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Biomedical subjects

L N Chernousova

Publications and source records attributed to L N Chernousova.

At least 55 records · Page 3Linked to original sources
PubMed1994

[Humoral immune response in humans and animals infected and immunized with Mycobacteria of the MAIS complex].

Hyperimmunization of mice and rabbits with surface (whole cells) and complex (ultrasound disintegrants) with the MAIS complex Mycobacterium antigens induced a humoral immune response which was largely aimed against cross-reacting than "unique" Mycobacterium antigens. Infection of BALB/c mice and rabbits with M. avium induced a humoral immune response which was chiefly against cross-antigenic Mycobacterium determinants. A series of consecutive immunochemical procedures were used to isolate from M. avium antiserum the antibody reacting with the antigenic determinants 18, 20, 24, 27, 39, 68, 94, kDa. Mab to the antigenic determinant 79 kDa of M. avium, which belong to the IgG class and unreacting with antigens of other Mycobacterium types were obtained. The antibodies isolated from hyperimmune serum and Mabs were used in the competitive EIA to determine antimycobacterial antibodies in man, mice, and rabbits. With Mab, positive results were obtained in 75% of M. avium-infected mice, 63.6% of rabbits and 63.6% of patients with M. avium infection (with 5.0% false positive results in humans). With antiserum, 83.3% of positive results were obtained in mice (with 10.0% of false positive results), 81.8 in rabbits (with 25% of false positive results) and 90.9% in M. avium-infected persons (with 30% of false positive results).

Animals↗
PubMed1994

[Identification of mycobacteria in sputum by determining antigens in the culture medium].

Antigens to M. tuberculosis in culture fluid of sputum samples were compared to bacterioscopy and inoculation results for 64 new-onset cases of tuberculosis and 60 chronics with bronchitis. The authors used diagnostic preparations of monoclonal and polyclonal antibodies to M. tuberculosis. The use of the above preparations in the enzyme immunoassay reduced the time of M. tuberculosis detection in the patients' sputum and increased the frequency of the bacteria registration against standard methods of bacterioscopy and inoculation.

Antibodies, Monoclonal↗
PubMed1993

[Level and spectrum of tuberculosis antibodies in mice sensitive and resistant to tuberculosis].

The level and spectrum of antituberculous antibodies (AA) were assessed in mice and their hybrids sensitive or resistant to tuberculosis. The AA level in resistant animals (CBA) and hybrids (CBA x C57Bl/6) F1 surpassed that in the sensitive mice (C57Bl/6). The spectrum in resistant and hybrid mice appeared more varied than that in the sensitive animals. Resistant mice had bands in the range 15-20 kD.

Animals↗
PubMed1993

[Production of purified antigenic drug from Mycobacterium tuberculosis humanus and its use for serodiagnosis of tuberculosis].

A variety of antigenic preparations from whole bacteria M. tuberculosis humanus (H37Rv) and relevant subcellular components have been tested using indirect ELISA for determination of antituberculous antibodies. Cell membranes of mycobacteria and antigens isolated from KCl-extracts of cell membranes in gel Toyopearl HW 55 F demonstrated the highest activity. Fraction 6 usage raised the reaction sensitivity to 83.3% in specificity 86.7%. As indicated by the ELISA, the highest antibodies content under the above antigen introduction was reached in patients with fibrocavernous tuberculosis, the lowest one in focal tuberculosis.

Antibodies, Bacterial↗
PubMed1993

[The isolation of monoclonal antibodies to strains of Mycobacterium leprae passed through laboratory animals].

Hybridoma synthetizing monoclonal antibodies (mAB) IIIE4 has been obtained in hybridization of myeloma P3-X63-Ag8. 653 cells and splenocytes from BALB/c mice immunized with M. leprae passaged on rats. The mAB specificity evaluated by enzyme immunoassay using ultrasonic disintegrates of M. leprae obtained from human, Dasypus novemcinctus, rat lepromas as well as mycobacteria of 7 species and E. coli indicated that the mAB reacted only with mycobacteria passaged on the rats. They had weak cross reactivity with M. avium.

Animals↗
PubMed1993

[The level and spectrum of antituberculosis antibodies in BCG-vaccinated mice and in mice and rabbits infected with a virulent mycobacterial culture].

The authors examined CBA mice infected with a virulent culture (H37 Rv), BCG-vaccinated mice and intact mice and rabbits infected with a virulent culture Bovinus-8 after preliminary sensitization. It was established that the level of antituberculous antibodies in the enzyme-linked immunoassay was higher in H37 Rv infected mice than in BCG-vaccinated animals (regardless of the used antigen, the ultrasonic disintegrator BCG or H37 Rv). The spectrum of antituberculous antibodies was different in the infected and vaccinated mice, the infected mice showed additional bands in the 20 kD area. The level of antituberculous antibodies was higher in rabbits with a limited process than in those with a disseminated process. With the latter, antibodies to antigenic determinants that had lower molecular weight (25-35 kD) were detectable.

