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M F Cisse

Publications and source records attributed to M F Cisse.

31 records · Page 2Linked to original sources
PubMed1991

[Neonatal bacterial infections: a retrospective study over 8 years].

In a retrospective study, one thousand and ten miscellaneous samples coming from infected newborns and tested at the bacteriology laboratory of A. Royer Children's Hospital from 1983 to 1991 were reviewed. These samples included 471 blood cultures, 114 pus of various origins, 410 cerebrospinal fluids and 15 urines. One bacterium or bacterial soluble antigens was detected in 294 samples (29.2%). Positivity percentage was 29.2% for septicemias, 68% for suppurations, 17.8% for meningitidis and 33.3% for urinary tract infections. There major species were identified: S. aureus (25.8%), Klebsiella spp (19.7%) and E. Coli (14.6%). Altogether, we isolated 156 enterobacteria (53%), 14 Gram negative bacilli (4.7%) and 124 cocci (42.1%) among which 19 streptococci (A, B, C) and 25 pneumococci. The most efficient antibiotics against all strains were AKN, CTX, CRO and GEN.

Anti-Bacterial Agents↗
PubMed1990

An epidemic of respiratory syncytial virus in elderly people: clinical and serological findings.

In 1984-1985, an outbreak of respiratory syncytial virus (RSV) infection occurred in two geriatric wards. Among 68 patients (mean age +/- SD = 82.5 +/- 12.5 with respiratory signs, 52 had signs caused by RSV infection. Among all patients, the clinical and serological attack rates were 61.2% and 75.0%, respectively. The most frequent clinical presentation was intensive coughing (96.1%) and fever (96.1%) associated with expectorate (63.5%). The duration of the respiratory symptoms was 5 to 7 days. The disease gradually resolved, although in eight (15.4%) patients complications occurred. For periods of up to 1 year after infection, 172 sera were obtained and tested by complement fixation test (CFT), fluorescent assays for titrating specific IgG, IgA, and IgM, and Western blotting. Specific IgM appeared in six (11.5%) of the infected patients and peaked 2 to 6 months after infection, and there was no significant correlation with severity of clinical symptoms. However, higher peak G and A antibody responses were observed in persons with rales (CFT: P = 0.008; IgG: P = 0.042; IgA: P = 0.020), cough (IgG: P = 0.034), sputum (IgG: P = 0.030), dyspnea (CFT: P = 0.024), conjunctivitis (CFT: P = 0.025), and bronchitis (CFT: P = 0.018). The temporal patterns of IgA and CFT results were found to be similar, whereas IgG peaked later, i.e., between 2 and 6 months. The patients with the most severe symptoms had the highest antibody titers obtained by conventional tests and by Western blots. Thus, RSV can be an epidemic pathogen among elderly persons, although this illness is usually mild.

Aged↗
PubMed1990

[Evaluation of ELISA using A60 antigen from Mycobacterium bovis BCG: specific IGG and IGM in mycobacterial infections].

The authors have evaluated an ELISA (A60-Tb, Anda biologicals) allowing the detection of specific IgG and IgM against A60 antigen from Mycobacterium bovis BCG during mycobacterial infections. This study included sera from 110 african subjects and from 71 French subjects distributed in 4 clinical groups: 55 tuberculous patients (I), 41 leprous patients (II), 33 pneumopathies (III) and 52 healthy subjects (IV). Serological results were compared taking as reference for the diagnosis of tuberculosis the positivity of culture and/or that of a direct examination, and for leprosy the positivity of a direct examination associated either with a Mitsuda's reaction or with an histopathological examination. IgG were found to be more discriminative than IgM. Considering together the results of groups I and II, the authors found a sensitivity of 95.8 p. cent and a specificity of 75.3 p. cent with threshold of 200 U/ml for specific IgG. Anti-A60 antigen antibodies obtained for groups I and II were significantly higher (IgG: p less than 0.0001; IgM: p less than 0.001) than those observed in other groups. African subjects presented IgG titers higher than those obtained by French subjects (p less than 0.0001). IgM response was more frequent among group II (97.6 p. cent) than group I (21.8 p. cent). However, IgG (26.9 p. cent) and IgM titers (30.8 p. cent) were detected among group IV. This test would allow a control of therapeutic efficacy with an additional interest for classifying borderline forms of leprosy.

Adult↗
PubMed1989

[Causes of infectious gastro-enteritis in children in Dakar].

