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L Zimnoch

Publications and source records attributed to L Zimnoch.

At least 19 recordsLinked to original sources
PubMed2006

Activity of lysosomal exoglycosidases in human gliomas.

There is a lot of data suggesting that modifications of cell glycoconjugates may be important in progression of cancer. In the present work we studied activities of lysosomal exoglycosidases: beta-hexosaminidase and its isoenzymes A and B, beta-galactosidase and alpha-mannosidase, in human gliomas. Enzyme activity was determined spectrophotometrically based on the release of p-nitrophenol from p-nitrophenyl-derivative of appropriate sugars. The activities of the exoglycosidases tested were significantly higher in malignant glial tumors than in control tissue (normal brain tissue) and non-glial tumors. The highest activities of exoglycosidases were observed in high-grade gliomas, and a positive correlation of enzyme activities and degree of malignancy was noted. Our results suggest that lysosomal exoglycosidases may participate in the progression and dynamical development of glial tumors.

Brain Neoplasms↗
PubMed2004

Apoptosis phenomenon in the selected neoplasms of the glial origin.

Gliomas cause a therapeutic problem because of their localization and asymptomatic growth in the initial phase. Neoplastic growth is connected with disturbance between proliferation and apoptosis. In the study, we assessed the Bcl-2 family proteins involved in apoptosis in gliomas. The study comprised 61 patients with gliomas and based on tissue material sent for the diagnosis. Apoptosis was assessed in various types of gliomas and was defined by the apoptotic index (IA) and shown immunohistochemically with using Bcl-2, Bax and Bcl-x antibodies. The data were statistically analyzed. We found an increased percentage of the Bax (+) cells in less matured gliomas. A reverse dependence was revealed for Bcl-x. It was found that, probably in gliomas, the assessment of the Bcl-2 family proteins may serve only as an additional parameter for the evaluation of the disease course and the therapeutic success.

Apoptosis↗
PubMed2004

Study on carcinogenesis in chronic cholecystitis.

Not only bile but also chronic cholecystitis may play a role in gallbladder carcinogenesis. Numerous studies have revealed a close correlation between chronic inflammation and neoplasia. The experiments were conducted on paraffin sections, obtained from 377 surgically resected gallbladders with chronic cholecystitis. Immunohistochemical reaction was conducted on deparaffinized sections, using a monoclonal antibody against 8-hydroxydeoxyguanosine (8-OHdG), a biomarker of oxidative DNA damage. An increase was found in the expression of 8-hydroxydeoxyguanosine in chronic cholecystitis. The level of 8-OhdG expression is associated with inflammation intensity and disease duration. DNA damage, observed in chronic cholecystitis, indicates a correlation between chronic inflammation and gallbladder carcinogenesis.

Adolescent↗
PubMed2004

Proliferative activity of chosen central nervous system (CNS) neoplasms.

Gliomas are the most common neoplastic tumours of the central nervous system. The aim of the study was to evaluate the proliferative activity of chosen types of gliomas and to analyse their correlation with histological type, malignancy grade, location, size and clinical symptoms. The study involved patients with astrocytoma, anaplastic astrocytoma, glioblastoma, oligodendroglioma, anaplastic oligodendroglioma. The proliferative activity (the labelling index--LI) of glial cells was estimated, using immunohistochemistry. In studied groups, a positive correlation was noted between the proliferative activity and tumour size, but not between the proliferative activity and tumour location. The clinical symptoms were conditioned mainly by tumour location and, to a smaller extent, by its size.

Adolescent↗
PubMed2001

Localization of blood coagulation factors in situ in pancreatic carcinoma.

