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L N Button

Publications and source records attributed to L N Button.

26 records · Page 2Linked to original sources
PubMedDate not supplied

Rhesus monkey platelet kinetics and storage.

Rhesus monkey platelets that are separated, 51Cr labeled, and transfused provide yields, 1/2 time and survivals similar to those obtained in man. Rhesus platelets prepared and stored at room temperature for three days have 93 per cent of the yield of fresh platelets. When prepared and stored at 4C, the yield is only 54 per cent of fresh platelets. Rhesus repeatedly transfused with platelets from random unmatched donors always have a significant decrease in the platelet 1/2 time and survival. This was observed after the second homologous transfusion in one and after the third in others. The rhesus provides an excellent platelet model with thrombocyte responses mimicking those of man.

Animals↗
PubMedDate not supplied

The in vivo survival of red blood cells stored in modified CPD with adenine: report of a multi-institutional cooperative effort.

In order to provide data in support of licensure applications for citrate-phosphate-dextrose (CPD) supplemented with adenine, a multi-institutional cooperative effort was organized to determine survivability of red blood cells subjected to prolonged liquid storage. Two manufacturers supplied plastic multiple bag blood storage containers prefilled with modified CPD (glucose 25% greater than the normal concentration) supplemented with adenine (17.0 to 17.3 mg per 63 ml of anticoagulant; 0.25 millimolar approximate final concentration when diluted with 450 ml of whole blood for 35 days showed a mean survival of 80.53 +/- 6.44 per cent (1 SD). Both red blood cell and supernatant plasma biochemical characteristics were comparable to those reported for whole blood stored for 21 days in either acid-citrate-dextrose (ACD) or CPD. Red blood cells from 19 units stored as concentrates for 35 days (Hct 75.03 +/- 3.74%) had a mean survival of 71.38 +/- 10.3 per cent with considerable interdonor variation in survival and interlaboratory variation in some biochemical characteristics. Red blood cells from eight units stored as concentrates (Hct 75.38 +/- 4.30%) for 28 days showed a mean survival of 83.97 +/- 6.10 per cent and biochemical characteristics comparable to those reported for red blood cell concentrates stored in CPD or ACD for 21 days. Modified CPD with adenine as formulated offers an improved anticoagulant for blood banking by extending the permissible red blood cell storage period.

Adenine↗
PubMedDate not supplied

The effects of irradiation on blood components.

The functional properties of formed elements of whole blood were studied following irradiation doses of 500 to 20,000 rads. Irradiated lymphocytes retained only 1.5 per cent of their 3H thymidine uptake after a 5,000-rad exposure and none after 7,500 rads. Red blood cells stored for 21 days and then irradiated with 5,000 rads had the same survival as nonirradiated controls. In contrast, 5,000 rads reduced platelet yields. However, transfused irradiated platelets produced the expected increases in platelet counts and controlled hemostasis in thrombocytopenic patients. After 5,000 rads, granulocytes had normal bacterial killing capacity, chemotactic mobility, and normal superoxide production after high-dose stimulation. Nitroblue tetrazolium reduction and ingestion stimulated by complement opsonized oil droplets were not diminished by 5,000- and 10,000-rad irradiation. The functional qualities of cellular blood components other than lymphocytes are not compromised by 5,000 rads. This irradiation dose may be an effective means of controlling incidence of graft-vs-host disease in immunosuppressed patients.

Blood Cells↗
PubMedDate not supplied

Proposed standardization of methods for determining the 24-hour survival of stored red cells.

Two methods of determining the survival of stored red cells are described: one using a double label and one using a single label. It is not suggested that one is superior, but rather both methods are presented in the hope that the personnel of laboratories engaged in determining the effectiveness of anticoagulant-preservation will follow one of the protocols presented. Were this to occur, many of the interlaboratory variations that have decreased the value of data from red cell survival studies would be decreased.

Anticoagulants↗