Between February 1983 and May 1988, 1,157 stools from children aged under 15 years presenting with diarrhoea and admitted to a paediatric hospital in Dakar, Senegal, were examined for the presence of bacterial and parasitic agents. We looked for Campylobacter and rotavirus in only 245 and 111 samples respectively. Enterobacteria were detected most frequently (162/264; 61.3 per cent). Among these, we found 92 (34.8 per cent) strains of enteropathogenic E. coli; 40 strains (15.1 per cent) of Salmonella spp. and 30 strains (11.3 per cent) of Shigella spp. V. cholerae was present in only 2 faecal specimens (0,7 per cent). Eighty-one (30.6 per cent) parasitic agents were isolated, including a high proportion (13.6 per cent) of flagellae. Campylobacter jejuni and retroviruses were found in 2 (0.8 percent) and 15 (13.5 percent) stool respectively. The frequency with which these enteropathogenic agents were isolated remains in keeping with the global situation usually observed in Dakar for several years.

Adolescent↗
PubMed1989

[Electrophoretypes of the genome of rotaviruses isolated from Senegalese children].

Polyacrylamide gel electrophoresis allowing study of rotavirus genome was applied to 213 faecal specimens from children with acute gastroenteritis living in Dakar area, Senegal. We were able to define an electrophoretype in 41 cases (19.2%). Five different RNA electrophoretypes were shown. The electrophoretype 4 was the most frequent (41.4%). "Long" and "short" electrophoretic migration patterns were found in 36 and 5 cases, respectively. We found 34.1% of electrophoretypes in children aged from 6 to 11 months. The electrophoretypes 1, 3, 4 and 5 have co-circulated throughout the study period (March 1985 to August 1986). No mixed infections were found. Large surveys of this type would allow to evaluate the general situation in Senegal in urban areas as well as in rural areas.

Child, Preschool↗
PubMedDate not supplied

[Prevalence of Delta hepatitis in health workers in Dakar (Senegal)].

708 hospital workers in University Teaching Hospital of Dakar were tested to HBs antigen; among them 128 were positive and tested to Delta antibody, by ELISA Abbott kit. Seven (7) men and one woman were positive. However, the positivity of Delta antibody is neither linked to sex, nor to age of subjects. 87% of the Delta antibody carriers were found among the trainees, and 50% of them studied in the Odonto-Stomatology Institute.

Enzyme-Linked Immunosorbent Assay↗
PubMedDate not supplied

[Neonatal bacterial infections in the tropical zone].

One thousand and ten miscellaneous samples collected from infected newborns, were tested at the bacteriology laboratory of A. Royer Children's Hospital from 1983 to 1991. These samples included 471 blood cultures, 114 pus of various origins, 410 cerebrospinal fluids and 15 urines. One bacteria or bacterial soluble antigens were detected in 294 samples (29.2%). Positivity percentage was 29.2% for septicemia, 68.4% for suppurations, 17.8% for meningitidis and 33.3% for urinary tract infections. Altogether, we isolated 156 enterobacteria (53%), 14 Gram negative bacilli (4.7%) and 124 cocci (42.1%) among them 19 streptococci (A, B, C) and 25 pneumococci. Three major species were identified: S. aureus (25.8%), Klebsiella spp (19.7%) and Escherichia coli (14.6%). The most efficient antibiotics against all strains were AKN, CTX, CRO and GEN.

Antigens, Bacterial↗
PubMedDate not supplied

[Bacterial meningitis in a pediatric hospital in a tropical zone].

Purulent bacterial meningitides are a very important pediatric problem in tropical areas. From February 1983 to February 1988, 409 cerebrospinal fluid samples from children with purulent meningitides, aged under 15 years, admitted to the Pediatric Hospital Albert Royer, Dakar, were examined for the presence of bacterial agents. In 222 (54.2%) samples, one bacterial agent or soluble antigens were detected. Among these, 3 bacterial agents were most frequently identified: 94 (42.3%) Haemophilus influenzae, 71 (31.9%) Streptococcus pneumoniae and 25 (11.2%) Neisseria meningitidis. The increase in bacterial meningitides observed during cool and dry seasons was essentially due to Haemophilus influenzae and Streptococcus pneumoniae. The bacterial strains were found to be susceptible to antibiotics usually administered. However, almost 22% of Haemophilus influenzae strains were resistant to ampicillin or chloramphenicol. A high mortality (33%) was observed, especially with Streptococcus pneumoniae. The purulent bacterial meningitides are a most important pediatric problem in tropical areas.

Adolescent↗