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M Cornet

Publications and source records attributed to M Cornet.

At least 73 records · Page 4Linked to original sources
PubMed1981

The location of San Angelo virus in developing ovaries of transovarially infected Aedes albopictus mosquitoes as revealed by fluorescent antibody technique.

Aedes albopictus adult female mosquitoes, transovarially infected with San Angelo (SA) virus, were examined by fluorescent antibody technique during various stages of ovarian development to determine how the virus enters the egg. Upon emergence from the day of adulthood, it was visible in the follicular epithelium, oocytes and nurse cells of the primary follicles. In the 72-hour period between the ingestion of blood and oviposition, there was a marked increase in the amount of viral antigen in the oocyte, indicating rapid virus accumulation. After oviposition, SA viral antigen was also seen in the secondary ovarian follicles. The observed sequence of infection of the mosquito ovariole with SA virus is analogous to that described with certain endosymbionts of insects.

Aedes↗
PubMed1978

[Isolation in east Senegal of a yellow fever virus strain from a pool of Aedes belonging to the subgenus Diceromyia].

During an epidemiological survey of yellow fever in Eastern Senegal, one strain of yellow fever virus was isolated in December 1976 from wild Mosquitoes. This first isolate obtained in nature from Aedes subgenus Diceromyia shows the primordial part these vectors may have in the area studied. It corroborates the existence of a selvatic focus of yellow fever in this region. It also gives information on the transmission cycle in a dry area.

Aedes↗
PubMed1977

Studies on intercellular adhesion. II. Promoting effect of serum and proteins on adhesion of neural retina cells to isotypic aggregates.

The quantitative assay described in the preceding paper (Pessac et al., '77) was used to study the effects of serum and various proteins on isotypic adhesion of chick embryo neural retina cells. Fetal bovine, chicken, horse, rabbit and human sera promoted cell adhesion to the same extent. The same sera also enhanced isotypic adhesion of cells from other organs showing that the cell adhesion promoting activity of sera was not organ specific. Neural retina (NR) cell collection in serum supplemented medium was not modified by protein synthesis or metabolic inhibitors and was temperature dependent with a maximum at 38 degrees C. The higher temperature does not seem to be required for repair of the cell surface after dissociation, but for the process of adhesion itself. Various serum fractions and egg albumin showed a cell adhesion promoting activity similar to that of sera.

Azides↗
PubMed1976

[Transovarian transmission of a Flavivirus, the Koutango virus, in Aedes aegypti L].

The Koutango virus Dak An D 5443 isolated from rodents of the genus Tatera belongs to the genus Flavivirus of the faily Togaviridae. This virus is very similar to the yellowfever, virus. It is experimentally transmitted from young Mouse to young Mouse by Aedes aegypti. The following observations show on one hand that this virus is found in the eggs of infected A. aegypti and on the other that the females from these eggs transmit the virus to the young Mouse by biting them.

Aedes↗
PubMedDate not supplied

Species-dependent differences in biotransformation pathways of 2-methylpropene (isobutene).

The biotransformation of 2-methylpropene, a gaseous alkene widely used in industry, was investigated in vitro in liver tissue of rats, mice, and humans. Interspecies comparison revealed that the lowest levels of the primary epoxide metabolite were detected in incubations of 2-methylpropene with human liver homogenate, followed by rat and mouse, respectively. Among the human liver samples, however, important interindividual variations were observed. Out of the 16 samples analyzed, only 2 contained measurable epoxide amounts, while in the other samples only traces were detectable. The involvement of rat liver cytochrome P450 2E1 in the activation of 2-methylpropene to its epoxide 2-methyl-1,2-epoxypropane has been established. The lower capacity of the mixed function oxidase system in human liver samples compared to rodents is confirmed. Concerning epoxide detoxifying enzymes, a high microsomal epoxide hydrolase activity was observed in human liver tissue and an intermediate in rat liver, while a low activity was measured in mouse liver. These findings were inversely correlated with the epoxide levels measured in vitro in liver tissue of the three species studied. It can be concluded that, as far as the in vitro metabolism of 2-methylpropene is concerned, neither mouse nor rat represents a good model for the human situation. Although, the same biotransformation pathways are involved, marked quantitative differences in epoxide levels were observed. The results indicate that human liver tissue is exposed in vitro to smaller concentrations of the primary metabolite 2-methyl-1,2-epoxypropane than rodent liver.

Adult↗
PubMedDate not supplied

[Technical note on the isolation of arboviruses by inoculation in young mice: preparation of the mosquito brei].

Studying the effects of centrifugation and deep freezing on the quantity of yellow fever virus in a grinded pool of mosquitoes, the authors followed the mortality rate of inoculated baby mice with twenty five artificially infected mosquitoes treated in four different ways. The statistical analysis of the results show that centrifugation and deep freezing have both an effect on the titer of virus and that the addition of the two treatments have an effect superior to the addition of the separate effects of each of them. The authors propose a new technic for the preparation of pools of mosquitoes, without centrifugation or deep freezing.

Animals↗