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Biomedical subjects

M Fu

Publications and source records attributed to M Fu.

At least 127 records · Page 7Linked to original sources
PubMed1998

[Study on the adhesion of an endemic strain of Streptococcus mutans serotype C to acquired pellicle. II. Isolation and extraction of adhesins of an endemic strain of Streptococcus mutans serotype C].

Surface proteins of S. mutans WD9463 A(c) were separated by DEAE-Sephadex A25 Chromatography and Sepharose C1-6B Chromatography. The adhesins were distinguished by bacterial adhesin inhibition experiment and were identified by PAGE, SDS-PAGE, IEP-PAGE, immunol diffusion test, GTF activity test and annalization of the sorts of amino acid. The results showed P1, two proteins with molecular weight of 117 kD and 127 kD and two proteins with GTF activity could inhibit the adhesion effectively. On the other hand, another kind of GTF could improve the adhesion of the strain. These indicated that S. mutans may be many kinds of adhesins.

Adhesins, Bacterial↗
PubMed1998

Primary left atrial malignant mesenchymoma: a case report.

Primary malignant mesenchymoma involving the heart is extremely rare. It usually has a poor prognosis. We report a case of malignant mesenchymoma originating in the left atrium with protrusion into the right pulmonary veins. Clinically, this patient presented with congestive heart failure, hemoptysis and systemic arterial embolization. Transthoracic and transesophageal echocardiography revealed a huge mass originating in the posterior wall of the left atrium with extension into both upper and lower right pulmonary veins. An elevated pulmonary arterial wedge pressure, moderate pulmonary hypertension and stenosis of the lower abdominal aorta were found during cardiac catheterization. Magnetic resonance imaging with angiography disclosed a tumor mass over the abdominal aorta above the bifurcation with protrusion into the right common iliac artery. This patient underwent surgical resection of the cardiac tumor and postoperative adjuvant chemotherapy. The pathological finding of a tumor containing several cellular types of sarcoma confirmed the diagnosis of malignant mesenchymoma.

Adult↗
PubMed1997

Endotoxin-induced macrophage gene expression depends on platelet-activating factor.

BACKGROUND: The development of multiple organ failure in septic patients is due to a systemic inflammation orchestrated by macrophages (Mphi). Elucidation and control of the mechanism involved in Mphi activation in sepsis is crucial to improving survival. An early event of Mphi activation involves the hydrolysis of membrane phospholipid by phospholipase A2 (PLA2) and subsequent generation of platelet-activating factor (PAF). OBJECTIVE: We designed this study to test the hypothesis that Mphi gene expression depends on PAF. DESIGN: Rabbit alveolar Mphi were obtained by bronchoalveolar lavage and were stimulated with 10 ng/mL of Escherichia coli endotoxin lipopolysaccharide (LPS), PAF (1 micromol/L), LPS+/-CV3988 (10 micromol/L), a PAF receptor antagonist, or LPS+/-PLA2 inhibitors: AACOCF3 (50 micromol/L) or manoalide (10 micromol/L). After 4 hours of incubation, Mphi tumor necrosis factor (TNF) messenger RNA (mRNA) expression was assessed by Northern blot analyses. The TNF production in the Mphi supernatant was measured by L929 bioassays. RESULTS: The LPS-stimulated Mphi expressed increased levels of TNF mRNA and produced an enormous amount of TNF. CV3988, a PAF antagonist, inhibited LPS-induced TNF mRNA. Furthermore, inhibiting PAF production with AACOCF3, or manoalide, also inhibited LPS-induced Mphi TNF mRNA expression. The effect of PAF depends on changes in intracellular calcium concentration. Inhibitors of calcium flux attenuated the PAF effects on LPS-stimulated Mphi. CONCLUSIONS: Our data suggest that LPS-induced Mphi gene expression is mediated by PAF. It is likely that modulation of PAF production or activity may be beneficial in down-regulating the overactivity of Mphi in sepsis.

Animals↗
PubMed1997

Transradial coronary angiography and angioplasty in Chinese patients.

