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M Andrien

Publications and source records attributed to M Andrien.

44 records · Page 3Linked to original sources
PubMed1986

Factors involved in decreased detection of class II histocompatibility antigens on blood mononuclear cells from transplanted patients receiving immunosuppressive drugs.

Class II histocompatibility antigens were assessed by complement-dependent cytotoxicity on B enriched mononuclear cells and on purified monocytes isolated from the peripheral blood of renal patients before and after transplantation. After transplantation, a significant decrease of detection of DR alloantigens (cytotoxicity score before transplantation 3 +/- 0.1; after transplantation 0.95 +/- 0.1; p less than 0.001) was found. A similar defect was observed both on B enriched frozen cells and on purified monocytes isolated during the post-transplant period. Nevertheless, antigens could be adequately detected by a sensitive microabsorption method and by a procedure involving preincubation of mononuclear cells at 37 degrees C for 3 h followed by B cells enrichment by rosetting. The 37 degrees C induced reexpression could be inhibited by 10 micrograms/ml cycloheximide and by 50 microM monensin. No difference was found in the magnitude either of decreased expression or of temperature-induced reexpression between patients treated by CyS-Pred and those receiving Aza-Pred. It is concluded that transplanted recipients disclose a reversible defect of the expression of class II histocompatibility antigens probably related to perturbation of various steps of intracellular biosynthesis and traffic of these molecules.

Antibodies, Monoclonal↗
PubMed1986

HLA-B POT: a new HLA-antigen within the B7-cross reactive group.

In this report, a new HLA-B locus antigen is described (tentatively called POT). The antigen has been identified with antisera against the antigens that belong to the B7-cross reacting group. In a collaborative study, based on exchange of cells and sera, it was confirmed by population and family studies that the antigen is distinct from B7, Bw42 or Bw73 and is associated with Bw6. In absorption experiments with EBV-transformed cell lines, the POT-antigens removes preferentially anti-B7, Bw42 and Bw73 activity and to a lesser extent antibodies reactive with the B40 CREG-antigens.

Cross Reactions↗
PubMed1986

[Limits of conventional nutrition education: an example from a West African urban area].

The failure of conventional nutrition education, as practiced in Western Africa, is acknowledged by the author. This assessment follows the formative and summative evaluation of a nutrition education programme, directed towards the parents of hospitalized children, in a hospital, in the Ivory Coast. Often carried out within conventional health structures (maternal-child health centers, rural health centers, hospitals, etc...), the programme represents an addition to daily health care activities, with actions often too isolated and too limited to exert a decisive impact on public health. Most of the time, these programmes are conceived and implemented by health care workers who are inadequately motivated and trained in both nutrition and education. Poorly conceived messages that lack cultural, economic or social adaptation to the specified target population, authoritarian, unfeeling pedagogy, and inadequate educational tools lead to uncertain results. Results that are difficult to assess due to the absence of a clear definition of objectives and procedures for scientific evaluation. The author, in agreement with other recent studies, suggests that nutrition education should be viewed as the cumulative effect of all the various communication activities aimed at modifying those individual or community behaviours that affect the nutritional status of a given population. Multimedia nutrition education, however, can only be implemented once population needs and available communication channels have been identified. It is with the idea of promoting such multimedia activities that the network for nutrition education in Africa has been created (see p. 56).

Cote d'Ivoire↗
PubMed1985

[Induction of cell surface antigens on a lymphomatous cell line by gamma interferon].

The interferons are known to induce the expression of cell surface determinants. Gamma interferon, in particular, has been shown to enhance class II antigens (DR) on the cell surface. We used this gamma interferon to induce beta 2-microglobulin (beta 2 mu) and a minor, sex related transplantation antigen called H-Y on the surface of B lymphoma cells. The antitumor effect of interferon could thus be at least twofold: an antiviral effect already known and an increase of intercellular recognition (by cell surface marker enhancement); allowing the tumor cells to be "seen" better by cells of the immune system.

Antigens, Surface↗
PubMedDate not supplied

Analysis of the peripheral T-cell compartment in the MHC class II deficiency syndrome.

To analyse the impact of lack of MHC class II expression on the composition of the peripheral T-cell compartment in man, the expression characteristics of several membrane antigens were examined on peripheral blood lymphocytes (PBL) and cultured T cells derived from an MHC-class-II-deficient patient. No MHC class II expression could be detected on either PBL or activated T cells. Moreover, the expression of MHC class I was reduced both on PBL and in vitro activated T cells compared to the healthy control. However, the reduced expression of CD26 observed on the PBL of the patient was restored after in vitro expansion. Despite the presumably class-II-deficient thymic environment, a distinct but reduced single CD4+ T-cell population was observed in the PBL of the patient. After in vitro expansion, the percentage of CD4+ cells dropped even further, most likely due to a proliferative disadvantage, compared to the single CD8+ T-cell population. However, proliferation analysis showed that T-cell activation via the TcR/CD3 pathway is not affected by the MHC class II deficiency.

Antigens, CD↗