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Biomedical subjects

L Syrůcek

Publications and source records attributed to L Syrůcek.

At least 37 records · Page 2Linked to original sources
PubMed1981

Detection of respiratory syncytial virus serum antibodies by an ELISA system.

An ELISA test for respiratory syncytial (RS) virus assay was adapted and standardized; it gave 10-15 times higher antibody titres than complement fixation (CF) but was not a more sensitive test for detecting recent RS virus infection in persons above 1 year of age. In testing normal-population sers, ELISA revealed twice more positive sera than the CF test. Owing to its high sensitivity and apparent ability to detect long-persisting antibodies, ELISA is the test of choice for sero-epidemiological surveys on RS virus infections.

Acute Disease↗
PubMed1980

An outbreak of respiratory disease due to a type 5 adenovirus identified as genome type 5a.

A cluster of adenovirus type 5-caused acute respiratory diseases was observed in Prague in the autumn-winter season of 1978/79. An analysis of viral DNA by sequence-specific restriction enzymes in two out of 39 isolates revealed that these isolates, identical to each other, were clearly distinct from adenovirus type 5 prototype strain. The designation "adenovirus genome type 5a" is suggested for these isolates.

Adenoviridae Infections↗
PubMed1980

Neurological diseases associated with viral and Mycoplasma pneumoniae infections.

In 1963 the World Health Organization established a system for the collection and dissemination of information on viral infections and by 1976, laboratories in 49 countries were participating in this scheme. The present study is in two parts: part 1 is an analysis of almost 60 000 reports on neurological disease associated with viral and Mycoplasma pneumoniae infections reported during the 10-year period 1967-76. This analysis showed a steady increase in the yearly number of reports of viral neurological diseases, which closely followed the general increase in the overall reporting of virus diseases. Likewise, the seasonal pattern was similar to that seen in general for any given virus.Over 75% of the cases were in children. Over half of all viral neurological diseases were associated with enteroviruses, while the myxoviruses accounted for almost 30%. Among the myxoviruses, mumps virus was by far the most frequently reported. The polioviruses were the agents most commonly detected in cases of paralytic disease. The other enteroviruses, mumps virus, and the herpesviruses were the most frequently reported viruses in cases of aseptic meningitis or encephalitis. On the other hand, one-third to over one-half of the reports on the myxoviruses (excluding mumps and measles) related to ill-defined clinical conditions.Part 2 of the study deals in particular with viruses whose role in neurological disease is less well documented. One laboratory reported an outbreak of adenoviral aseptic meningitis in Czechoslovakia, while another described neurological disease associated with M. pneumoniae infection in Finland. Part 2 also includes a detailed appraisal of viral infections diagnosed in the Netherlands during the period 1973-76. The results are very similar to those routinely reported.

Enterovirus Infections↗
PubMed1979

The spread of rhinoviruses in the Soviet Union (USSR) and in Czechoslovakia (CSSR) and the role of their pathology.

Virological, serological and immunofluorescence studies revealed circulation of rhinoviruses of the strains 1A, 1B, 2, 3, 7, 9, 10, 12, 13, 14, 16-23, 27, 29-33, 42, 48, 53, 56, 60 and 69 on the territory of Czechoslovakia and the Soviet Union. According to virological results, type 48 predominated and was followed in frequency of occurrence by types 27, 14 and 16 in the USSR and 30, 1A and 31 in the CSSR. RV infection in adults with ARD diseases was the aetiology in 28.5% of cases. Among the causative agents of acute rhinitis, rhinosinusitis and otitis, rhinovirus infection makes up a large proportion. Interrelation has been found between rhinovirus infection and chronic inflammation of the mucosa of the nose, the accessory sinuses and the ears. Prolonged course of rhinovirus infection and isolation of the virus in the late stages of disease (1-4 months after its onset) support the presumption that rhinoviruses promote chronicity of inflammation. RV was isolated from patients with chronic pneumonia from the lower respiratory passages (bronchial secretion). Rhinoviraemia was found in a child who died of ARD.

Adolescent↗
PubMed1978

Haemagglutination inhibition antibodies in nasal secretions of persons after natural parainfluenza virus infection.

In 7 adults and 7 children with upper respiratory illness of parainfluenza aetiology, the virus was isolated in the acute phase from nasal washings or nasal smears in spite of high titres of haemagglutination inhibition (HI) antibodies in serum. By contrast, secretory HI antibodies were not demonstrated at the onset of illness in any of the patients, but their formation started early and the antibodies reached maximal levels about 10 days after onset of illness. The individual patients showed considerable differences in the dynamics of secretory antibody formation and especially in their persistence. In some of the patients, secretory antibodies were demonstrated as late as 12 months after the illness.

Adolescent↗
PubMed1976

Levels of immunoglobulins and antibodies to haemaglutinin and neuraminidase of influenza virus in nasal secretions after natural infection.

Nasal washings (NW) from 16 influenza patients in the course of an epidemic in November and December, 1974 were examined for the presence of influenza virus, immunoglobulins (Ig) and titres of haemagglutination inhibiting (HI) and neuraminidase inhibiting (NI) antibodies. Influenza virus identical with A/Port Chalmers/1/73 (H3N2), increased levels of IgA and occasionally IgG, and specific antibodies were detected in the NW. The dynamics of HI and NI antibody formation did not differ substantially, but there were individual differences in titres and persistence of antibodies. Convalescent sera always contained increased levels of HI and NI antibodies. In some cases, the titres of antibody to viral ribonucleoprotein did not increase.

Adolescent↗