Biomedical subjects

L V Beletskaia

Publications and source records attributed to L V Beletskaia.

94 records · Page 6Linked to original sources

PubMedDate not supplied

[An immunomorphological study of the effect of ionizing radiation on the nonlymphoid structures of the mouse thymus using monoclonal antibodies].

Expression of cytoskeleton components revealed with monoclonal antibodies was increased in epithelial cells of mouse thymus one day after total gamma-irradiation with doses of 0.5-4.0 Gy, while after the doses of 10 and 15 Gy it was decreased. The antigen expression was reversed to initial level 14 days after the dose of 4 Gy; thymocyte depletion was progressed and continuous massifs of epithelial cells was formed. The oval cysts and immunoglobulin deposits were appeared in thymus two months after irradiation with the dose of 4 Gy.

Animals↗

PubMedDate not supplied

[A method of detecting soluble immune complexes in tissue biopsies].

Pretreatment of cryostat slices of tissue biopsy specimens with 30-50% water ethanol mixture increases the rate of detection of immune complexes in the intercellular substance of the epidermis to 100% in patients with acantholytic pemphigus. This method permitted the detection of immune complexes in all the examined cases with benign familial Hailey-Hailey pemphigus, which has never been reported heretofore. The authors believe that 30-50% water-ethanol mixture is capable of soft denaturation of proteins, stabilizes the slightly affine antibody-antigen relations, and aggregates soluble immune complexes into larger protein formations, this preventing their washing out from tissues.

Antigen-Antibody Complex↗

PubMedDate not supplied

[Cardiomyocyte cytoskeleton in dilated cardiomyopathy and ischemic heart disease].

Summary. Vinculin, the protein of cardiomyocyte cytoskeleton in the myocardium of patients with a dilated cardiomyopathy and ischemic heart disease, was revealed by means of monoclonal antibodies and by a method of indirect immunofluorescence. Cytoskeleton of patients with dilated cardiomyopathy is disorganized, hypertrophic and contains greater amounts of vinculin than cytoskeleton of normal cardiomyocytes. This most likely negatively influences cell contractile capacity. The vinculin content was also higher in cardiomyocyte cytoskeleton of patients with ischemic heart disease but at a less degree than in patients with dilated cardiomyopathy.

Adolescent↗

PubMedDate not supplied

[Humoral immunopathologic changes in the myocardium after clinical allotransplantation of the heart].

Endomyocardial diagnostic biopsies, recipient heart removed at operation, endomyocardial biopsies of allotransplants and postmortem material were studied using immunofluorescence to specify immunopathological process and to detect humoral rejection. Altogether 306 samples from 55 patients were studied. In the early postoperative period (one year) 8 out of 18 patients with heart transplants repeatedly showed immunopathologic picture of acute humoral (vascular) rejection which was characterized by a widespread immunoglobulin G and complement fixation in the capillary walls accompanied by enhanced capillary permeability and fibrin deposition in intestitial tissue. Such patients often had graft dysfunction. 1 to 5 years after transplantation in 24 out of 37 patients discrete focal immunoglobulin and complement fixation was observed as one of chronic rejection component.

Adolescent↗