood of a patient with infectious mononucleosis.

Auto-rosette formation by peripheral blood lymphocytes was observed in a patient with infectious mononucleosis (IM), with the scanning electron microscope (SEM). The red blood cells adhered to lymphocytes with numerous microvilli on their surface. This phenomenon was observed only during the acute stage of the disease and not in the recovery period. Auto-rosette formation was detected neither in 7 other patients with IM, nor in control subjects.

Child↗
PubMed1980

Ultrastructural studies on the lymphocyte subpopulations of disaggregated mouse spleens.

The lymphocyte subpopulations of disaggregated spleens of adult C57Bl/6J mice were examined by transmission and scanning electron microscopy as well as by rosette formation. The results showed that most lymphocyte subpopulations are constituted of cells with relatively smooth surfaces. Rosette formation showed that the proportion of E rosettes, compatible with the presence of T cells, was of 25%, whereas that of erythrocyte antibody complement (EAC) rosettes, indicating B cells, was 9%.

Animals↗
PubMed1979

Repeated abortions and histocompatibility antigens. Can HLA antigen restricted gene dose effects influence the feto-maternal relationship?

It is suggested that the induction of cytotoxic reactions in the mother by allogeneic fetal cells is HLA antigen restricted and influenced, in certain haplotype combinations, by gene dose effects of specific HLA antigens (locus A and B), shared by the fetus and by the mother. The possibility that alloimmunization of maternal cells to fetal transplantation antigens may have beneficial or adverse effects on the fetal target cells dependent upon feto-maternal histocompatibility antigen interrelationship would provide a new approach for interpreting functional disturbances in certain cases of human pregnancy.

Abortion, Spontaneous↗
PubMed1978

The effect of sera from chronic lymphocytic leukemia patients on normal mitogen-stimulated lymphocytes. A possible correlation with the patients' T and B lymphocytes.

Serum from chronic lymphocytic leukemia (CLL) patients was examined for its effect on normal lymphocytes stimulated by phytohemagglutinin and pokeweed. Responses of the patients' lymphocytes were similarly examined. E and EAC rosettes were also tested. A significantly increased transformation response was found when normal lymphocytes were exposed to sera from those patients who had a reduced number of E-rosette-forming lymphocytes (mean 8%) as compared to the response to sera from patients with a higher number of E-rosette-forming cells. The results suggest that a factor is present in sera from some CLL patients which stimulates the transformation of mitogen-stimulated lymphocytes and which seems to be related to the number of the patient's T and B lymphocytes and perhaps with a T suppressor effect on B lymphocytes.

Aged↗
PubMed1977

Lymphocyte reactivity in the aged.

Lymphocyte reactivity in cultures toward the nonspecific T and B cell mitogens, phytohemagglutinin (PHA) and pokeweed (PWM), was studied in 46 subjects in three groups: a) young healthy persons, b) elderly healthy persons, and c) hospitalized elderly patients with atherosclerotic cardiovascular disease. No statistically significant differences in the lymphocyte reactivity of young and old healthy persons toward PHA could be observed with different incubation times, mitogen concentrations, or serum sources. A significantly decreased lymphocyte response was found in elderly hospitalized patients compared to young and elderly healthy subjects. In contrast, no differences in the lymphocyte reactivity toward PWM could be found among the three groups, including a comparison between elderly healthy and elderly hospitalized persons. The results suggest that the B lymphocyte-dependent response to a B lymphocyte stimulator is more stable than is the T cell response in aged subjects.

Adolescent↗