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M HEIDELBERGER

Publications and source records attributed to M HEIDELBERGER.

At least 19 recordsLinked to original sources
PubMed1964

CROSS-REACTIONS OF PNEUMOCOCCAL TYPES. QUANTITATIVE STUDIES WITH THE CAPSULAR POLYSACCHARIDES.

Data are given on the amounts of antibody nitrogen precipitated in the crossreactions, often reciprocal, of the specific capsular polysaccharides of the pneumococcal type pairs II and XX, II and XIX, VII and XIV, VII and XVIII, VII and XIX, VIII and XVIII, VIII and XIX, X and XIV, and X and XX. Since little is known of the fine structures of the polysaccharides of Types VII, X, XIX, and XX, rigorous correlations between chemical structure and specificity can not be made, but several tentative conclusions are drawn.

Agglutination↗
PubMed1962

Immunochemistry of the pneumococcal types II, V, and VI. III. Tests with derivatives of the specific polysaccharides of types II and VI.

Rebers, P. A. (Rutgers, The State University, New Brunswick, N. J.), E. Hurwitz, M. Heidelberger, and S. Estrada-Parra. Immunochemistry of the pneumococcal types II, V, and VI. III. Tests with derivatives of the specific polysaccharides of types II and VI. J. Bacteriol. 83:335-342. 1962.-The type-specific pneumococcal polysaccharides, S II and S VI, were oxidized with periodate, and the capacity of the derivatives for precipitation by antibodies and adsorption by erythrocytes was studied. The derivatives were further modified by reduction, oxidation, and condensation with urea. Oxidation of S VI with periodate reduced its precipitation of homologous antibodies much less than did similar treatment of S II. About one-half of the components are attacked in both cases, but in S II, glucuronic acid end groups, the chief determinants of specificity, are destroyed by periodate, whereas in S VI, the structures responsible for specificity are either less affected or yield derivatives with almost as much affinity for antibody as the original polysaccharide. S II and S VI are not adsorbed by washed rabbit or human erythrocytes, but their periodate-oxidized derivatives are readily taken up, presumably owing to their newly formed reactive aldehyde groups, giving high titers in passive hemagglutination. The method should be applicable to other polysaccharides which do not adsorb readily on red cells.

Animals↗
PubMed1961

Immunochemistry of pneumococcal types II, V, and VI. II. Inhibition tests in the type VI precipitating system.

Rebers, Paul A. (Rutgers University, New Brunswick, N. J.), Esther Hurwitz, and Michael Heidelberger. Immunochemistry of pneumococcal types II, V, and VI. II. Inhibition tests in the type VI precipitating system. J. Bacteriol. 82:920-926. 1961.-As in other immune systems involving polysaccharides, rabbit antibodies but not those engendered in the horse were found sensitive to degradation of type VI pneumococcal (Pn) polysaccharide (SVI), and were readily inhibited by fragments of SVI. Large amounts, 30 to 111 mumoles, of most sugars gave up to 15% inhibition, while sugar and polyol phosphates inhibited as much as 25%, with little relation to their presence or absence in SVI. The phosphate-free repeating unit of SVI was a good inhibitor, its phosphate monoester was better, and the "trimer" still better. The "trimer" precipitated most of the antibodies from horse anti-Pn VI.Although inhibition of precipitation of SVI anti-Pn horse sera could not be demonstrated with fragments of SVI, cross-reactions of antibodies in the horse sera could be inhibited. Precipitation of SII was inhibited by low concentrations of l-rhamnose, while even high concentrations of the other sugar components of SII and SVI were ineffective. Precipitation by guar gum was inhibited by galactose and alpha- and beta-methyl-galactopyranosides, also by rhamnose, although guar gum does not contain this sugar, while SVI, the antigenic determinant, does.

Animals↗
PubMed1960

The immunological specificity of type II Pneumococcus and its separation into partial specificities. II.

Quantitative data are given on the cross reactions in Type II antipneumococcal horse sera of plant gums and hemicelluloses containing multiple terminal groupings of glucuronic acid and/or 4-O-methylglucuronic acid. Great variability is shown both in the reactivities of the polysaccharides and in the antibodies in the sera of different animals immunized with the same antigen. The 4-O-methyl substituent on the glucuronic acid residues in a gum often appears to diminish cross-precipitation with antibodies to S II.

Animals↗