[Preparation of monoclonal antibodies against 185-SA of Streptococcus mutans].
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Animals↗
Biomedical subjects
L Xie
Publications and source records attributed to L Xie.
[An investigation on therapeutic results of extracapsular cataract extraction and intraocular lens implantation in diabetics].
44 cases (50 eyes) with diabetes and cataract were followed up for 6-30 months after extracapsular cataract extraction (ECCE) with intraocular lens implantation (IOL). No severe intra- and post-operative complications were found and 84% of eyes obtained post-operative corrected visual acuities > or = 0.5. The results suggest that the pre-operative hyperglycemia alone be not a contraindication of the operation. Pre-operatively, if the diabetes of patients with cataract can be controlled by diet or hypoglycemic agent, their glucose level in the blood remains normal and stable for a period of time and there are no other systemic and ocular complications, ECCE with IOL can be performed on such patients and the therapeutic results of the operation can be safe and reliable.
Adult↗
[A preliminary report of small incision cataract surgery by phacoemulsification].
The results of 34 cases with cataract extracted by phacoemulsification were preliminarily reported. Postoperatively, they achieved corrected visual acuities > 1.0 in 24 eyes (70.59%) in one week and in 25 eyes (73.53%) after 3 months. The post-operative astigmatism showed a tendency of change from with-the-rule shifting to against-the-rule. The rate of the corneal endothelial cell loss was 13.28%. No severe intra- and post-operative complications were found. It is concluded that the small incision, slight post-operative reaction and fast visual rehabilitation inherent in phacoemulsification procedures are more beneficial to patients than the conventional extracapsular cataract extraction.
Adult↗
[An experimental study on HSV-1 corneal latency by in situ nucleic acid hybridization].
An experimental study was performed on animal models for evaluation of the possibility of herpes simplex virus-1 (HSV-1) corneal latency by in situ nucleic acid hybridization. 20 normal New Zealand white (NZW) rabbits were used, and 3 x 10(6) PFU/ml of McKrae strain HSV-1 was inoculated bilaterally into the corneal stroma in 14 rabbit eyes. Of the 28 eyes, 22 developed typical herpes simplex keratitis (HSK). On the postoperative 60th day, 4 corneas with latent infection were transplanted into one unilateral eyes of each 4 non-infected NZW rabbits respectively and removed 2 weeks postoperatively. The corneal buttons were individually detected for the presence of HSV-1 antigen and nucleic acid sequences by using clonal IgG HSV-1 antibody and biotinylated HSV-1 DNA probe respectively. The results showed that the HSV-1 DNA sequences retained only within the corneal stromal layer with negative HSV-1 antigen staining. These results strongly suggest that the cornea be capable of harboring latent HSV-1.
Animals↗
[Advances in the pathological study of hepatitis C].
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Animals↗
[A clinical observation on intraocular lens implantation in high myopic eyes with cataract].
Extracapsular cataract extraction (ECCE) with intraocular lens (IOL) implantation was performed on 124 high myopic eyes with cataract of 101 cases whose mean period of follow-up was 16.4 months. Post-operatively, 95.97% of these eyes were relieved from blindness and 75.00%, from visual handicap. The incidence of posterior capsular opacity was 22.58% and only did 1.61% of the eyes occur retinal detachment. Intraoperatively, posterior capsular rupture occurred in 5.65% of the eyes. The results indicate that ECCE with IOL implantation performed on eyes with high myopia and cataract is effective.
Adolescent↗
A study on HSV-1 corneal potential infection by in situ nucleic acid hybridization.
PURPOSE: To evaluate the possibility of HSV-1 corneal latency by in situ nucleic acid hybridization in animal models. METHODS: 20 normal New Zealand White (NEW) rabbits were used, 14 of them were inoculated bilaterally with 3 x 10 PFU/ml of McKrae strain HSV-1 by intrastromal injection. 22/28 eyes developed typical herpes simplex keratitis (HSK) diseases. At 60 day postoperation (PI), 4 latent corneas were transplanted to one eye of 4 noninfected NZW rabbits and removed 2 weeks PI. Corneas at all time intervals of infection and two weeks after PKP were detected for presence of HSV-1 antigen and nucleic acid sequences by using clonal IgG HSV-1 antibody and biotinylated HSV-1 DNA probe individually. RESULTS: The results showed that the HSV-1 DNA sequences were retained within the corneal epithelium and anterior stromal keratocytes during acute diseases, while the corneas during latent infection and postoperation, the HSV-1 DNA sequences were retained only within the stromal layer with negative HSV-1 antigen staining. CONCLUSIONS: These results strongly suggest that the cornea may be capable of harboring latent HSV-1.
