Biomedical subjects

Liang Lü

Publications and source records attributed to Liang Lü.

3 recordsLinked to original sources

PubMed2003

Rapid preparation of DNA microarray using PCR for hepatitis B and D virus detection.

OBJECTIVE: To prepare DNA microarray for detecting both hepatitis B and D virus as (HBV and HDV). METHODS: With the assistance of Oligo6.4 software, specific PCR primers targeting the conserved region of HBV and HDV were designed. The PCR products were purified and cloned into the pMD18-T vectors, followed by rapid identification. The recombinant plasmids were then extracted from positive clones and the target gene fragments underwent sequence analysis. RESULTS: The gene fragments of HBV and HDV were obtained by PCR, which were confirmed by sequence analysis to be specific gene fragments of HBV and HDV. CONCLUSION: Using PCR amplification products to prepare the DNA microarray is quick, simple and effective.

Hepatitis B virus↗

PubMed2003

[Preparation of gene chip probes for Bacillus anthracis protective antigen].

OBJECTIVE: To study the method for rapid preparation of the gene chip probes for Bacillus anthracis protective antigen (pag). METHODS: According to the sequences of pag published on PubMed, a pair of primers was designed to amplify the PA gene of Bacillus anthracis. Restriction enzyme digestion and AT clone were employed to rapidly screen out the recons of PA segments for the preparation of the gene chip probes. The DNA sequences of these segments were determined with 377 DNA Sequencer, and the resultant sequences analyzed with Bioinformatic software. RESULTS: With the designed primers, the pag 2,205 bp in length was amplified. After enzyme digestion and AT clone, 7 fragments of varied lengths were screened, which underwent analysis with the basic local alignment sequence tool (BLAST) and 377 DNA Sequencer. BLAST analysis showed that all the fragments cloned belonged to Bacillus anthracis. CONCLUSION: PCR in conjunction with restriction enzyme digestion and AT clone is rapid and simple to prepare the desired gene chip probes.

Antigens, Bacterial↗

PubMed2003

Quick preparations of human parvovirus B19 microarray probes using PCR.

OBJECTIVE: To prepare DNA microarray probes for the detection of human parvovirus B19. Method Specific PCR primers were designed with the Primer Premier 5.0 to amplify the conserved regions of human parvovirus B19 genome. The PCR products were cloned into the pMD-18 T vector. METHOD: Sequences analysis showed the PCR products conformed to the sequences contained in the genome of human parvovirus B19. RESULT: Sequences analysis showed the PCR products conformed to the sequences contained in the genome of human parvovirus B19. CONCLUSION: PCR amplification of the conserved and specific human parvovirus B19 genes is simple and effective to prepare the desired probes.

DNA Probes↗