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Plasmid-linked maltose utilization in Lactobacillus ssp.

M L Liu·J K Kondo·M B Barnes·D T Bartholomew
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Abstract

Five strains of Lactobacillus plantarum and 4 strains of Lactobacillus ssp. isolated from fresh meat contained between 1 and 5 plasmids ranging in Mr from 1.3 to 49 MDa. Plasmid-curing studies suggested that maltose utilization is associated with a 49 MDa plasmid (pML291) in Lactobacillus sp. DB29 and 34.5 MDa plasmids in Lactobacillus ssp. DB27, DB28 and DB31. Restriction digestion of pML291 and a putative plasmid deletion derivative, pML292, isolated from a maltose negative mutant of DB29, generated common restriction fragments. Southern blot DNA-DNA hybridization using pML 291 as a probe indicated that there is strong homology between putative maltose plasmids.

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Blotting, SouthernDNA, BacterialFood MicrobiologyLactobacillusMaltoseMeat ProductsNucleic Acid HybridizationPlasmidsJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.

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M L Liu, J K Kondo, M B Barnes, D T Bartholomew. 1988. Plasmid-linked maltose utilization in Lactobacillus ssp.. https://doi.org/10.1016/0300-9084(88)90207-6

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