PubMed Health⌕ Search

Biomedical subjects

L Syrůcek

Publications and source records attributed to L Syrůcek.

At least 19 recordsLinked to original sources
PubMed1990

[Microbiological diagnosis of infection with the human immunodeficiency virus].

The author describes and evaluates different approaches in laboratory diagnosis of HIV infection and AIDS. Serological detection of anti-HIV antibodies provides despite some limitations very good diagnostic opportunities. ELISA screening tests supplemented by confirming tests, in particular the Western blot, ensure maximum accuracy of the obtained results. Evidence of the viral antigen in serum is used as a diagnostic criterium only in special instances and serves rather for monitoring of the state of HIV infected subjects than screening for new cases of infection. Cultivation of the virus from clinical material is difficult but in some instances, e.g. in neonates and young children it is the only evidence of HIV infection. Recently for diagnostic purposes molecular biological methods are used, in particular amplification of proviral DNA by a polymerase chain reaction. Their application is, however, so far restricted to research departments because they are technically and economically pretentious.

AIDS Serodiagnosis↗
PubMed1990

[Dynamics of the antibody response and p24 antigenemia in HIV-1 infected individuals in Czechoslovakia].

In a group of 83 anti HIV-1 positive subjects the antibody response against different structural viral proteins was investigated concurrently with assessment of p 24 antigenaemia. Disappearance of antibodies against the capsidal antigen p 24 was recorded in 12% of 42 patients with ARC and AIDS. On the other hand, these antibodies persisted in all 41 asymptomatic infections throughout the three-year investigation period. Disappearance of antibodies against p 17 antigen was proved in 41% of the patients and in 22 subjects without clinical symptoms. The authors found a rising trend of antigenaemia p 24 in the course of HIV infection. The free p 24 antigen was detected in 62% patients with AIDS and only in 10% asymptomatic infections. The investigation confirmed the importance of investigations of the antibody response and p 24 antigenaemia in the prognosis of development of HIV infection and in monitoring of the effect of therapy.

AIDS-Related Complex↗
PubMed1990

[Detection of HIV infection using the immunofluorescence test].

The author examined, using the immunofluorescence test, 107 ant HIV positive sera and 114 sera with false reactions in the ELISA test. He proved a high sensitivity and specificity of the immunofluorescence test in the detection of anti-HIV antibodies, comparable with Western-blot's technique and he established the value of the immunofluorescence test for the identification of the virus antigen on isolation of the virus from HIV infected patients.

AIDS Serodiagnosis↗
PubMed1990

[Cultivation of HIV-1 virus on mononuclear peripheral blood cells of HIV-1 seropositive individuals].

Using the method of co-cultivation with phytohaemagglutinin-stimulated lymphocytes from healthy donors, the author isolated the HIV-1 virus from peripheral mononuclear blood cells of three patients with the AIDS symptomatology and one patient with the ARC symptomatology. The presence of the virus in infected cells was proved by detection of the viral antigen p 24 in enzymatic immunoassays and in the immunofluorescence test. Three of the isolated strains were adapted to sensitive continual tissue cultures, where the isolates caused chronic infection of the cells associated with the development of a cytopathic effect. In the investigated patients no relationship was proved between viraemia and antigenaemia. The author discusses the importance of virus cultivation for laboratory diagnosis, epidemiology and research of HIV infection and AIDS.

AIDS-Related Complex↗
PubMed1989

[Childhood AIDS].

The authors emphasize the present importance of AIDS in children and explain the causes of the rising trend of this syndrome in the child population. They summarize the most important epidemiological characteristics of AIDS in children, its pathogenetic differences and the ensuing clinical symptoms. Attention is drawn to the pitfalls of microbiological diagnosis of child infections caused by HIV, and the authors discuss principles, possibilities and perspectives of causal treatment of this syndrome in children. Finally they summarize contemporary experience and knowledge regarding immunization of children infected with the human immunodeficiency virus.

Acquired Immunodeficiency Syndrome↗
PubMed1989

Occurrence and aetiology of acute respiratory diseases: results of a longterm surveillance programme.

