[Prenatal diagnosis in the Genetic Counseling Department of the Child Health Center].
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Chromosome Aberrations↗
Biomedical subjects
M Grabowska
Publications and source records attributed to M Grabowska.
The involvement of serotonin in the mechanism of central action of apomorphine.
The paper reviews and summarizes the results obtained in a series of experimental papers on the action of apomorphine on central serotonin mechanisms. Apomorphine accelerates utilization of serotonin in the central nervous system, this effect being a consequence of stimulation of central dopamine receptors. The activation of serotonergic mechanisms may be responsible for the hypothermizing action of apomorphine in rats and mice, pi modulates the locomotor stimulation produced by apomorphine in the rat, and does not influence the apomorphine-induced stereotypy in the rat.
Animals↗
Effect of butobendin on dopamine beta-hydroxylase in vitro.
It has been found that dopamine beta-hydroxylase (DBH) (E.C.1.14.17.1), a key enzyme in the multistep process of adrenaline formation, isolated from blood serum by fractionated salting out procedure, is an allosteric enzyme with a sigmoidal kinetics and positive cooperativity in binding of the substrate and effectors. Butobendin, a double ester of 2-aminobutanol and trimethoxybenzoic acid, a new interesting compound showing various pharmacological and metabolic properties, inhibits DBH activity stereospecifically, only in the configuration 2S,2'S, decreasing DBH Vmax. This noncompetitive inhibitory effect of butobendin was stronger than the feed-back DBH inhibition by adrenaline and noradrenaline or by quinidine used as a standard antiarrhythmic compound. At high butobendin concentration (1.55 microM) the DBH activity was stabilized at about 50% of initial activity. This indicates that tetrameric enzyme binds substrate to half-of-its-binding-sites. At low substrate (tyramine) concentration (1-5 mM), a sigmoidal kinetics was preserved only at low butobendin concentration (0.155-0.31 microM). At higher butobendin concentrations (0.77 microM) a linear kinetics of DBH appeared and Km value decreased, indicating an increase in affinity of enzyme to the substrate. Interesting regulatory properties of DBH and butobendin action deserve further attention, especially for understanding a therapeutic action of butobendin in some types of heart rhythm disturbances.
Anti-Arrhythmia Agents↗
[Detection of defects in components of antigen I and its significance in transfusiology].
The authors present the results of family investigations carried out in a healthy man whose serum contained anti-IDBH alloantibodies. In the erythrocytes of the propositus and one of his brothers defects in ID and IS components were found. At the same time increased expression of IT component was observed in both these men and in their brother. In the erythrocytes of the remaining family members no abnormalities in the components of I complex was found. The authors stress the importance of detection of these defects in the practice of transfusiology in patients with autoimmunohaemolytic anaemia.
ABO Blood-Group System↗