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Biomedical subjects

L Várady

Publications and source records attributed to L Várady.

10 recordsLinked to original sources
PubMed1994

[Extra-urogenital detection of Chlamydia trachomatis--perinatal transmission].

Infections produced by Chlamydia trachomatis reach an epidemic scale worldwide. Examination of 1,333 women with suspected cervicitis and other clinical symptoms revealed positive findings in 355 (27.5%). Of 72 examined men 25 (34.7%) were positive. Perinatal infection is confirmed also by positive evidence of Ch. t. in 28.9% neonates with conjunctivitis and 19% positive smears from the nasopharynx. The authors used the direct immunofluorescent method, Chlamyset of Orion Co., Finland.

Cervix Uteri↗
PubMed1992

[Detection of Chlamydia trachomatis in clinical material 1989-1990].

Examination of 276 smears and scrapings from the uterine cervix of women before delivery and during the puerperium revealed in 28.0% positive findings. Perinatal infection was confirmed by the presence of Chlamydia trachomatis in 35.5% neonates with conjunctivitis and 27.3% positive smears from the nasopharynx. The authors used the direct immunofluorescence method. For immunofluorescent staining they used Chlamyset of Orion Co., Finland.

Adolescent↗
PubMed1991

Antibody quantitation in seconds using affinity Perfusion Chromatography.

An extremely rapid assay technique for antibodies has been developed utilizing protein A or protein G bound to Perfusion Chromatography support matrices. Either dilute or concentrated samples are directly injected on a column that selectively binds antibody, which is quantitated directly by elution and UV absorbance. Due to the unique mass transport characteristics of the supports, total assay cycle times are typically 1 minute or less, with assays as short as 15 seconds possible. The assay system can accurately quantitate a 100,000:1 or greater dynamic range in sample concentration without sample dilution, is extremely repeatable and is easy to automate with conventional HPLC systems. Assay of antibodies in a wide range of sample types has been demonstrated.

Animals↗
PubMed1990

[Detection of Chlamydia trachomatis in clinical material 1985-1988].

Investigation of 529 smears from the uterine cervix of women before delivery, during the puerperium and before induced abortion revealed positive findings in 25.2% of women before delivery and in 19.3% of women before induced abortion. Perinatal infection was confirmed by a positive finding of Ch. t. in 12.8% neonates with conjunctivitis and 15.4% from the nasopharynx. The direct immunofluorescent method was used. In another group of women examined by the serological method ELISA, from a total of 182 sera in 41.7% the presence of IgG antibodies was detected. In cervical smears from 10 seropositive women in seven Ch. t. was positive.

Abortion, Induced↗
PubMed1988

Rapid high-performance affinity chromatography on micropellicular sorbents.

Short columns (30 x 4.6 mm I.D.), packed with 2-micron fluid-impervious silica microspheres with surface-bound Protein A or a lectin were used for fast separation and quantitation of immunoglobulins and glycoproteins by biospecific interaction chromatography. With stepwise elution, the total analysis time including column reequilibration did not exceed 3 min. In the assay of IgG with a stepwise change in pH best results were obtained with citrate buffer, which facilitated not only fast but also very sensitive analysis. The calibration curve was linear in the range 0.5-40 micrograms of human IgG. By using morpholinoethanesulfonic acid-4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid-acetic acid buffer with a linear decrease in pH from 6.0 to 4.0 and an increase in magnesium chloride concentration to 200 mM for elution, the subclasses of human IgG were separated at 40 degrees C above pH 4.0 in 3 min. Micropellicular concanavalin A and wheat germ agglutinin were used for rapid affinity chromatography of horseradish peroxidase and fetuin, respectively. The results suggest that micropellicular affinity sorbents afford fast and sensitive high-performance liquid chromatographic analysis by biospecific interaction chromatography. Although developed primarily for rapid analysis, the micropellicular Protein A exhibited unexpectedly high adsorption capacity (e.g., 4.5 mg human IgG per ml of wet bed volume). This suggests that such columns could be employed in preparative protein chromatography as well.

Chromatography, Affinity↗
PubMed1984

Molecular weight measurement of human satietin.

Attempts were made to estimate the actual molecular weight of satietin, an endogenous food intake inhibitor, by means of classical and high-performance gel filtration and gel electrophoresis. Satietin, which proved to be a glycoprotein, was isolated from human plasma. Sephacryl S-300 gel and TSK-GEL G 3000 SW columns were used as media for gel filtration and gel electrophoresis in the presence of sodium dodecyl sulphate (SDS-PAGE), respectively. A relatively good correlation was established between the molecular weight measurements carried out by different methods, although satietin as a carbohydrate-rich glycoprotein gave an underestimated value by gel filtration when the calibration graphs were constructed with simple proteins. The molecular weight of satietin was determined to be about 43,000 daltons by SDS-PAGE and 29,000 daltons by gel filtration when using a buffer containing 1% of sodium dodecyl sulphate.

Chromatography, Gel↗