Blood coagulation is activated commonly in pancreatic carcinoma but the role of the tumor cell in this activation is undefined. Immunohistochemical procedures were applied to fixed sections of 22 cases of resected adenocarcinoma of the pancreas to determine the presence of components of coagulation and fibrinolysis pathways in situ. Tumor cell bodies stained for tissue factor: prothrombin: and factors VII, VIIIc, IX, X, XII, and subunit "a" of factor XIII. Fibrinogen existed throughout the tumor stroma, and tumor cells were surrounded by fibrin. Staining for tissue factor pathway inhibitor, and plasminogen activators was minimal and inconsistent. Plasminogen activator inhibitors -1, -2, and -3 were present in the tumor stroma, and on tumor cells and vascular endothelium. Extravascular coagulation activation exists associated with pancreatic carcinoma cells in situ that is apparently unopposed by naturally occurring inhibitors or the plasminogen activator-plasmin system. We postulate that such local coagulation activation may regulate growth of this malignancy. These findings provide a rationale for testing agents that modulate the blood coagulation/fibrinolytic system (that inhibit tumor growth in other settings) in pancreatic carcinoma.

Adenocarcinoma↗
PubMed2001

MIB-I as a proliferative activity marker of the multiform glioblastomas.

MIB-I is a proliferative activity marker of multiform glioblastomas which are the most frequent tumors of the central nervous system. They are characterizad by differential rate and prognosis. The aim of the study was to determine the proliferative activity of multiform glioblastomas and estimation of the correlation between tumors' proliferative activity and tumors' localization, size, patients' age and sex. 24 patients (18 females and 6 males) with multiform glioblastomas were analyzed. The mean patients' age was 52.1. The proliferative activity was calculated as a proliferation index: IP for MIB-I. Cells with positive reaction were determined by MIB-I which was compared to all neoplastic cells. The most frequent localization of the tumors were frontal and temporal lobes of the brain. The size of the tumors ranged from 2.5 to 5.3 cm (mean 3.9). Mean IP was 43.2 (SD+/-17.4). We found no correlation between IP MIB-I and localization of the tumor, patients' age and sex. There was a marginal statistically significant correlation between IP MIB-I and size of the tumor (p=0.005).

Antigens, Nuclear↗
PubMed2001

[Magnetic resonance proton spectroscopy in neurooncology--preliminary report].

UNLABELLED: MR proton spectroscopy (1H MRS) has been widely applied in characterisation and differentiation of brain tumors, staging, recurrence of pathologic process, post radiotherapy changes and other lesions mimicking neoplasm like abscesses. Despite of many studies performed over last 3 years in many countries spectroscopic pattern (phenotype) of brain tumors is still not well estimated and the role of each metabolite as an indicator of histopathologic grade and type of the tumor is still unknown. MATERIALS AND METHODS: In vivo 1H MR spectroscopy was prospectively performed in 36 patients with intracranial tumors (low grade gliomas, high grade gliomas, meningiomas and abscesses). Examinations was performed with 1.5 T system, using voxels of 8 cc. RESULTS: In high grade gliomas level of NAA was decreased, and consequently low ratios of NAA to other metabolites were obtained; lactate and choline peaks were markedly increased. In patients with meningiomas signal of NAA was reduced, while in abscesses group peaks of acetate and succinate were observed. Authors analysed ratios of metabolites in above-mentioned tumors. The method is especially useful in differential diagnosis in neoplastic and nonneoplastic cases. CONCLUSIONS: Our study confirms diagnostic value of 1H MRS in doubtful cases of intracranial tumors.

Brain Abscess↗
PubMed2000

Expression of prothrombin fragment 1+2 in cancer tissue as an indicator of local activation of blood coagulation.