Animals↗
[A preliminary report on extracapsular cataract extraction with intraocular lens implantation in children].
Extracapsular cataract extraction (ECCE) with posterior chamber intraocular lens (IOL) implantation was performed on 94 eyes of children who were followed up for 6 approximately 30 months. IOLs were inserted in all of these eyes successfully with anterior vitrectomy used in several cases with traumatic cataract. The postoperative managements were similar to those in adults, but their complications were severer obviously than those of adults. Uveitis was the main postoperative complication which was characterized by severer anterior chamber reaction, longer duration and easier to form posterior iris synechiae and develop posterior capsular opacity. The follow-up results showed that the unilateral recovery rate from blindness is 90.1%, and 49.4% of these patients' corrected visual acuities were below 0.4. Because of the controversy on IOL implantation in children, it is the authors opinion that it should be cautious to perform IOL implantation on children and such an operation is not advocated for children under 3 years old. Children with unilateral cataract should be managed by experienced doctors, and it is very important to follow up carefully, to correct refractive error and carry out amblyopia treatment.
Adolescent↗
Applications of improved stoichiometric model in medium design and fed-batch cultivation of animal cells in bioreactor.
In our previous work (Xie and Wang, 1994a), a simplified stoichiometric model on energy metabolism for animal cell cultivation was developed. Fed-batch experiments were performed in T-flasks using this model in supplemental medium design (Xie and Wang, 1994b). In this work, the major pathways of glucose and glutamine metabolism were incorporated into the stoichiometric model. Fed-batch culture was conducted in a 2-liter bioreactor with appropriate process control strategies. Nutrient concentrations, especially glucose and glutamine, were maintained at constant but low levels through the automated feeding of a supplemental medium formulated using the improved stoichiometric model. The formation of toxic byproducts, such as ammonia and lactate (Hassell et al., 1991), was greatly reduced. The specific lactate production rate was decreased by 62-fold compared with batch culture in bioreactor and by 8-fold compared to fed-batch culture in T-flask using the previous stoichiometric model. Ammonia formation was also decreased compared with both the batch and fed-batch cultures. Most importantly, the monoclonal antibody concentration reached 900 mg l-1, an increase of 17- and 1.6-fold compared with the batch and fed-batch cultures respectively.
Amino Acids↗
124 cases of dyssomnia treated with acupuncture at sishencong points.
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Acupuncture Points↗
[An experimental study on cultivation of human trigeminal ganglionic cell in vitro and its sensitivity to infection of herpes simplex virus type 1].
We firstly report an experimental study on the sensitivity of primary cultural human trigeminal ganglionic cells infected by herpes simplex virus type 1 (HSV-1) in different time-points. The results indicate that the neuron of human trigeminal ganglia is the most sensitive cell type, the antigen of HSV-1 replicates rapidly within the cytoplasm and cell nucleus, the fibroblasts and glia cells are infected partly by the virus. Therefore, perhaps the neuron is the main harboring cell type.
Antigens, Viral↗
[The prognostic characteristics of hypertensive left ventricular hypertrophy in a rural population].
One hundred and twenty-six rural hypertensive patients with ECG left ventricular hypertrophy (HT-LVH) were followed up for 10 years to investigate the prognostic characteristics in a population with low prevalence of hypertension (2.93%) and low plasma cholesterol (4.59 +/- 1.00mmol/L). A cohort of age, sex, region and occupation matched hypertensives without LVH (HT, n = 163) and normotensives (NT, n = 275) served as controls. The major cause of death in rural hypertensives with ECG LVH in this study was stroke (56.8%). After stratification of BP, no significant difference of stroke risk was found between patients with LVH and hypertensives without LVH, while cardiovascular risk in the patients with LVH plus ST segment depression persisted. LVH per se seems not to be an independent factor for stroke. The high stroke rate in patients with LVH may be ascribed to the coexisting higher level of BP. After adjustment of coexisting hypertension, ECG LVH based on voltage only was not an independent risk factor of stroke and cardiovascular events, while LVH plus ST segment depression was closely related to cardiovascular events. In this low level, plasma cholesterol did not affect the mortality rate of stroke.
Adult↗
Comparison of secondary structures of insulin and proinsulin by FTIR.
