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Biomedical subjects

L Pedersen

Publications and source records attributed to L Pedersen.

At least 127 records · Page 7Linked to original sources
PubMed1980

Isolation and characterization of a human liver and kidney-specific protein: the hepato-renal (H-R) antigen.

This paper reports the isolation and characterization of a soluble antigen shared by the liver and kidney of human and some other animal species. Homogenates of human liver in saline were centrifugated at 27,000 g and the supernatants were fractionated by preparative polyacrylamide gel electrophoresis. The gels were divided in sections and each was injected into rabbits; after absorption with polymerized normal human serum, the antiserum obtained by injecting one of the sections reacted only with saline extracts of human liver and kidney when tested against a variety of human tissue extracts. The absorbed antiserum, polymerized and insolubilized with glutaraldehyde, was used to purify the antigen by affinity chromatography. The purified antigen proved to be a glycoprotein containing 19 percent carbohydrate, had a molecular weight of 5.8-6.0 x 10(4) Daltons and a pI of 7.2-7.4. The antigen, relatively thermostable, was precipitated by 35-55 percent ammonium sulphate; its antigenic activity was not affected by extraction with 0.6 N perchloric acid or by incubation with ribonuclease, deoxyribonuclease or neuraminidase but was destroyed by incubation with ttypsin or chymotrypsin. Immunoperoxidase studies showed that the antigen appeared concentrated in the neclei of liver and kidney glomerular epithelial and tubular epithelial cells in humans and rats. The antigen could not be detected in human hepatomas or hypernephromas or in the rat Morris hepatoma 5123.

Adenocarcinoma↗
PubMed1980

Isolation and characterization of a human pancreas-specific protein.

Homogenates of human pancreas in saline were centrifuged at 27 000 X g and the supernates were fractionated by preparative polyacrylamide gel electrophoresis. The gels were divided into sections and each section was injected into rabbits; after absorption with polymerized serum from apparently normal humans, the antiserum obtained by injecting one of the sections was tested against a variety of human tissue extracts but reacted only with saline extracts of human pancreas. The absorbed antiserum, polymerized and made insoluble with glutaraldehyde, was used to purify a pancreas-specific antigen by immunoaffinity batch technique. The purified antigen proved to be a protein with some carbohydrate content (180 mg/g by weight) and a molecular mass of about 2.25 X 10(5) daltons. The antigen is relatively thermostable, and precipitates in the range of 245.64-340.2 g/L saturated ammonium sulfate; its antigenic activity is not affected by incubation with ribonuclease or deoxyribonuclease, but is destroyed by incubation with trypsin or neuraminidase and by extraction with perchloric acid. Immunofluorescence studies show that the antigen is diffusely present in the cytoplasm of pancreatic acinar cells.

Animals↗
PubMed1978

Long-term transfer-factor treatment for multiple sclerosis.

In groups of 16 patients with multiple sclerosis, 13 months' double-blind treatment with transfer factor from random normal donors differed from placebo treatment only in producing a temporary restoration of lymphocyte reactivity to measles virus antigen, and did not arrest the degeneration of nerve tissue.

Adult↗
PubMed1978

Computed tomography in hereditary ataxias.

Cranial CT in 39 patients (23 belonged to 8 families) with four different groups of hereditary ataxia (HA) showed mainly three combinations of atrophic findings: (1) cerebellar ataxia (CA, n = 17) had marked atrophy of the cerebellum and/or the brain stem combined with moderate cerebral atrophy; (2) an intermediate group consisting of hereditary spastic paraplegia (HSP, n = 10) and Friedreich's ataxia (FA, n = 7), both with moderate infra- and supratentorial atrophy; (3) atrophy was hardly demonstrated in the group of Charcot-Marie-Tooth disease (CMT, n = 5). HA cases with atrophy could be distinguished from multiple sclerosis (MS) by CT.

Adolescent↗
PubMed1978

Hematological response of rabbits to chronic, repetitive, severe bleedings for the production of antisera.

A study was undertaken to determine whether large amounts of blood (antiserum) could be rapidly removed from rabbits over a two-month period using a simple suction technique. It was found that half a blood volume could be readily removed each week (over 900 ml/rabbit/54 days) without a single fatality. The rabbits efficiently replaced the essential components of their blood during the experimental period as shown by a study of their blood parameters. They moderately overcompensated in replacing some of their blood components after a 6-week rest period. The study demonstrates that rabbits are satisfactory (in lieu of larger animals) for supplying relatively large amounts of antisera.

Anemia, Macrocytic↗
PubMed1978

An AB initio investigation of molecules with a disulfide bond: (HS)2, (CH3S)2 and cystine.

ab initio Calculations at the Gaussian-70 STO-3G and 4-31G basis levels have been carried out for (HS)2 and (CH3S)2. Cystine was investigated at the STO-3G level. The STO-3G energy minimized geometry agrees well with experiments for (HS)2 and (CH3S)2. The barriers to internal rotation are predicted to be (at the 4.31G level): (HS)2, cis 8.5 kcal, trans 3.03 kcal; (CH3S)2, cis 18.47 kcal, trans 6.04 kcal.

Chemical Phenomena↗
PubMed1978

Computerized tomography in hereditary ataxias.

