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Biomedical subjects

L Mosci

Publications and source records attributed to L Mosci.

9 recordsLinked to original sources
PubMed1979

[Technic for studying antitetanus immunity].

The Authors carried out a research work on problems relating to technical aspects to the tetanus immunity tests. Some interesting conclusions have been reached: - Purified and diluted tetanus toxin is best preserved at - 20 degrees C, in pH 7.4 physiological salt solution added with 1% peptone. - Vaccination in small laboratory animals must be done injecting adsorbed anatoxin by a microsyringe, in order to avoid dilution of the original compound. - Protection test using low, i.v. toxin doses is more reliable than serological tests in assessing the acquired resistance level in immuno-prophylaxed animals. - Passive haemagglutination test is much more sensitive when done with turkey instead of sheep red blood cells.

Animals↗
PubMed1977

Enhanced early response to tetanus toxoid, in rabbits.

AIPO4 adsorbed tetanus toxoid injected in rabbits according different vaccination schedules, gives rise to antibody responses with varying lag periods. Shortening the intervals among antigen injections, or increasing the quantity of toxoid per dose, makes it possible to reach protective serum antibody levels in 10 days. That is to say, in a period of time shorter than the mean tetanus incubation in man. Although difficult to transfer to man, the exposed data suggest a possible differentiated antitetanus vaccination in subjects at risk, non protected.

Animals↗
PubMed1977

Anti-Candida albicans precipitating antibodies in the sera of surgery patients.

In healthy subjects and in subjects undergoing surgical intervention for neoplastic and other diseases, the behaviour of precipitating antibodies against soluble Candida albicans natigens was studied. In 13,7% of healthy controls the Ouchterlony test was positive. In subjects suffering from intestinal tract neoplasia, the percentage of positive tests was 26,6% before and 66,6% after the intervention. In other two groups of patients affected by neoplasia, the per cent positive results of the tests after intervention were, respectively, 50% and 63,1%. Among non neoplastic patients undergoing surgical treatment, after intervention 35% too proved positive. The data were confirmed by P.E.T. (partigen elution test): specific anti-Candida IgG, and in some cases IgA, could be demonstrated. The diagnostic value of positive Ouchterlony's test against superficial soluble Candida antigens after surgical intervention has been discussed.

Antigens, Fungal↗
PubMedDate not supplied

[Some experimental data on the seroprophylaxis of tetanus].

The survival time of tetanus heterologous antitoxins, crude or digested, has been controlled by indirect haemagglutination (I.H.A.) and neutralization tests in guinea pigs and rabbits. Digested antitoxins are demonstrable in guinea pigs up to the 8th day, in rabbits only during the first day. They reach the highest level 24 hours after the injection: tested with I.H.A. show a good correlation with the quantity of injected antitoxin. In the sera of three wounded subjects, treated with Ig or digested horse antitetanic serum, after 24 hours the I.H.A. test was negative, while the neutralizing antibody titer was equal to 0.1/0.01 I.U./ml. These contrasting results are probably due to circulating Fab' gragments from decaying tetanus antitoxins.

Animals↗
PubMedDate not supplied

[Tetanus prevention with vaccine and with vaccine plus heterologous immune serum: serum antibody levels in the rabbit].

Haemagglutinating antibodies have been assessed in rabbits undergoing active- passive immunization against tetanus. The animals received 6 injections of horse immune serum, 400 UI/kg, and A1PO4 adsorbed toxoid, 0.35 Lf/kg, every 30th day. One the 5th day, after the first injection, E.A. antibodies appeared, at low levels, as a result of a passive immunization. Thereafter the tests became negative, up to the 70th day, when an active immunization emerged, with a 25 days delay in comparison with controls. Neutralization test in vivo behaved in the same way. The results stress once more the need to give up the use of heterologous immune sera in tetanus prophylaxis, in active-passive immunization as well. Arguments adding force to this point of view are: the sensibilization against heterologous proteins, the very low (if any) passive protective action, and, last not least, the delay in the emergence of active immunization: the only reliable shield against tetanus.

Animals↗