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Biomedical subjects

L N Filippenko

Publications and source records attributed to L N Filippenko.

At least 19 recordsLinked to original sources
PubMed1988

[Morphological heterogeneity and functional status of the alveolar macrophages from bronchoalveolar lavage during the development of tuberculous inflammation in guinea pigs].

Alveolar macrophages (AM) isolated from bronchoalveolar lavage of guinea pigs obtained in the course of generalized tuberculosis development was studied by electron microscopy. The protein content, activity of cathepsins B and D, as well as macrophage and neutrophil elastase activity have been determined. It was shown that during the first month of specific process development an influx of young biosynthesizing AM building up the lysosomal apparatus was observed; phagocytic, digesting and secretory functions of mature cells were enhanced. Progressing of tuberculosis was accompanied by the inhibition of biosynthetic processes in AM, a decrease in digesting and secretory functions, which were manifested at the biochemical and then at the electron microscopic levels and were of a prognostic value.

Animals↗
PubMed1984

[Ultrastructure of neurons of the myenteric plexus in the normal cat and after temporary circulatory interruption].

Ultrastructural organization of neurons in the myenteric plexus of the cat small intestine has been studied in norm and after the blood stream had been switched off for 4 hours and 1, 3, 7, 30 days after recirculation. Ultrastructural uniformity of perikaryons is noted, therefore it is impossible to reveal electron microscopically neurons with different functional qualities. Four morphological types of preterminal dilatations of axons are revealed. Some neurons demonstrate certain resistance to the four hours' ischemia of the intestine, predominance of reactive processes in most of the cells. Dynamics of destructive and reparative processes are followed in neurons and their processes after recirculation.

Animals↗
PubMed1981

[Electron microscopic demonstration of pulmonary surfactant proteins using procion brilliant blue H5GS].

A new electron microscopy technique is described for detection of lung surfactant proteins with the copper-containing phthalocyanine dye, procion brilliant blue H5GS. The protein structures were stained concurrently with the fixation during perfusion through the pulmonary artery of a fixative-staining mixture containing glutaric aldehyde and a dye in the kakodilate buffer, pH 5.6-6, and in the course of a subsequent immersion of lung tissue pieces into the same mixture. Then the material was treated with thiosemicarbazide and post-fixed with OsO4. The dye did not penetrate intact cells. The electron-dense products of the histochemical reaction were located inside and on the surface of the surfactant membrane, in the hypophase of the surfactant complex, on the plasmalemma of air-blood barrier cells and in its micropinocytosis vesicles, as well as on the membranes of osmophilic plate-like bodies as their contents egressed into the alveolar lumen.

Animals↗
PubMed1981

[Possibility of formation of "brush" cells from Type II alveolocytes in rats].

It was found that during acute alveolar hypertrophy (days 5-7 after lefthanded pneumonectomy) the number of alveolar brush cells considerably increases. Alveolocytes were disclosed to accumulate at that particular time in the epithelial lining of the alveoli. They consist of type II alveolocytes, brush cells and of intermediate-type alveolocytes. The latter ones were oval or cuboid in shape, had light cytoplasm with abundant polysomes, 1-4 osmiophilic lamellar bodies, as well as microfibrils and pinocytic vesicles. On the apical plasmalemma of the alveolocytes one could see the initial stages of the formation of cylindrical microvilli and microfibrils. The topography and ultrastructure of the intermediate-type alveolocytes may be viewed as evidence of the formation of the brush alveolocytes from type II alveolocytes.

Animals↗
PubMed1979

[Ultrastructural organization of the brush alveocytes of normal and hypertrophic rat lungs].

In non-inbred male rats, 140-200 g of body weight, the left lung was removed. In the right lung of the experimental and intact animals, brush alveolocytes were revealed electron microscopically and their structural organization was stated 1, 3, 7, 30 and 274 days after the operation. It was stated that during the process of compensatory hypertrophy in the lung, the occurrence rate of these cells increased considerably. In controls only 3 such cells were revealed, while in the experiment, the number of the brush alveolocytes was 20. In the experiment, most of the cells had signs of hyperfunction: increased amount of pinocytic vesicles in the apical part of the cell, dilated canaliculi in granular cytoplasmic network, formation of "laminar" structures from the membranes of the canaliculi of the granular cytoplasmic network. The brush alveolocytes were especially active during the period of acute alveolar hypertrophy (5-7 days after the operation) and correlated with the state of alveolar surfactant. The data obtained supported the hypothesis on chemoreceptor nature of the cells in question and demonstrated their participation in regulation of alveolar surface tension.

Animals↗
PubMed1978

[Light and electron microscopic study of rat lung brush alveolocytes].

The 52 alveolar brush cells (ABC) were revealed in the semi-thin sections of the rat lung tissue, metachromatically stained with toluidine blue. Characteristic features of the ABC on the light microscope level were the following: pyramidal body shape, basal position of the nucleus, darker stain tinge of the cytoplasm than that of other alveolar cells, the presence of microvilli on the small free cell surface. There is one ABC per 21 alveocytes, type 2, and 15 alveocytes, type 1. 41.1% of the ABC are localized in places of adjacent alveolus walls junction, 32.7%--on the alveolus wall facing the alveolus cavity, 16.8%--near the alveolus entrance; 9.4% of the cells are directed into the cavities of two neughbo ring alveoli or settle down near the pores of Kohn. In parallel electron microscopy there was revealed in ABC a form of granular cytoplasmic reticulum (unusual for other alveolocytes) in the form of blocks made up of 5-8 cysternae as if adherent one to another, bundles of filaments and microtubules, vacuoles in the apical cytoplasm. The ultrastructure of ABC, their topography, and incidence in the alveoli of rats evidenced their chemoreceptor nature.

Animals↗