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L S Rao

Publications and source records attributed to L S Rao.

4 recordsLinked to original sources
PubMed1990

A mammary stimulating mitogen(s) from rat muscle: its possible influence in mammary tumorigenesis.

A mitogen has been shown to be present in rat abdominal muscle tissue, which could stimulate rat mammary cells grown in culture. Studies on the in vivo-stimulatory effects on the rat mammary gland, taking increase in DNA synthesis, mitotic index and wholemount preparation as the parameters, affirmed the presence of a mammary stimulating factor in the muscle tissue. Treatment of rat mammary glands with the crude mitogen preparation before and after a single gastric dose of 9,10-dimethyl-1,2-benzanthracene (DMBA), increased the mammary tumor incidence suggesting its possible influence in carcinogen-induced tumorigenesis in rats.

9,10-Dimethyl-1,2-benzanthracene↗
PubMed1989

A mitogenic factor from rat muscle.

A growth-promoting polypeptide has been purified about 1950-fold from rat abdominal muscle by gel chromatography, isoelectric-focusing (IEF) and reverse phase high pressure liquid chromatographic (RP-HPLC) techniques. This factor is an acidic protein with an isoelectric point (pI) of 3.4 and a mol. wt of 12 kDa when run on SDS-polyacrylamide gels under reducing conditions. It is acid and heat-stable but is sensitive to reducing agent and protease action. It stimulates [3H]thymidine incorporation in NRK-49F and NIH 3T3 cells and induces soft agar colony formation of NIH 3T3 cells. The muscle-derived mitogen appears to differ from other known growth factors (GFs) in its physico-chemical properties.

Animals↗