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PubMed · 1842439

Using a microplate reader to quantify dot immunobinding assays.

M U Ferreira·A M Stolf·C S Ferreira·A M Katzin·A M Ramos·E Kimura·S M Di Santi·L M Camargo
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M U Ferreira, A M Stolf, C S Ferreira, A M Katzin, A M Ramos, E Kimura, S M Di Santi, L M Camargo. Using a microplate reader to quantify dot immunobinding assays.. https://doi.org/10.1590/s0074-02761991000400017

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Ssn6-Tup1 is a general repressor of transcription in yeast.

The homeodomain protein alpha 2 and the SRF-like protein Mcm1 are required to establish cell type in the yeast Saccharomyces cerevisiae. Together, these regulatory proteins recognize a specific DNA operator, marking a set of genes for transcriptional repression. In this paper, we show that occupancy of the operator by alpha 2-Mcm1 is not sufficient to bring about repression. Rather, repression is effected only when Ssn6 (a TPR protein) and Tup1 (a beta-transducin repeat protein) are also present in the cell. We show that Ssn6 represses transcription when brought to a promoter by a bacterial DNA-binding domain and that Tup1 is required for this repression. Based on these and other results, we propose that Ssn6-Tup1 is a general repressor of transcription in yeast, recruited to target promoters by a variety of sequence-specific DNA-binding proteins.

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