Radial artery punctures for diagnostic coronary angiography or coronary balloon angioplasty were performed in 211 patients with a success rate of 98% (207 patients). In the four failed transradial accesses, the procedure was accomplished via the transfemoral route. Major local vascular complications included one arteriovenous fistula, one pseudo-aneurysm, and one ischemic contracture of the right hand. Reduced radial pulses were noted in 25 (12%) patients at follow-up without ischemic manifestations. Transradial diagnostic coronary angiography was successfully completed in 184 (98%) of 187 patients. The technical success for balloon angioplasty was obtained in 73 (97%) of 75 patients. Clinical success was observed in 68 (91%) patients; balloon angioplasty resulted in one nonfatal myocardial infarction and four late deaths (3 cardiac and 1 stroke). We believe that transradial catheterization for diagnostic coronary angiography and balloon angioplasty in our relatively small built Chinese population is a safe and practical alternative approach.

Adult↗
PubMed1997

Macrophage TNF mRNA expression is modulated by protease inhibitors.

BACKGROUND: Nuclear factor kappa B (NF kappa B) is an important transcriptional activator protein and is a crucial component of the host's response to infection. The activation of NF kappa B is correlated with the phosphorylation of inhibitory kappa B (I kappa B) and its subsequent degradation. We hypothesized that protease inhibitors which prevented I kappa B degradation could inhibit the macrophage gene activation and reduce the production of inflammatory cytokines. METHODS: Rabbit alveolar macrophages (M phi) were obtained by bronchoalveolar lavage. M phi were exposed to Escherichia coli lipopolysaccharide (LPS) (10 ng/ml) in the presence of various concentrations of protease inhibitors, either N-tosyl-L-phenylalanine chloromethyl ketone (TPCK) or N-benzoyl-L-tyrosine ethyl ester (BTEE). Total RNA was extracted for Northern blot assay of tumor necrosis factor (TNF) mRNA expression using a rabbit genomic DNA probe. Total nuclear extracts were also obtained for the measurement of the NF kappa B activity with the electrophoretic mobility shift assay. The TNF production in the M phi supernatant was measured by L929 bio-assays. RESULTS: NF kappa B activity induced by LPS was inhibited by either BTEE or TPCK. Inhibition of NF kappa B activity by these agents also prevented TNF mRNA expression and TNF production induced by LPS. The cellular mechanism leading to NF kappa B activation was further studied. TNF mRNA expression and NF kappa B activation were inhibited by D609, a phospholipase C (PLC) inhibitor, as well as by protein kinase C (PKC) inhibitors. In addition, direct stimulation of PKC led to NF kappa B activation and TNF mRNA expression. CONCLUSIONS: These data suggest that TNF mRNA expression of LPS-stimulated M phi is mediated through NF kappa B, NF kappa B activation is intimately regulated by the PLC signaling pathway.

Animals↗
PubMed1997

The discovery and a study of the adventitia rectalis, a fibrous layer of the rectal wall.

In sacral anoplasty, it is frequently found that an outer layer of the rectal wall, which resembles the structure of the taenia bands of the colon, limits the elasticity of the rectum making it different from the small intestine, hence preventing the rectal stump from being advanced to reach the perineum. Results of autopsy studies on 40 human babies and 40 dogs showed that a special collagenous fibrous layer called adventitia rectalis was found to limit the free expansion of the rectal wall. This was responsible for keeping the rectum a straight tubular shape with a fixed volume. This tissue plays an important role in bowel movement by defining a threshold of intrarectal pressure in the bowel reflex of defecation and by transmitting the expulsive force from the abdomen above down to the anal opening. This discovery may explain why (1) in Hirschsprung's disease, the preservation of the rectal wall in Soave's or Rehbein's procedure or partial preservation in Duhamel's operation will preserve better bowel function; (2) in atresia ani, rectal tapering results in better bowel control than rectal pouch amputation; and (3) in sacral anoplasty, multiple releasing incisions of adventitia rectalis at different levels will lengthen the rectal stump to about double its original length to reach the perineal incision, and the divided fibers will soon regenerate.

Anal Canal↗
PubMed1997

Parathyroid Ca(2+)-conducting currents are modulated by muscarinic receptor agonists and antagonists.

