Biomedical subjects

M B Barnes

Publications and source records attributed to M B Barnes.

3 recordsLinked to original sources

PubMed2001

Bacterial biofilm formation under microgravity conditions.

Although biofilm formation is widely documented on Earth, it has not been demonstrated in the absence of gravity. To explore this possibility, Pseudomonas aeruginosa, suspended in sterile buffer, was flown in a commercial payload on space shuttle flight STS-95. During earth orbit, biofilm formation was induced by exposing the bacteria to sterile media through a 0.2-microm (pore size) polycarbonate membrane. Examination of these membranes by confocal microscopy revealed biofilms to be present and that these biofilms could persist in spite of vigorous agitation. These results represent the first report of biofilm formation under microgravity conditions.

Bacterial Adhesion↗

PubMed1988

Plasmid-linked maltose utilization in Lactobacillus ssp.

Five strains of Lactobacillus plantarum and 4 strains of Lactobacillus ssp. isolated from fresh meat contained between 1 and 5 plasmids ranging in Mr from 1.3 to 49 MDa. Plasmid-curing studies suggested that maltose utilization is associated with a 49 MDa plasmid (pML291) in Lactobacillus sp. DB29 and 34.5 MDa plasmids in Lactobacillus ssp. DB27, DB28 and DB31. Restriction digestion of pML291 and a putative plasmid deletion derivative, pML292, isolated from a maltose negative mutant of DB29, generated common restriction fragments. Southern blot DNA-DNA hybridization using pML 291 as a probe indicated that there is strong homology between putative maltose plasmids.

Blotting, Southern↗

PubMed1982

Sequence in which the amino acids on corn (Zea mays) become limiting for growing rats.

Three 21-day feeding experiments were conducted to determine the sequence in which the amino acids of corn become limiting for the growth of young rats. Diets consisted of corn fortified with minerals and vitamins, and supplemented with various combinations of essential amino acids. On the basis of improvements in growth rate and feed efficiency, the sequence of limiting amino acids in corn was determined to be: (1st) lysine and tryptophan--colimiting, (3rd) threonine, (4th) isoleucine, (5th) valine, (6th) methionine, and (7th) possibly histidine and phenylalanine--colimiting. Changes in blood urea levels generally confirmed this sequence. There was no limitation of either arginine or leucine, or of nonspecific nitrogen. Weight gains and feed efficiencies of rats fed a corn diet supplemented with the eight limiting amino acids were equivalent to published values for rats fed nutritionally adequate diets.

Amino Acids, Essential↗