Although the structure of insulin is known in great detail, that of proinsulin has been little investigated, except for a few CD and NMR studies. The secondary structures of human proinsulin are now compared with those of insulin by Fourier Transformed Infrared (FTIR) studies. The deconvolved and second derivative spectra of proinsulin and insulin in the amide I' band region are closely similar with peaks corresponding to alpha-helix, irregular helix, and 3(10) helix at nearly identical positions. For both proteins, the relative contents of the above structures as calculated from the peak areas are in good agreement with the values obtained from the known structure of crystalline porcine insulin. However, compared with insulin, proinsulin has markedly more unordered structures as indicated by the area under the peak at 1643.4 cm-1. In addition, both peak positions and relative areas for turn structure of the prohormone are different from those for insulin. It appears from the above that the A-and B-chain segments of proinsulin and insulin are similar in their secondary structures, especially in helices. The C-chain segment is largely unordered except in a few beta-turns.
Insulin↗
Hysteroscopic findings in women with IUD-induced menorrhagia.
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Adult↗
The prognostic characteristics of hypertensive left ventricular hypertrophy in a population with low prevalence of hypertension and low plasma cholesterol.
One hundred and twenty-six hypertensive patients with ECG left ventricular hypertrophy (HT-LVH) were followed for 10 years to investigate the prognostic characteristics in a population with low prevalence of hypertension (2.93%) and low plasma cholesterol (4.26 +/- 0.91 mmol/L). A cohort of age-, sex-, region- and occupation-matched, randomized hypertensives without ECG-LVH (n = 163) and normotensives (n = 275) served as controls. HT-LVH was found to be associated with an increased risk of overall death, stroke mortality and cardiovascular mortality. The major cause of death in rural hypertensives with ECG-LVH was stroke (56.8%). After adjusting BP and BP stratification, no significant difference of stroke risk was found between patients with and without LVH, while cardiovascular risk persisted in patients with LVH plus ST segment depression. LVH per se did not appear to be an independent factor for stroke death. Higher stroke mortality in patients with LVH may be ascribed to the coexisting higher level of BP. Furthermore, after adjusting for the coexisting hypertension, ECG-LVH in terms of voltage only was not related to the prognosis of stroke and cardiovascular death. The mortality rate of stroke was not affected by plasma cholesterol levels, as the levels seen were in a low range.
Adult↗
The pairing of the separated A and B chains of insulin and its derivatives, FTIR studies.
From the amide I bands of their deconvolved FTIR spectra, the S-thiomethyl derivatives of the insulin A, B, despentapeptide(26-30) B and desoctapeptide(23-30) B chains all contain significant amounts of ordered secondary structure. The intact B chain is considerably more ordered than either the A or the truncated B chains. Comparison of the spectra of the separated and mixed intact chains of insulin suggests further folding upon mixing of the chains leading to significant increases in ordered secondary structures, presumably because of stabilization by interaction of the chains. The interactions of the A chain with the DPI B chain appear to be weaker as compared to that with the intact B chain. The above results suggest that only the intact A and B chains contain sufficient structural information to recognize each other and interact to form a native-like structure which make the correct formation of the disulfide linkages possible.
Fourier Analysis↗
Histological distribution and developmental changes of tropomyosin isoforms in three chicken digestive organs.
Histological localization of tropomyosin isoforms in three digestive organs from embryonic and adult chickens was performed by using rabbit antisera against chicken skeletal muscle tropomyosin and against low-Mr-type tropomyosin from chicken small intestine mucosa. The former antiserum (named TM-SH) reacted with alpha, beta, and high-Mr-type isoforms, and the latter (named TM-HL) reacted with alpha, beta, high-Mr-type and low-Mr-type isoforms, alpha and beta Isoforms were detected in muscle cells of the muscular layer and the muscularis mucosa. Low-Mr-type isoforms, however, were detected along the cell membrane and cytoplasm of almost all nonmuscle cells, especially in terminal webs of epithelial cells. Developmental changes of tropomyosin isoforms in digestive organs were studied by two-dimensional gel electrophoresis and image analysis. The relative amounts of alpha and beta isoforms increased in the course of development, but those of low-Mr-type and high-Mr-type isoforms decreased.
Animals↗
Effect of dizocilpine maleate on monoamines and their metabolites in rat brain.
Systemic (ip) injection of dizocilpine maleate (DM, 0.1 and 0.5 mg.kg-1) increased the levels of 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid but did not bring about any noticeable change in the dopamine (DA) level in the striatum and limbic area. DM also increased the levels of norepinephrine in the limbic area and 5-hydroxyindoleacetic acid in the hippocampus. Amphetamine increased DA level and reduced DOPAC level in the striatum and limbic area. The behavioral manifestations revealed that DM predominantly evoked circling behavior and ataxia. The results indicate that the mechanism of the behavioral effect of DM may be different from that of amphetamine.
3,4-Dihydroxyphenylacetic Acid↗