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L Pospísil

Publications and source records attributed to L Pospísil.

At least 19 recordsLinked to original sources
PubMed1997

[Occurrence of antibodies to chlamydial group antigens in the population of the Czech Republic].

A set of 4,178 human blood sera was tested including 3,093 samples collected from blood donors, 624 randomly collected samples. 124 samples collected from persons possibly exposed to Chlamydia infection, and 337 samples from patients of various hospitals in the districts of Brno, Olomouc and Rumburk. All the sera were examined using the complement fixation microtest and the antigen of Chlamydia psittaci for CFR (Bioveta, Ivanovice na Hané, CzR). Moreover, a subset of samples collected from selected groups (n = 209) was tested by CFR using the genus-specific Ornithose-Antigen (Behring, Germany) (n = 209), another subset of 116 samples by indirect immunofluorescence using the biological Chlamydia trachomatis Spot IF (Bio Merieux) (n = 116), and a subset of 224 samples by ELISA for the demonstration of IgG, IgM and IgA class of antibodies using the kit rELISA Medac. Complement fixing antibodies assayed with the Chlamydia psittaci antigen (Bioveta), reached titres of 1.5, 1:10 and 1:20 in 15.5%, 5.29% and 0.48% of the randomly collected samples, respectively. The corresponding values for the tests carried out with the Ornithose-Antigen were 1:10 in 10.9%, 1:20 in 8.9%, 1:40 in 2.9% and 1:80 in 1.4%. ELISA yielded 52.7%, 26.85% and 17.59% positive results in the globulin classes IgG, IgA and IgM, respectively. Antibody titres from 1:20 and from 1:10, determined with the Bioveta antigen, were found in 2.36% and 9.47% within a representative group of blood donors. The corresponding values for the group of the hospitalized patients were 14.54% and 24.93%, respectively, and for the group of persons possibly exposed to Chlamydia infection 2.42% and 8.87% respectively. The indirect immunofluorescence test yielded positive, negative ad doubtful results in 47.41%, 27.59% and 25.0% persons of the latter group. This group showed also a higher percentage (37.07%) of positive results in ELISA in the class IgA than the randomly selected persons.

Antibodies, Bacterial↗
PubMed1996

[Detection of chlamydiae in animal and human semen using direct immunofluorescence].

Frequency of elementary and reticular chlamydial bodies was investigated by direct immunofluorescence tests in ejaculates collected from 52 men, 60 stallions, 42 bulls, and 66 boars using the kits of Progen Biotechnic GmbH and the microscope Labophot-2 Nikon. At the same time, qualitative semen tests, including ejaculate volume, sperm motility, percentage of live and dead sperms and morphological' analyses (Vĕzník and Svecová, 1992) were done. Repeatability of the findings was checked in a group of nine bulls housed at the institute and sampled at weekly intervals for 3 to 4 months (Tab. 1). Chlamydiae were demonstrated in 3.8%, 14.3%, 3.4% and 9.1% of the human, bull, stallion and boar ejaculates, respectively (Fig. 1). A relation between the presence of Chlamydiac and impaired functional and morphological quality of ejaculates was found in contaminated human (only two samples) and bull ejaculates (Fig. 2) and in one of the two positive stallion ejaculates (Tab. II, Fig. 3). Direct tests for Chlamydiae should be included into semen health and quality checks as one of the major tasks of assisted reproduction in human and veterinary medicine.

Animals↗
PubMed1996

[Detection of Chlamydia in inflammation of the cervix and uterus in cows].

Clinical findings in cows of seven farms with a frequent occurrence of birth canal inflammations were matched against results of serological and direct immunofluorescence tests for Chlamydia sp. infection. Normal clinical picture, weak to moderate and serious inflammations were found in 16.1%, 46.7% and 37.2% of the 205 examined cows, respectively. The results of micro CFT were negative in 32.7% and titres 1:5, 1:10, 1:20, and 1: > or = 40 were found in 8.7%, 38.0%, 12.3%, and 8.3%, respectively. Direct immunofluorescence test of cervical swabs, using the Progen Biotechnic GmbH set, was negative in 29.1% and clearly positive in 34.7% of the tested cows. The rest was animals in which sporadic elementary bodies were seen. The significance of the findings is discussed and the necessity to test Chlamydia infections in herds affected by frequent and/or chronic inflammations of genital organs is stressed. Evidence of the transmission of Chlamydia infection by contaminated semen is presented.

Animals↗
PubMed1996

[Detection of chlamydia in the intestines and lungs in pigeons and humans].

Attempts were made to detect Chlamydia sp. by direct immunofluorescence (IF) in intestinal and lung tissues collected from wild town pigeons, young reared pigeons, and human corpses. Chlamydia in any organ were detected in 86.6% of the 427 adult pigeons and 27.3% of 16 young pigeons. Positive IF results were in intestinal and lung samples, collected from 427 adult pigeons, in 34.3% and 40.05% of the birds, respectively. Human (n = 100) intestinal and lung tissues were positive in 22% and 20% of the corpses, respectively. Titres 1:20 and higher were demonstrated by CF in 53.86% of the 427 adult pigeons. The highest titre found was 1:5 120.

Adult↗
PubMed1993

Antigenicity of Dermatophilus congolensis hemolysin.