Totals of 58,661,000 acute respiratory disease (ARD) cases, 1,376,651 bronchitis and pneumonia complications, and 93,042 deaths from influenza, bronchitis, pneumonia or chronic pulmonary affection were notified during 11 years of ARD surveillance from 1975 to 1986. All ARD seasons started with the first phase in September-December; this increase in morbidity was caused chiefly by adenoviruses, parainfluenza viruses, rhinoviruses and M. pneumoniae. Second wave of ARD morbidity occurring in January-April used to be explosive and was associated with an influenza epidemic in 9 of the 11 seasons; only in 1978/79 and 1984/85 the ARD epidemics were caused by adenoviruses and especially RSV, the share of influenza being minimal. Pneumonia and bronchitis excesses occured during epidemics caused by M. pneumoniae in 1975/76, 1980/81 and 1985/86. Particularly high mortality excesses occurred in 1976, 1977 and 1983 during epidemics elicited by a new drift variants of influenza A(H3N2). Identification of viral agent of M. pneumoniae attempted in 5474 ARD cases was successful at 37.4%. The respective contributions of parainfluenza viruses, adenoviruses, influenza A virus and RSV to overall aetiologically identified morbidity were 14.2, 13.9, 13.8, and 12.0%. Mixed infections (2-3 agents identified simultaneously) accounted for 14.6% of cases. Type B influenza virus, rhinoviruses, enteroviruses and herpes simplex virus contributed only by 5.6-7.8%. In ordinary seasons the share of M. pneumoniae in aetiologically identified ARD morbidity was 0.6-3.8%; this agent displayed predominance at 5-year cycles, when accounting for 20.5-38.9% of cases. The most frequently detected agents in individual age groups were as follows: in preschool children parainfluenza (18.6%), RSV (16.6%), and adenoviruses (17.4%); in school children M. pneumoniae (26%), influenza A and B (10.2 and 14.7% respectively), and adenoviruses (10.7%); in adolescents and young adults influenza type A (20.2%), M. pneumoniae (15.0%), and rhinoviruses (13.3%); in adults above 25 years age influenza A virus (38%), and other respiratory viruses at a frequency lower than 10% each.

Adolescent↗
PubMed1989

Epidemiological analysis of acute respiratory diseases (ARD) of viral etiology in the Czech Socialist Republic (CSR)) and German Democratic Republic (GDR) over the period 1979 to 1984.

An analysis is made of the ARD reported in CSR and the GDR over the period July 1st, 1979 to June 30th, 1984. During that time, there were 27,810,000 cases reported in CSR in the framework of ARD epidemiological surveillance, representing 2.67 cases per one inhabitant, whereas in the GDR, the total number of reported ARD was 28,900,000 yielding 1.73 cases per person. However, the GDR reported higher morbidity per one child of preschool age. The authors believe that the differences in the reported incidence of ARD between the two countries are due to differences in the reporting systems and medical officers' activity during an epidemic and in the interim period. Approximately one third of ARD reported annually in the two countries falls to the period of influenza epidemics. The authors also analyze the etiology of the influenza epidemics which affected the two countries in 1980, 1981, 1982, 1983 and 1984. In most seasons, the causative agents and morbidity excesses were different in the two countries. The drift variant B/USSR/100/83, which caused a major epidemy in CSR in 1984, has not to date been implicated in the DGR in the etiology of ARD. The cyclic epidemic due to Mycoplasma pneumoniae occurred in the GDR already in 1979-80, while CSR experienced it a year later. There was a temporal and territorial correlation between the course of A(H1N1) influenza epidemic in the two countries in 1984.

Acute Disease↗
PubMed1989

Immunogenicity of a chromatographically concentrated influenza vaccine.

The immunogenicity of the Czechoslovak-made commercial vaccine Adinvira as well as that of the chromatographically concentrated vaccine Leningrad of Soviet origin was evaluated in an immunization project. The both vaccines were well tolerated after administration, the Soviet-made Leningrad brand featuring greater efficiency in eliciting an antibody response against influenza virus haemagglutinin.

Antibodies, Viral↗
PubMed1986

Influenza activity in Czechoslovakia and the USSR, 1980-1985.

Between 1980 and 1985, Czechoslovakia had experienced 4 and the USSR 3 major influenza outbreaks. Of the 3 epidemic outbreaks in the USSR, 2 were associated with influenza B virus (in the 1980/81 and 1983/84 seasons) and 1 with influenza A virus of the H3N2 subtype. In the USSR, influenza A (H1N1) virus never predominated as a cause of epidemic during the 5 years period. In Czechoslovakia, 2 epidemics (in the 1980/81 and 1983/84 seasons) were due to influenza A (H1N1) virus. The epidemic in the 1981/82 season had two waves of unequal heights and a mixed type B and subtype A (H3N2) etiology; a two-wave epidemic associated with isolates of influenza A (H1N1) and influenza B viruses was also recorded in the 1983/84 season. The influenza A (H3N2) epidemic in 1983 was of explosive character. All influenza viruses circulating in the two countries between 1980 and 1985 were of the same antigenic profile, but were isolated from the epidemics that occurred in different influenza seasons. The virological surveillance revealed strains of virus closely related to drift variants detected from outbreaks in 1977-1979 and the new variants A/Chile 1/83, A/Philippines 2/82, A/Caen 1/84 and B/USSR 100/83.

Czechoslovakia↗