Second form in a segment of the merozoite surface protein 1 gene of Plasmodium vivax among isolates from Rondônia (Brazil).
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Amino Acid Sequence↗
Biomedical subjects
L M Camargo
Publications and source records attributed to L M Camargo.
[Mortality due to Chagas disease in São Paulo State (Brazil): subsidy for the planning of chagasic patient care].
In order to estimate the size of the population with Chagas' disease, an essential parameter for the establishment of an adequate health care policy for this group of patients, data from 1,646 death certificates from the "Fundação Sistema Estadual de Análises de Dados" in which Chagas' disease was mentioned in the State of S. Paulo in 1987 were examined. The population under study comprised adults between 15 and 70 years of age. Objectives also included the determination of Proportional Mortality, Potentially Lost Years of Life and regional distribution of the events in the counties of the State of S. Paulo. Chagas disease accounted for 0.9% of the totality of the causes of death, Proportional Mortality was 1.2% and the proportional participation of Chagas' disease in the Potentially Lost Years of Life was 1.1%. Corresponding data for Ischemic Heart Disease were, 4.0% and 1.2%. 20.8% of the deaths occurred in counties other than the county of residence, the indexes of evasion varying from 17.8% to 29.1% for different regions. Males (22.8%) more often than females (17.4%) died outside their county of residence. The largest number of deaths occurred in the metropolitan area of S. Paulo, although Ribeirão Preto county presented the highest mortality indexes.
Adolescent↗
[Anemia in a population from an endemic area of malaria, Rondônia (Brazil)].
With the purpose of describing the prevalence rate of anaemia among inhabitants of a malaria endemic area--Candeias district, a periurban locality near Porto Velho, in Rondônia State, Brazilian Amazon Basin--a random population sample comprehending 1,068 individuals of all age groups (14.1% of the total population) was screened for anaemia (measurement of blood haemoglobin concentration) and malaria (Giemsa-stained thick-smear microscopy). Two-hundred and ninety-nine individuals (28.0% of the sample) were found to be anaemic, using the cut-off haemoglobin values proposed by the World Health Organization for each age group. Highest prevalence rates were found among children with ages varying from 6 months to 1 year (70.0%) and from 1 to 6 years (38.4%), as well as in pregnant women (41.2%, 7/17) and malaria patients (44.4%, 8/18). Parasitological stoll examinations were made on a voluntary sample of 476 individuals (44.6% of the sample population); of these, 118 (26.8%) were positive. Eggs of Ascaris lumbricoides, the most frequent intestinal parasite in this population sample, was detected in 67 stool samples (14.1%); only 27 patients (5.7%) eliminated Ancylostomidae eggs. In this voluntary sample, no significant difference in anaemia prevalence rates between parasite carriers and non-parasited individuals was detected. On the other hand, the more recent the last malarial episode referred to by the patients, the lighter prevalence rate of anaemia in individuals above the age of 14 years. The role played by malaria as an underlying cause of anaemia in Candeias district inhabitants, particularly in the economically active age group, is further discussed.
Adolescent↗
Human IgG responses against the N-terminal region of the Merozoite Surface Protein 1 of Plasmodium vivax.
The complete primary structure of the gene encoding the Merozoite Surface Protein 1 of Plasmodium vivax (PvMSP-1) revealed the existence of interspecies conserved regions among the analogous proteins of other Plasmodia species. Here, three DNA recombinant clones expressing 50, 200 and 500 amino acids from the N-terminal region of the PvMSP-1 protein were used on ELISA and protein immunoblotting assays to look at the IgG antibody responses of malaria patients from the Brazilian amazon region of Rondônia. The results showed the existence of P. vivax and P. falciparum IgG antibodies directed against PvMSP-1 antigenic determinants expressed in the clones containing the first 200 and the following 500 amino acids of the molecule, but not within the one expressing the most N-terminal 50 amino acids. Interestingly, there was no correlation between the levels of these IgG antibodies and the previous number of malaria infections.
Animals↗
Intestinal parasites among Karitiana Indians from Rondônia State, Brazil.
Gravity sedimentation parasitological examinations were performed in stool samples from 111 Karitiana Indians from Rondônia State, Brazilian Amazon Basin. Intestinal parasites were found in 43 samples (38.7%). Ascaris lumbricoides was the most prevalent helminth species (18.9%). Egg counts in samples positive for Ascaris suggested an overdispersed distribution of worm burdens in the host population. Age-distribution pattern of intestinal parasites among Karitiana Indians was found to be rather unusual: the highest prevalence (60.0%) was detected in the 12- to 16-year-old age group.
Adolescent↗
Intestinal parasites in Iaualapiti Indians from Xingu Park, Mato Grosso, Brazil.
Brine flotation and gravity sedimentation coproscopical examinations were performed in stool samples from 69 of the 147 Iaualapiti Indians of the Xingu Park, Mato Grosso State, Brazil. Intestinal parasites were present in 89.9% of the population examined. High rates of prevalence were found for some parasite species: Ancylostomidae, 82.6%; Enterobius vermicularis, 26.1%; Ascaris lumbricoides, 20.3%; and Entamoeba coli, 68.1%. Infection by Trichuris trichiura, Schistosoma mansoni, Taenia spp. and Hymenolepis nana was not detected. Helminth's prevalence in children aged one year or less was comparatively low (33.3%). Quantitative coproscopy was done in positive samples for Ascaris and Ancylostomidae and the results expressed in eggs per gram of feces (EPG). Quantitative results revealed that worm burdens are very low and overdispersed in this Indian tribe, a previously unreported fact.
Adolescent↗
Using a microplate reader to quantify dot immunobinding assays.
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Immunoblotting↗
Malaria seroepidemiology: comparison between indirect fluorescent antibody test and enzyme immunoassay using bloodspot eluates.
Blood sampling on filter paper is a current practice in malaria seroepidemiological studies by indirect fluorescent antibody test (IFAT). There is, however, scant comparative information about the use of bloodspot eluates for detection of malarial IgG antibodies simultaneously by IFAT and enzyme immunoassay (ELISA). Here we report data obtained by both serological methods done on 219 bloodspot eluate samples collected in a rural community in Brazilian Amazon Basin (Alto Paraíso, Ariquemes municipality) where malaria is endemic. Plasmodium falciparum and P. vivax thick smear antigens were used in the IFAT; a detergent-soluble P. falciparum antigen was prepared for ELISA. Substantial agreement of results (Kappa coefficient k = 0.686) was observed when P. falciparum antigen was used in both tests, and IFAT titers were found to be strongly correlated to ELISA antibody units (Spearman correlation coefficient rs = 0.818, p < 0.001). Only moderate agreement (k = 0.467) between IFAT with P. vivax antigen and ELISA with P. falciparum antigen was observed. Spearman correlation coefficient value between quantitative results (IFAT titers and ELISA antibody units) in this case was numerically lower (rs = 0.540, p < 0.0001). Our results suggest that, with P. falciparum antigen, both IFAT and ELISA performed on bloodspot eluates are equivalent for seroepidemiological purposes.
Antibodies, Protozoan↗