The separated cell-free form of hemolytic exosubstance was obtained from five strains of Dermatophilus congolensis. Three strains produced exosubstance with high activity, two strains produced exosubstance with lower intensity of activity. The separated forms exhibited the same hemolytic interactions as the native forms produced by growing strains, namely the antagonism with staphylococcal beta hemolysin and the synergism with staphylococcal delta hemolysin, streptococcal CAMP factor and rhodococcal equi factor. Rabbit sera obtained after intravenous or intraperitoneal application of the separated forms contained precipitation and neutralization antibodies. Cross tests of precipitation and neutralization proved antigen identity of hemolysins of different D. congolensis, strains which makes the serodiagnostics of this species possible.

Actinomycetales↗
PubMed1992

Hemolytic interactions of Dermatophilus congolensis.

The strains of Dermatophilus congolensis grew on blood agar with washed sheep erythrocytes with marked total hemolysis. In testing for hemolytic interactions they gave a significant synergistic effect of a characteristic shape with Rhodococcus equi and Streptococcus agalactiae, whereas with Staphylococcus aureus producing beta hemolysin and with Staphylococcus aureus producing delta hemolysin a simultaneous synergistic as well as antagonistic effect were observed. First of all a conspicuous inhibition of in the beta hemolysin zone began and then the hemolytic effect of D. congolensis was enhanced. A similar double reaction was also observed with Listeria ivanovii. With delta hemolysin there was an inhibition of the hemolytic effect of D. congolensis and at the same time a synergistic effect could be observed. Also D. congolensis gave a weak synergistic effect with Micrococcus lylae and Listeria monocytogenes, and a further weak antagonistic effect with alpha hemolysin of Staphylococcus aureus, Staphylococcus hyicus, Staphylococcus chromogenes and Micrococcus luteus. No interaction of D. congolensis was established with Corynebacterium pseudotuberculosis.

Actinomycetales↗
PubMed1992

Experimental dermatophilosis.

The histopathologic features of an experimental dermatophilosis of rabbit skin were described. The strain of Dermatophilus congolensis used for the experimental infection was obtained from the unique isolation and cultivation of this actinomycete from the ulcerous leg lesion of one male patient. Further strains in experiment came from the collections of type cultures. The experimental infection was characterized as an exudative and crustal dermatitis with acanthosis and subcorneal abscesses. Branching hyphae and clusters of coccoid bodies were found mostly in the parakeratotic layer and infrequently in the abscesses. Both the coccoid bodies and the hyphae were Gram-positive. The hyphae were acid-fast but the coccoid bodies were non-acid-fast. The fine structure of hyphae and coccoid bodies, namely of those with flagella, was also described by means of a transmission and scanning electron microscope.

Actinomycetales↗
PubMed1990

Corneal phaeohyphomycosis caused by Wangiella dermatitidis.

A case is described of a corneal abscess of the left eye of a patient, male, 35 years, from which there was found in pure culture a mycotic micro-organism, Exophiala (Wangiella) dermatitidis, determined also in the native preparation from the pus. The patient is feeble-minded, suffers from von Recklinghausen's disease and has an impairment of the immune system (above all the reduction of T lymphocytes).

Abscess↗
PubMed1989

[Typing of skin staphylococci using the commercial identification system Staphy-Test].

The authors examined 150 strains of S. aureus and 180 strains of S. epidermidis isolated from different dermatoses. The determination of S. aureus and S. epidermidis was based on common routine tests as plasmacoagulase, production of hemolysin, acid production from mannitol and production of pigment. This determination was verified with the aid of a new identification system STAPHYtest. The STAPHYtest confirmed the previous diagnosis of S. aureus in 60.66% and that of S. epidermidis in 31.66% of strains only. The remaining strains were identified by means of STAPHYtest as S. simulans, S. xylosus, S. hominis, S. haemolyticus, S. warneri, S. auricularis, S. sciuri and Micrococcus sp. The authors discuss the importance of an curate identification of skin staphylococci by means of STAPHYtest for taxonomic purposes and for classification of staphylococci influence on different dermatoses.

Bacteriological Techniques↗
PubMed1983

A simplified ELISA method for syphilis.

A new serological method for syphilis using a modified ELISA reaction is given. The reaction is performed on polystyrene carriers which have absorbed an ultrasonicated preparation of T. pallidum. The reaction between the antigen and antibody is detected by means of the addition of antihuman IgG swine globulin conjugated with horseradish peroxidase. To make the reaction visible, addition of 5-methyl-9-aminocarbazol in dimethylformamide is necessary. In a positive case of syphilis the carrier becomes a red-brown-violet color; in a negative reaction the white color does not change. The results obtained with this reaction are in absolute concordance with those of FTA-ABS and TPHA.

Antigens, Bacterial↗
PubMed1983

Further observations of Corynebacterium renale as an indicator organism in the detection of the exfoliation-positive strains of Staphylococcus aureus.

Exfoliatin-positive S. aureus strains of known phenotypes were examined for bacteriocin production using S. aureus 209P, C. pseudodiphtheriticum, or C. renale as indicator strains. Strains of the phenotype Bac+ Tox+ had a positive result when the simultaneous method was used, and produced a wide inhibition of the indicator strains, especially of C. renale, if the deferred method was used. The strains of the phenotype Bac- Tox+ were negative upon examination by the simultaneous method, but they caused narrow inhibition zones of the indicator strains to appear with the deferred method, especially with C. renale. S. aureus strains of diverse origin used as controls were in both methods negative with regard to inhibition of indicator strains. C. renale was found to be very suitable for the detection of bacteriocins of the exfoliatin-positive staphylococci, particularly when using the deferred method.

Bacterial Toxins↗