Immunohistochemistry was applied to AMeX-fixed tissue sections of 12 adenocarcinomas of the stomach (seven intestinal adenocarcinomas and five diffuse carcinomas), 12 adenocarcinomas of the pancreas (nine ductal adenocarcinomas and three signet ring carcinomas), and 12 squamous cell carcinomas of the larynx obtained at surgical resection to examine the possibility of extravascular activation of blood coagulation in cancer tissues by exploring the in loco patterns of distribution of fibrinogen, a final product of blood coagulation, fibrin, and a by-product of coagulation reactions (prothrombin fragment 1+2). Gastric, pancreatic, and laryngeal cancers exhibited fibrinogen antigen in abundance throughout the tumor stroma. Fibrin was detected along the edges of nests of carcinoma cells and at the host-tumor interface. Prothrombin fragment 1+2 was present in the blood vessels in areas of neoangiogenesis at the host-tumor interface (gastric and pancreatic cancer tissues) and on the tumor cell bodies (pancreatic and laryngeal cancer tissues). The presence of prothrombin fragment 1+2 in cancer tissues appears to be a good indicator of coagulation activation and thrombin generation at the tumor burden.

Adenocarcinoma↗
PubMed2000

Morphometric and ultrastructural studies of the sciatic nerve regeneration in rats intoxicated with ethanol.

The aim of the study was to examine the process of sciatic nerve regeneration and changes in the dorsal root ganglia (from which sensory fibres of the sciatic nerve extend) in animals intoxicated with ethanol. The experiment used 20 rats, divided into two groups: control and treated. The treated animals were intragastrically given 2g/kg b.w. of ethanol in 25% aqueous solution. In both groups the right sciatic nerve was transected and then sutured. After 5 months the animals were anaesthetized. The left and the right spinal dorsal ganglia-L5 and sections from the non-operated and operated sciatic nerves were collected for analysis. Ultrastructural examinations and morphometric measurements were conducted. It was found that ethanol administrated to rats inhibited regeneration of the transected and then sutured sciatic nerve, impairing the growth of axons in the transected nerve and destroying the regenerating sensory ganglion cells. The mechanism of the changes described may be associated with axonal transport disorders or with the suppressed production of biologically active substances, which affect nerve regeneration.

Animals↗
PubMed1999

Expression of tissue factor and tissue factor pathway inhibitor in situ in laryngeal carcinoma.

The blood coagulation mechanism may support tumor progression by several mechanisms including promotion of cell proliferation and angiogenesis. Immunohistochemical procedures were applied to AMeX-fixed sections of twelve cases of squamous cell carcinoma of the larynx obtained at surgical resection to determine the presence and distribution of tissue factor (TF), tissue factor pathway inhibitor (TFPI), other coagulation factors, fibrinogen, and fibrin in situ. TF antigen was present in normal squamous epithelial cells and tumor cells, predominantly in immature tumor cells in the vicinity of the host-tumor interface. Tumor cells stained also for factors VII and X. Staining for TFPI antigen was demonstrated in the connective tissue stroma adjacent to the tumor, in microvascular endothelial cells, and in normal squamous epithelial cells. Fibrinogen and factor XIIIa were distributed throughout the tumor connective tissue stroma. Fibrin (thrombin-cleaved fibrinogen) was detected at the host-tumor interface and along the margins of tumor nodules. Tumor cells in carcinoma of the larynx express a functional, TF-initiated pathway of blood coagulation. Interpretation of these findings together with the results of clinical trials of inhibitors of TF-induced coagulation activation versus effects of inhibitors of TF expression suggest novel approaches to the experimental therapy of laryngeal carcinoma.

Blood Coagulation↗
PubMed1998

[Angiogenesis in brain neoplasms--biological and clinical aspects].

Solid tumors are dependent on angiogenesis for sustained growth, therefore anti-angiogenic treatment is a potential antineoplastic therapy. Glioblastoma multiforme has been considered a suitable candidate for such treatment. This review summarizes the fundamentals of angiogenic process in glioblastoma multiforme and in other types of brain tumors for which they represent a potential for anti-angiogenic therapy.

Animals↗
PubMed1998

[Local activation of blood coagulation in pancreatic tissue in chronic pancreatitis].