Parathyroid cells express Ca(2+)-conducting cation currents, which are activated by raising the extracellular Ca2+ concentration ([Ca2+]o) and blocked by dihydropyridines. We found that acetylcholine (ACh) inhibited these currents in a reversible, dose-dependent manner (50% inhibitory concentration approximately equal to 10(-8) M). The inhibitory effects could be mimicked by the agonist (+)-muscarine. The effects of ACh were blunted by the antagonist atropine and reversed by removing ATP from the pipette solution (+)-Muscarine enhanced the adenosine 3',5'-cyclic monophosphate (cAMP) production by 30% but had no effect on inositol phosphate accumulation in parathyroid cells. Oligonucleotide primers, based on sequences of known muscarinic receptors (M1-M5), were used in reverse transcriptase-polymerase chain reaction (RT-PCR) to amplify receptor cDNA from parathyroid poly (A)+ RNA. RT-PCR products displayed > 90% nucleotide sequence identity to human M2- and M4-receptor cDNAs. Expression of M2-receptor protein was further confirmed by immunoblotting and immunocytochemistry. Thus parathyroid cells express muscarinic receptors of M2 and possibly M4 subtypes. These receptors may couple to dihydropyridine-sensitive, cation-selective currents through the activation of adenylate cyclase and ATP-dependent pathways in these cells.

Acetylcholine↗
PubMed1997

[Finite element analysis of force produced by "T" loop retraction archwire].

This study introduced three presumed springs to imitate the three dimensional bolstering function of the bracket-tooth-periodontal tissue on the archwire with three dimensional finite element method (FEM), and, systematically analysed the force system produced by the "T" loop retraction archwire on the incisors. The following conclusions were drawn; (1) The activation of the "T" loop retraction archwire will produce extrusion and root lingual torque force as well as horizontal force on the incisors; (2) Adequate torqul on the incisor segment and gable bend mesial to the T-loop are necessary to control the position of the anterior teeth while they are being retracted; (3) retraction archwire should not be activated too much; (4) Lighter wire will produce milder and more durable forces.

Biomechanical Phenomena↗
PubMed1997

[The experimental study about the relations between Chinese herb-epimedium leptorrhizum stearn (CH-ELS) and endogenous cAMP in alveolar bone of orthodontic tooth in rats].

The aim of this study was, to explore the mechanism of CH-ELS promoting bone remodelling of orthodontic tooth in rats. 11 male sprague-Dawleg rats was divided into 3 groups. Group I (normal control group) Group II (applied force, no injection) Group III (applied force, injection). The experimental period was 3 days. The radioimmunoassay (RIA) method were used to investigate the effects of CH-ELS on the content of endogenous cAMP in alveolar bone of orthodontic tooth in rats. Results indicate that CH-ELS has no significant effects on endogenous cAMP in alveolar bone of orthodontic tooth, perhaps, cAMP were not involved to regulate the mechanism of CH-ELS promoting bone remodelling. To explore the mechanism of bone remodelling of orthodontic tooth is more significant in orthodontic clinic.

Alveolar Process↗
PubMed1997

[DNA polymorphisms of apolipoprotein A I gene in Chinese endogenous hypertriglyceridemics].

The restriction fragment length polymorphisms (RFLPs) of apolipoprotein(apo) A I gene were studied using polymerase chain reaction (PCR) in 69 endogenous hypertriglyceridemics (HTG) and 74 healthy subjects from a population of Chinese Han nationality in Chengdu area. The loci studied included Msp 1 within intron 3 of the apo A I gene; Xmn I, 5' to the apo A I gene; and Pst I, 3' to the apo A I gene. The results showed that both in HTG group and control group M1, X1 and P1 alleles were the major alleles and homozyous M1M1, X1X1 and P1P1 genotypes were the most frequent ones. The frequencies of rare M2 and X2 alleles in Chinese were significantly higher than those in European Caucasians (0.293 vs 0.111, P < 0.0006, 0.286 vs 0.130, P < 0.004), but no differences were found in the frequency of rare P2 allele (0.071 vs 0.046, P > 0.05). The frequency of rare M2 tended to increase in the HTG group when compared with the control group (0.353 vs 0.275, P > 0.05). Both in the HTG group and control group, subjects with genotype M2M2 had a higher serum mean concentration of TC, apo C II and higher serum TG/HDL-C ratio compared with the subjects with the genotypes M1M1 and M1M2 (P = 0.05, P < 0.03, P < 0.04), and the serum TG, apo C II and apo E levels had a tendency to increase. The subjects with the genotypes P1P2 and P2P2 had a higher serum apo A II levels compared with the subjects with the genotype P1P1 (P < 0.04). The data show that the only Msp I RFLP within the intron 3 of apo A I gene is associated with endogenous hypertriglyceridemia to some extent in Chinese population.

