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PubMed · 1931021

Antibody quantitation in seconds using affinity Perfusion Chromatography.

S P Fulton·M Meys·L Várady·R Jansen·N B Afeyan
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Abstract

An extremely rapid assay technique for antibodies has been developed utilizing protein A or protein G bound to Perfusion Chromatography support matrices. Either dilute or concentrated samples are directly injected on a column that selectively binds antibody, which is quantitated directly by elution and UV absorbance. Due to the unique mass transport characteristics of the supports, total assay cycle times are typically 1 minute or less, with assays as short as 15 seconds possible. The assay system can accurately quantitate a 100,000:1 or greater dynamic range in sample concentration without sample dilution, is extremely repeatable and is easy to automate with conventional HPLC systems. Assay of antibodies in a wide range of sample types has been demonstrated.

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AnimalsCalibrationChromatography, AffinityHumansImmunoglobulin GLigandsMiceNerve Tissue ProteinsReproducibility of ResultsSensitivity and SpecificityStaphylococcal Protein AJournal Article

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S P Fulton, M Meys, L Várady, R Jansen, N B Afeyan. 1991. Antibody quantitation in seconds using affinity Perfusion Chromatography.. https://pubmed.ncbi.nlm.nih.gov/1931021/

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