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Biomedical subjects

M Ceruti

Publications and source records attributed to M Ceruti.

65 records · Page 4Linked to original sources
PubMedDate not supplied

Melphalan monitoring during hyperthermic perfusion of isolated limb for melanoma: pharmacokinetic study and 99mTc-albumin microcolloid technique.

BACKGROUND: The kinetics of melphalan leakage from extracorporeal fluid to the peripheral blood was studied in ten patients undergoing hyperthermic isolation perfusion of the lower limbs as an adjuvant treatment in high-risk melanoma. MATERIALS AND METHODS: Systemic leakage was monitored by a new technique using 99mTc-albumin microcolloid. Serial samples were drawn from a peripheral vein and from the perfusion circuit during surgical treatment and analysed by HPLC. RESULTS: The leakage measured with 99mTc-albumin microcolloid ranged from 1.5 to 18%/h (mean 8%/h). The average concentrations in the perfusate were 200-300-fold those found in the systemic circulation. A good correlation (R=0.945) was obtained between systemic AUC (0 to 1 hour) and leakage measured through the 99mTc procedure. Negligible toxicity was found and the survival rate yielded 92% of objective response. CONCLUSION: By studying the pharmacokinetic data of melphalan in the circuit and in the systemic circulation, we were able to validate the 99mTc procedure used during clinical perfusion. Moreover, considering the efficiency of the system as well as the minimum toxicity and the high survival rate, a reduction of perfusion time may be considered.

Aged↗
PubMedDate not supplied

[Maternal and foetal aminoacidaemia and amino acids contents of amniotic fluid in normal, gestosic and diabetic pregnancy (author's transl)].

The AA. have examined 9 cases of normal pregnancy, 5 cases of E.P.H. gestosis and 5 cases of diabetes mellitus, for which cases caesarean section had been planed, determining the aminogram of the amniotic fluid, of the mother's venous plasma and of the umbilical venous plasma; the 3 samples were performed about contemporaneously. Both in the normal pregnancies and in those complicated by E.P.H. gestosis and by diabetes mellitus, the concentration of amino acids was higher in the umbilical venous plasma than in that of the mother and higher in the latter than in the amniotic fluid, with the exception of glycine, taurine, glutammic acid and aspartic acid in the normal pregnancies, of taurine in the gestosic pregnancies, of taurine, citrulline, glutammic and aspartic acid in the diabetic pregnancies.

Amino Acids↗
PubMedDate not supplied

Metabolism of 7-(1,3-thiazolidin-2-ylmethyl)theophylline by rat liver microsomes. Evidence for a monooxygenase-dependent step in 1,3-thiazolidine ring cleavage.

Metabolic transformation of the mucoregulator and bronchodilator 7-(1,3-thiazolidin-2-ylmethyl)theophylline was studied in vitro with a rat liver microsomal preparation containing a NADPH-generating system. The only metabolite observed was 7-theophyllinacetaldehyde. In contrast to previous literature pointing out the chemical nature of 2-substituted thiazolidine ring cleavage, the formation of 7-theophyllinacetaldehyde was mediated by monooxygenase-dependent oxidation. Possibly an unstable sulfoxide was the first metabolic product, rapidly converted to 7-theophyllinacetaldehyde by hydrolysis. The sulfoxidation was apparently catalyzed mainly by flavin-containing monooxygenases, as selective thermal inactivation and methymazole significantly reduced the rate of formation of the metabolite. No N7-dealkylation pathway producing theophylline was detected, indicating a high regioselectivity in in vitro metabolism, due to the nucleophilicity of the sulfur atom.

Animals↗
PubMedDate not supplied

[Training individuals of the construction sector in the province of Bergamo: a shared path division and an operative proposal].

With this presentation it will be introduced the experience of the work group native of Bergamo who is taken care to plan the formation course for the professional figures of system in building. The group is formed from the greater agencies present on the territory, their work is to ensure the protection of the health and the emergency of the workers in the field of the constructions. The objective is to use the formation course in order to diffuse one culture of the emergency. In the specific one they will come to describe the various planning phases of the course of formation for employers that mean to elect himself Responsible of the Service of Prevention and Protection (RSPP).

Humans↗
PubMedDate not supplied

Metabolism of 7-(1,3-dithiolan-2-ylmethyl)-1,3-dimethylxanthine by rat liver microsomes. Diastereoselective metabolism of the 1,3-dithiolane ring.

The metabolic transformation of the antibronchospastic compound ABC-99 [7-(1,3-dithiolan-2-ylmethyl)-1,3-dimethylxanthine] was studied in vitro with a rat liver microsomal preparation containing an NADPH-generating system. Thirty percent of the ABC-99 was metabolized and the only metabolic pathway observed as the oxidation of the 1,3-dithiolane ring. Two distinct sulfoxides were formed diastereoselectively, the trans isomer being the major product in the ratio 7:3. In contrast to the 1,3-dioxolane ring of doxophylline, the 1,3-dithiolane ring of ABC-99 did not undergo oxidative opening through acetal carbon oxidation. Furthermore no N-dealkylation to theophylline was observed. This high regioselectivity in in vitro metabolism was most likely due to the nucleophilicity of the sulfur atom. The diastereoselective sulfoxidation was apparently catalyzed by flavin-dependent monooxygenases, as no effect was observed with CO treatment, whereas selective thermal inactivation significantly reduced the rate of sulfoxidation.

Animals↗
PubMedDate not supplied

Metabolism of 1,1-dichloro-cis-diphenylcyclopropane by rat liver microsomes.

The metabolic fate of the anti-estrogen 1,1-dichloro-cis-diphenylcyclopropane (Analog II), was studied in vitro with phenobarbital-induced rat liver microsomal fractions. The presence of five metabolites was directly or indirectly established. Biotransformation products were isolated by TLC and HPLC techniques and, when possible, the structures were confirmed through comparison with synthetic samples. The presence of an allyl chloride, highly reactive, metabolic intermediate was stated.

Animals↗
PubMedDate not supplied

[Labor in patients previously subjected to cesarean section: maternal and fetal aspects. Review of the literature].

In the present study the authors, on the basis of the existing literature, analyse the delivery problem in previous cesarean section patient, especially about maternal mortality and morbidity. After cesarean section vaginal delivery may occur only in selected patients, with precautionary measures and continuous monitoring in labor. The maternal mortality is lower in vaginal delivery patients after cesarean section than in iterative cesarean section patients; also the post operating complications are more frequent after iterative cesarean section. Perinatal mortality is in relation to uterine rupture, perinatal morbidity to iatrogenic prematurity and neonatal respiratory adaptation.

Cesarean Section↗
PubMedDate not supplied

[Natural antitumor compounds of vegetable origin].

This review deals with various aspects of biochemical cancer chemotherapy based on vegetable products. Nearly all isolated vegetable agents know to have in vitro or in vivo anticancer or immunostimulation activity are collected. These researches aim to develop selective antitumor agents.

Animals↗
PubMedDate not supplied

[Antiproliferative effect of an extract of Aspergillus terreus].

We have studied antiproliferative effect of Aspergillus terreus extract on various cell lines: I-407 normal human cells, L-929 normal murine cells, K-562 erythroleukemic human cells, IAD lymphoblastoid human cells and B 95-8 monkey lymphoblastoid cells. K-562 and lymphoblastoid cells showed higher sensitivity to the extract. Moreover, "a cytostatic dose" of the extract on K-562 cells (12.5 micrograms/ml showed a reduction of 3H-thymidine (but not of 14C-uracil) incorporation, leading us to hypothesize a prevalent action on DNA synthesis.

Animals↗