Thirty-nine patients with hereditary ataxia (HA), hereditary spastic paraplegia or Charcot-Marie-Tooth disease were investigated with computerized cranial tomography (CT). Infratentorial as well as supratentorial atrophies were registrated and scored. These were compared with the patient's neurological symptoms, which were related to the lesion in the central nervous system, and scored. There was correlation between the distribution of brainstem/cerebellar symptoms and the distribution and degree of infratentorial atrophy. HA cases with cerebral cortical atrophy had significantly higher dementia scores than those without wide sulci. Intravenous injection with contrast medium was of no diagnostic aid, as no focal changes were found. It is concluded that CT is an aid in the diagnosis of hereditary ataxias and hereditary spastic paraplegia.

Adult↗
PubMed1978

Effects of oral phenylalanine load on plasma glucagon, insulin, amino acid and glucose concentrations in man.

An oral phenylalanine load provokes a significant drop in serum tyrosine levels in children with phenylketonuria [8]. The aim of the present investigation was to examine the response of insulin and glucagon to oral phenylalanine loading as these hormones are known to have a hypoaminoacidaemic effect. Six adult normal weight and healthy men were loaded orally with 0.6 mmol L-phenylalanine per kg body weight after an overnight fast. Serum phenylalanine increased within 10 min after the load and reached a maximum concentration at 30 min. Serum tyrosine increased within 10 min after the load and reached a maximum concentration at 2 h. Plasma glucagon and insulin increased during the first 10 min after the load and reached a peak twice the fasting levels at 30 min after the load. The molar insulin/glucagon ratio remained unchanged during the first 20 min after the load but then declined by 50% at 2 h. Associated with this decline plasma amino acid concentration (except phenylalanine and tyrosine) declined by approximately 15%. The decline was most marked for isoleucine, leucine, methionine and valine. As the hypoaminoacidaemic effect of insulin and glucagon is known to be most marked for these four amino acids plus phenylalanine and tyrosine, the response of insulin and glucagon to a phenylalanine load may influence not only the fate of phenylalanine given but also the blood tyrosine level.

Administration, Oral↗
PubMed1977

Insoluble human plasma protein immunoadsorbents. Large-scale production and storage.

Methods for producing and preserving large volumes of insoluble immunoadsorbents (for removing unwated antibodies to serum proteins) from surplus blood bank plasma by glutaraldehyde were evaluated by quantitative and qualitative means using radioactive 125I and immunoelectrophoresis, respectively. Some of the factors affecting the desired physical characteristics and antibody-absorbing properties of the imjunoadsorbent studied were: plasma acidification, varying concentrations of glutaraldehyde, addition of small amounts of formalin, storage under varying conditions of temperature, and exposure to preservatives in the wet and lyophilized state for periods up to 2.5 years. The best preservation of antibody-adsorbing properties (under storage conditions) was obtained in the washed state at 4 degrees C, but good preservation was also obtained at room temperature in the presence 10% formalin and in the unwashed state at room temperature in the presence of unreacted glutaraldehyde. Lyophilization destroyed about 70% of an adsorbent's activity.

Animals↗
PubMed1977

HLA (SD and LD) in patients with amyotrophic lateral sclerosis (ALS).

Twenty-five patients with a definite diagnosis of amyotrophic lateral sclerosis (ALS) were HLA-typed for the serologically detectable antigens A, B and C and MLC-typed for 7 HLA-D-determinants. No significant deviation was found neither in the A, B, and C-series nor in the HLA-D-series as compared to normal controls. The aetiological problem of ALS is discussed.

Adult↗
PubMed1977

Endurance test as index of paresis in patients with multiple sclerosis.

This study has been performed to evaluate slight pareses in patients with multiple sclerosis by means of mechanical endurance experiments. The results are compared with manual grading of pareses in patients and with healthy controls. On average, concordance was found between manual and mechanical estimation but the method is not advantageous for clinical purposes.

Humans↗
PubMed1977

Reflex dystrophy. Resting blood flow and muscle temperatures as diagnostic criteria.

Fifty-one patients were examined following an injury of the upper extremity. Clinically, 25 patients were estimated as having reflex dystrophy, while the rest was categorized as either having a possible reflex dystrophy or acted as controls. Temperature and blood flow measurements were carried out on both extremities of the patients. The temperature of the brachioradial muscle and resting blood flow of the same segment of the forearm gave highly significant elevated values on the reflex dystrophic arms as compared with the healthy side, while the control patients revealed no such differences. Combination of these two parameters proved to give a specificity and sensitivity that high that they can be recommended as a diagnostic tool. A quantitative measurement and exact diagnosis make controlled trials on this group of patients easier in the future.

Arm Injuries↗
PubMed1976

Idiopathic bile acid catharsis.

In the course of extensive routine screening for bile acid malabsorption a few patients were detected in whom chronic diarrhoea was apparently induced by excess bile acid loss which was neither associated with demonstrable conventional ileopathy nor with any other disorder allied to diarrhoea. In three patients subjected to scrutiny the results obtained were in harmony with a concept of idiopathic bile acid catharsis. Ingestion of cholestyramine was followed by immediate relief, but the diarrhoea recurred whenever this treatment was withdrawn. It it suggested that idiopathic bile acid catharsis should be suspected in patients with unexplained chronic diarrhoea and especially in those with a diagnosis of irritable colon with diarrhoea.

Adult↗