M E Hartley | PubMed Health

PubMed1995

The development of module evaluation: a Delphi approach.

This paper outlines an innovatory approach to curriculum evaluation in one of the six colleges of nursing that developed an English National Board (ENB) pilot scheme in nurse education (ENB 1987a). The method of evaluation is promoted as a means of facilitating a working partnership between student and teacher in which they can share in the dynamic process of systematic monitoring and reviewing of learning programmes to ensure changing needs are met. It is envisaged the strategy would interest planners or implementers of health care courses who seek a deeper insight into the perceptions and values their learners hold.

Curriculum↗

PubMed1987

A low-copy-number vector utilizing beta-galactosidase for the analysis of gene control elements.

A low-copy-number vector, pFZY1, with the multiple restriction site linker of M13mp18 inserted upstream from a promoterless beta-galactosidase (beta Gal)-coding lacZ gene has been constructed to provide a convenient and accurate system to analyze regulatory elements in vivo. The plasmid contains the oriF replication origin without the par locus and is present in the cell in one to two copies per genome. It is retained in the host by the presence of ampicillin, and each inserted promoter yielded consistent values of beta Gal activity under all the conditions tested. A series of tetracycline resistance (TcR) promoter fragments and lac promoter fragments have been compared in pFZY1 and the high-copy-number pKO-vector series. The transcriptional activity measured for different fragments containing the same TcR promoter varied within a six-fold range among the several constructs tested. Regulation of the wild-type lac promoter and mutants in pFZY1 was similar to that observed for lac promoters in the chromosome while their regulation in pKO-1mp18 was significantly affected by the high copy number, as expected.

Base Sequence↗