Animals↗
PubMed1991

[The characterization of the spectrum of the antibody response to Mycobacterium tuberculosis antigenic determinants by immunoblotting].

The spectrum of antibody response to M. tuberculosis antigenic determinants H37Rv and M. bovis antigenic determinants BCG was studied in serum samples from 33 healthy donors and 31 patients with infiltrative pulmonary tuberculosis by the method of immunoblotting. The study revealed that most frequently tuberculosis patients showed response to Ag-H37Rv with molecular weights of 52, 39, 35, 21, 31, 68 kD (44.4-22.2%) and Ag-BCG with molecular weights 60, 58, 50, 25, 54, 70 kD. (33.3-22.2%). By month 9 of effective chemotherapy binding predominantly with Ag-H37Rv determinants of 31, 62, 35, 75, 56, 28, 19, 5, 13 kD (75-37.5%) and Ag-BCG determinants of 13, 34, 38, 44, 19, 36, 45, 52, 58, 60 70 kD (37.5-25%) were registered. Some differences in the spectra of antibody response to Ag-H37Rv and Ag-BCG determinants were noted.

Antibodies, Bacterial↗
PubMed1990

[Production and characteristics of monoclonal antibodies reacting with human-type M. tuberculosis].

Monoclonal antibodies to M. tuberculosis were produced by hybrid technology. They were described via enzyme immunoassay and immunoblotting. It was shown that these antibodies should be included into IgG class. Besides, they are oriented towards and antigen with a molecular weight of 20 kDa, react with H37Rv at a concentration of 12 ng/ml and with BCG at a concentration of 50 micrograms/ml and fail to react with M. Intracellulare, M. Scrofulaceum and E. coli.

Animals↗
PubMed1980

[Effect of antiserum against isologous aggregated immunoglobulins on synthesis of nonspecific immunoglobulins in mice immunized with sheep erythrocytes].

Injection of antiserum against isologous aggregated immunoglobulins into mice immunized with sheep red blood cells (5X10(7)) suppressed by 20% on the average the synthesis of nonspecific immunoglobulins. Concurrently the antiserum caused a 70% decrease in the amount of rosette-forming cells, but did not affect the proliferation of anti-body-forming cells and hemagglutinin production. The data obtained warrant an assumption that the part of B lymphocytes bearing aggregated antibodies are responsible for the synthesis of nonspecific immunoglobulins.

Animals↗
PubMed1979

[Effect of antiserum against isologous aggregated immunoglobulins on the delayed-type hypersensitivity in mice immunized with sheep red blood cells].

The mouse antiserum against isologous aggregated immunoglobulins (MAAS) injected to mice sensitized with 10(5) sheep red blood cells (SRBC) did not influence the delayed-type hypersensitivity (DTH) tested on the peak of sensitization (the 4th day) but enhanced significantly DTH tested on the 6th day. MAAS completely abolished the DTH suppression observed after sensitization with 5 x 10(7) SRBC. In transfer experiments the number of the DTH suppressor cells decreased in the spleen of sensitized mice under the MAAS action. MAAS did not affect the proliferation of antibody-forming cells (AFC) and hemagglutinin production but reduced by 70% the number of rosette-forming cells (RFC) in the spleen on the peak of the initial immune response. The data obtained may indicate that RFC participate in DTH suppression.

Animals↗
PubMed1978

[Effect of mouse antiserum against isologous aggregated immunoglobulins on the accumulation of rosette- and antibody-forming cells in mice immunized with ram erythrocytes].

The paper describes the effect of mouse antiserum against isologous aggregated immunoglobulins (termed MAAS) on the kinetics of rosette-forming and antibody-forming cells (RFC and AFC, respectively) in mice immunized with SRBC. MAAS effect was assessed in vivo by injecting this serum for 5 days to mice CBA, combining the first injection with the injection of 5.10(7) SRBC. MAAS administration to mice immunized with SRBC induced a marked reduction of RFC in the spleen on the 5th and 9th days after the immunization. At the same periods MAAS produced no significant effect on the proliferation of AFC producing IgM-hemagglutinins. At the same time MAAS intensified the IgG-AFC proliferation in the period of the maximal content of these cells in the spleen of the immunized mice. After the MAAS absorption with the immune complexes formed by the mouse IgG-antibodies this serum largely lost its capacity to block RFC in vivo. On the basis of the data obtained it is suggested that the property of MAAS to influence the accumulation of RFC and AFC producing IgG-hemagglutinins is caused by the factor reacting with the immune complex formed by mouse IgG-antibodies. Possibly this factor represented antibodies against the aggregated immunoglobulins of this class.

Animals↗