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A mitogenic factor from rat muscle.

L S Rao·S G Rao·A P Banerji
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Abstract

A growth-promoting polypeptide has been purified about 1950-fold from rat abdominal muscle by gel chromatography, isoelectric-focusing (IEF) and reverse phase high pressure liquid chromatographic (RP-HPLC) techniques. This factor is an acidic protein with an isoelectric point (pI) of 3.4 and a mol. wt of 12 kDa when run on SDS-polyacrylamide gels under reducing conditions. It is acid and heat-stable but is sensitive to reducing agent and protease action. It stimulates [3H]thymidine incorporation in NRK-49F and NIH 3T3 cells and induces soft agar colony formation of NIH 3T3 cells. The muscle-derived mitogen appears to differ from other known growth factors (GFs) in its physico-chemical properties.

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AnimalsBinding, CompetitiveCell LineCell Transformation, NeoplasticCells, CulturedChromatography, GelChromatography, High Pressure LiquidDNA ReplicationErbB ReceptorsFemaleIsoelectric FocusingMitogensMolecular WeightMusclesProteinsRatsRats, Inbred StrainsJournal Article

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L S Rao, S G Rao, A P Banerji. 1989. A mitogenic factor from rat muscle.. https://doi.org/10.1016/0304-3835(89)90032-3

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