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PubMed · 14625164

Quick preparations of human parvovirus B19 microarray probes using PCR.

Liang Lü·Wen-li Ma·Hong-min Wang·Xiao-dong Ma·Zhao-hui Sun·Wen-ling Zheng
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Abstract

OBJECTIVE: To prepare DNA microarray probes for the detection of human parvovirus B19. Method Specific PCR primers were designed with the Primer Premier 5.0 to amplify the conserved regions of human parvovirus B19 genome. The PCR products were cloned into the pMD-18 T vector. METHOD: Sequences analysis showed the PCR products conformed to the sequences contained in the genome of human parvovirus B19. RESULT: Sequences analysis showed the PCR products conformed to the sequences contained in the genome of human parvovirus B19. CONCLUSION: PCR amplification of the conserved and specific human parvovirus B19 genes is simple and effective to prepare the desired probes.

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DNA ProbesHumansOligonucleotide Array Sequence AnalysisParvovirus B19, HumanPolymerase Chain ReactionJournal Article

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Liang Lü, Wen-li Ma, Hong-min Wang, Xiao-dong Ma, Zhao-hui Sun, Wen-ling Zheng. 2003. Quick preparations of human parvovirus B19 microarray probes using PCR.. https://pubmed.ncbi.nlm.nih.gov/14625164/

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