Adult↗
PubMed1997

[Oxidative modification of serum LDL, VLDL and HDL induced by fed on high cholesterol diet in vivo in rabbits].

Many lines of evidence suggest that LDL is oxidized in vivo and that ox-LDL is present in the artery wall. But the oxidation of VLDL and HDL in vivo has not been reported yet. A study on effects of high cholesterol diet on oxidative modification of LDL, VLDL and HDL in rabbits was made. The control group (n = 8) was fed on routine diet, and the experimental group (n = 8) on high cholesterol diet (routine diet supplemented with 5% lard, and 0.5 g cholesterol a day for each rabbit) for 12 weeks. The serum LDL, VLDL and HDL were isolated by the density gradient ultra centrifugation. The oxidative modification of LDL, VLDL and HDL was identified by agarose gel electrophoresis, absorbance at 234 nm and fluorescence of TBARS. The results showed that serum TC, TG and TBARS in the experimental group were significantly higher than these of the control group (P < 0.01). The electrophoretic mobility of LDL, VLDL and HDL was increased, and absorbance at 234 nm and TBARS of LDL, VLDL and HDL in the experimental group were significantly higher than these of the control group (P < 0.01). These results suggest that not only LDL but also VLDL and HDL were oxidatively modified in vivo in rabbits after fed on high cholesterol diet.

Animals↗
PubMed1997

[The serum lipid and apolipoprotein levels of middle-aged male hyperlipidemics in Chengdu district].

It has been evidenced that cardiovascular diseases (CVD) relate with many risk factors and serum lipids play and essential role in the development of CVD. In order to further study hyperlipidemia (HL) in the middle-aged males, we analysed the body mass index (BMI), fast blood sugar (FBS), serum lipids and apolipoproteins A I, A II, B100, C II, C III and E in 223 male HL patients aged 41-60 and 349 normal male subjects who matched the HL patients in age. The result showed that the increase of serum triglycerids (TG), was as might be expected, the major characteristic of the middle-aged male HL patients in Chengdu District. Besides the serum lipid and apolipoprotein B100, C II, C III and E levels, the BMI and FBS levels in the IIb, IV and V types of HL patients were significantly higher than those in the normal subjects. It also showed that in TG increased patients the increased percentages of serum lipids were significantly higher than those of apolipoproteins. The age distribution in HL patients revealed that only 25% of the HL patients were between 41 to 50 years old, while 50% in the 56-60 year-old group. The relationship between apolipoprotein levels and serum lipid metabolism is also discussed. The results suggest that the 51-60 year-old males should pay attention to diet and increase physical activities to reduce incidence of HL which is directly associated with CVD.

Adult↗
PubMed1997

[Optimization of technical parameters for processing radix Aconiti coreani].

Based on the determination of guanfu A, hypaconitine, and total alkaloids, along with the experiment of acute toxicity of sliced Radix Aconiti Coreani and in compliance with the quality standard stipulated in pharmacopeia-surface features cross section colour and odor of sliced Radix Aconiti Co-reani the technology of processing Radix Aconiti Coreani has been optimized to be steaming the drug for four hours.

Aconitine↗
PubMed1997

[Orthodontic treatment in unilateral cleft lip and palate patients with crossbite].

Serious malocclusion is often seen in the cleft lip and palate patients. Orthodontic treatment is very complex and difficult. The aim of this study was to investigate the characteristics of the treatment in this kind of the patients through the clinical treatment of the thirty-two patients. We conclude (1) Many patients need alveolar bone grafting in order to award good arch form and occlusion. (2) It need longer time in expanding of the upper arch and retaining. (3) Main changes after treatment of edgewise appliance took place in dental-alveolar region. (4) 34.3% of the patients need orthognathic surgery to improve their profile. So it indicated that reverse headgear should be used in the early time for the cleft patients to get a good treatment result.

Adolescent↗