Thromboembolic events may complicate clinical course of chronic pancreatitis. It is accepted that trypsin plays a role in the pathogenesis of these abnormalities. However there is a lack of information about local activation of blood coagulation in pancreatic tissue in chronic inflammation and contribution of tissue factor (TF) to this process. Immunohistochemistry was applied to AMeX-fixed sections of tissues of ten cases of chronic pancreatitis to explore the presence and distribution of components of the coagulation system in situ. TF antigen was present in cells of ductules. Fibrinogen and fibrin were detected in the inflammatory infiltrates of the pancreatic tissue. The data suggest that there is local activation of blood coagulation in pancreatic tissue in chronic inflammation that depends on tissue factor.

Antibodies, Monoclonal↗
PubMed1997

Primary intracranial malignant fibrous histiocytoma--ultrastructural study.

Malignant Fibrous Histiocytoma is the most common soft tissue sarcoma in adults with the majority of cases that occur in patients between 50-70 years and 64% of cases occurs in male. Most commonly it arises in extremities (lower 49% and upper 19%), less often in the abdomen (16%) and very rare in head localization (3%) [5]. Typically macroscopically it is a multilobulated, gray-white, fleshy mass between 5-20 cm of diameter. In light microscopy this tumour has a highly variable morphologic pattern and manifests a broad range of histological appearance. It has been classified into the following subtypes: storiform-pleomorphic, myxoid, giant cell, inflammatory and angiomatoid. The first two subtypes are more frequent than others. The aim of this report is to present the electronmicroscopic features of giant cell type of MFH localized in head.

Adult↗
PubMed1997

Ultrastructural studies of the effect of pentoxyfylline on the hepatic cell of the rat with simultaneous administration of cyclophosphamide.

Ultrastructural examinations carried out on liver specimens collected from Wistar rats revealed that single administration of cyclophosphamide in a dose of 150 mg/kg body weight produced changes in the cytoplasmic structures, particularly in the smooth endoplasmic reticulum and mitochondria. The changes were reversible and subsided 14 days following cyclophosphamide administration. Administration of pentoxyfylline to rats in a dose of 30 mg/kg b.w./day for 10 days induced structural alterations in the intracytoplasmic structures of hepatocytes, similar to those caused by cyclophosphamide, although more intensified in the vicinity of the vascular pole. The pentoxyfylline-induced changes in hepatocytes retreated already 4 days after its withdrawal and can be considered adaptative. Simultaneous administration of both drugs to rats resulted in enhanced metabolic disturbances in hepatocytes and dissociation of adaptative mechanisms manifesting themselves in irreversible morphological changes in the intracytoplasmic structures and in the occurrence of collagen in the intercellular spaces.

Animals↗
PubMed1997

Morphometric and ultrastructural studies of the spinal sensory ganglia in the course of sciatic nerve regeneration in rats intoxicated with ethanol.

The aim of the study was to examine morphological changes in the spinal dorsal ganglia (from which sensory fibres of the sciatic nerve extend) in animals intoxicated with ethanol, in which the sciatic nerve was transected and then sutured. By means of morphometric methods using a computer system of picture analysis and in ultrastructural examinations it was found that ethanol administered to rats to drink for 3 months caused only slight morphological alterations in the sensory nerve cells of the spinal vertebral ganglia on the non-operated side. However, ethanol or its metabolites destroyed the regenerating sensory nerve cells of the spinal vertebral ganglia of the transected and then sutured sciatic nerve.

Alcoholic Intoxication↗
PubMed1996

[Cyclops type changes--a specific complication after anterior cruciate reconstruction--a clinical and histopathologic study].

Four cases of specific complication following 15 anterior cruciate ligament reconstructions using isometric vascularized patellar tendon tendon graft are presented. During "second-look" arthroscopy in all 4 cases a nodule in anterior knee compartment was found. The consistent microscopic finding within the nodule was the presence of disorganized dense fibroconnective tissue. Small bone debris were found in all cases. Arthroscopic debridement was performed resulting in proper joint function.

Adult↗