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L Sobotka

Publications and source records attributed to L Sobotka.

47 records · Page 3Linked to original sources
PubMed1980

Influence of antithymocytic serum on the regenerating activity of the liver after partial hepatectomy in mice.

In experiments on mice the effect of rabbit-anti-mouse antithymocytic serum on the growth of the liver and on the onset of proliferating activity of the liver after partial hepatectomy was studied to elucidate the question of assumed participation of the lymphoid system in the proliferation of hepatic tissue. Administration of three doses of antithymocytic serum (0.1 ml/10 g) by the i.p. route in 24 hour intervals led to a marked increase of the weight of the spleen and increase of DNA synthesis in liver of non-operated mice. Administration of serum 24 hours before partial hepatectomy (PHE) concomitantly with PHE and 24 hours after PHE did not influence substantially the rate of DNA synthesis in the regenerating liver 34 and 48 hours after PHE and did not influence the mitotic index of hepatocytes. In the livers of mice to whom antithymocytic serum was administered proliferation of cells of the lymphocyte series and phagocyte activation occurred. These cells were located in the hepatic sinuses and periportal areas. Antithymocytic serum--contrary to chemical immunosuppressive substances--does not exert an inhibitory action on regeneration of hepatic tissue; alone does not even cause inhibition of the lymphoid system which could exert a negative effect on regeneration of the liver.

Animals↗
PubMedDate not supplied

Influence of rapid amino acid and lipid emulsion administration on gas exchange and resting energy expenditure.

The aim of this study was to observe the effect of rapid lipid and amino acid infusion on resting energy expenditure (REE) in patients on parenteral nutrition. Eight people were studied. After 8 h of saline administration, they received 20% lipid emulsion and 8% amino acid solution during the next 5 h (rate 3.8 mg.kg-1.min-1 fat and 1.9 mg.kg-1.min-1 amino acid). Before the onset of the infusion and 3 and 5 h after, gas exchange and REE were measured. The rapid lipid emulsion administration led to a significant rise in serum triglyceride level. A significant increase in VO2 (from 269.0 +/- 66.8 to 319 +/- 55.8 ml/min) and REE (from 1858 +/- 438 to 2155 +/- 385 kcal/24 h) was observed 5 h after the onset of substrate administration (P less than 0.01). Pulmonary ventilation and VCO2 production were not influenced by the infusion. The thermic effect of nutrients administered reached 3.5 and 6.5% 3 and 5 h after the start of infusion, respectively. We conclude that REE can be increased by the rapid administration of lipid-based parenteral nutrition.

Adult↗