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Biomedical subjects

M Fishman

Publications and source records attributed to M Fishman.

At least 91 records · Page 5Linked to original sources
PubMed1976

Studies on tolerance induction in vitro. II. Requirements for the generation and transfer of suppressor cell activity.

The induction of specific immunologic tolerance and its transfer has been studied in the in vitro response of unprimed rabbit spleen cells to solubilized phage antigens. Evidence presented shows that only viable cells transfer tolerance and that the responsible cells appear devoid of membrane-bound antigen but are sensitive to anti-thymocyte serum. It appears, therefore, that although the in vitro response to solubilized S-T2 has no demonstrable requirement for T "helper" cells, it is subject to the action of T "supp "suppressor" cells.

Animals↗
PubMed1975

In vitro studies on allotype suppression. II. Regulation of antibody synthesis by anti-allotype serum.

The regulatory effects of rabbit antibodies specific for light chain determinants (b locus) on the formation of rabbit serum immunoglobulins have been studied in an in vitro system which measures of the response of unprimed rabbit spleen cells to solubilized T2 phage antigen. Treatment of spleen cells from b4b4 rabbits with anti-b4 serum, which was either incorporated into the culture medium or employed in appropriate pulse treatment of the cells before culture, prevented the formation of T2 neutralizing antibodies by such cells. Spleen cells of heterozygous (b4b5) rabbits formed anti-T2 antibodies which could be shown to be divided between the b4 and b5 specificities. Incorporation of anti-b4 or anti-b5 serum into the culture medium suppressed the specific anti-T2 response and, except in the instances noted in the text, did not significantly change the level of T2 neutralizing antibodies marked with the alternate allelic determinant. These findings are discussed in the light of the compensatory formation of an alternate immunoglobulin type which occurs during allotype suppression in vivo.

Animals↗
PubMed1975

Studies on tolerance induction in vitro. I. Production of immunologically tolerant rabbit spleen cells and the transfer of tolerance to untreated cells.

Tolerance was induced in rabbit spleen cells by incubation with solubilized T2 phage (S-T2)2 at 37degrees C. Spleen cells thus treated maintained normal responsiveness to an unrelated antigen, S-SP82. Transfer of tolerance was demonstrated in in vitro in that the addition of washed tolerant cells caused suppression of the response of untreated cells to an immunogenic dose of S-T2. Evidence is presented that this suppression is not due to the transfer of tolerogenic quantities of antigen. Spleen cell populations depleted of adherent cells were still capable of being made tolerant and of transferring tolerance.

Animals↗
PubMed1975

In vitro studies on information transfer in cells from allotype-suppressed rabbits.

Data are presented which show that cells from allotypically suppressed rabbits resist in vitro "rescue" attempts in which informational ribonucleic acid (i-RNA) coding for the suppressed allotypic marker is used. It is shown that peritoneal exudate cells from such thoroughly suppressed animals contain cells that yield i-RNA coding for the suppressed marker, and that central lymphatic tissue possesses cells that produce Ig of the suppressed type.

Animals↗