A hybrid vaccinia virus expressing a chimeric protein consisting of thymidine kinase and the encephalitogenic determinant, S1, from guinea pig myelin basic protein was constructed. Infection of guinea pigs with the virus resulted in the development of allergic encephalomyelitis.

Animals↗

PubMed1991

[A chemically synthesized gene assures the biosynthesis of a polypeptide in Escherichia coli cells, the structure of which corresponds to human leukocytic interferon alpha 2].

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Amino Acid Sequence↗

PubMed1991

[Molecular mechanisms of selecting influenza virus strains by signs of drug resistance].

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Amino Acid Sequence↗

PubMed1991

[Variants of the Venezuelan equine encephalomyelitis virus resistant to the neutralizing action of monoclonal antibodies].

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Amino Acids↗

PubMed1991

[Marburg virus: the first determined nucleotide sequence of two genes].

The preparations of purified Marburg virus were isolated from blood plasma of infected guinea pigs and characterized. Viral RNA was extracted from the virions. The cDNA was synthesized on the isolated RNA matrix by the reverse transcriptase with the use of dissipated priming. The obtained cDNA was inserted into the plasmid pBR322 by the connector technique and the resulting recombinant plasmids were cloned in Escherichia coli cells. The specific clones selected by molecular hybridization method were analyzed by the restriction mapping and cross-hybridization. Four overlapping cDNA clones were found and the virus specific 5012 bp fragment of the viral genome was sequenced. Three open reading frames were found and the preliminary analysis of the coded amino acid sequence and corresponding genes was fulfilled.

Amino Acid Sequence↗

PubMed1991

[Nucleotide sequence of genes and complete amino acid sequence of tick-borne encephalitis virus strain 205].

The 10466 nucleotide long sequence of the cDNA copy of the tick-borne encephalitis strain 205 viral genome has been determined. It includes the 5'-nontranslating region, the genes for structural as well as nonstructural proteins and the first 93 nucleotides of 3'-nontranslating region. The difference in amino acid sequences of structural and nonstructural proteins of strains 205. Sofjin and Neudoerfl of the tick-borne encephalitis virus and the nucleotide changes in 5'- and 3'-nontranslating of these strains are discussed.

Amino Acid Sequence↗

PubMed1991