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M HEIDELBERGER

Publications and source records attributed to M HEIDELBERGER.

At least 37 records · Page 2Linked to original sources
PubMed1956

Cross-reactions of antityphoid and antiparatyphoid B horse sera with various polysaccharides.

A study was made of cross-reactions of synthetic polyglucose and of numerous plant and bacterial gums in an antityphoid and an antiparatyphoid B horse serum. The observed differences permit conclusions to be drawn regarding certain of the linkages likely to be found in the fine structures of each of the corresponding Salmonella polysaccharides:- 1. Cross-reactions of the antityphoid serum with the specific polysaccharide of Type II pneumococcus and with tamarind seed polysaccharide, glycogen and synthetic polyglucose indicate that the acetic acid-degraded O-polysaccharide of S. typhi, strain O 901, may contain part, at least, of its glucose as 1,4,6-branch points or in 1,6-linkage, perhaps adjacent to a terminal, non-reducing, galactopyranose unit. 2. Cross-reactions of both antisera with arabogalactans point to the existence of (probably beta-) 1,3-, 1,6-, and/or 1,3,6-linkages of galactose in both the typhoid and paratyphoid B polysaccharides. 3. The differential reactivities of the galactomannans and yeast mannan suggest that the mannose in the typhoid polysaccharide is linked 1,2- or 1,3- with possible non-reducing mannopyranose end groups attached 1,6-. In the paratyphoid B polysaccharide the linkages are probably galacto-oligomannose 1,4-, or 1,4,6-, or the corresponding linkages of mannose alone.

Cross Reactions↗
PubMed1956

The immunological specificity of type II Pneumococcus and its separation into partial specificities.

The specificity of Type II pneumococcus determined by its capsular polysaccharide (S II) may be separated into three partial specificities, each characteristic of one of the three component sugars of S II, namely, glucuronic acid, glucose, and rhamnose. By far the largest portion of antibodies in Type II antipneumococcus horse sera which cross-react with carbohydrates containing one or more of these sugars are reactive with those characterized by multiple groupings of glucuronic acid. This confirms, extends, and explains earlier observations. In confirmation of predictions based upon existing information tamarind seed polysaccharide (jellose), in which much of the glucose exists as 1,4,6-branch points, was found to react in Type II antisera. Several instances are given of cross-reactions in these antisera apparently due to the L-rhamnose component of the reacting polysaccharides. The antisera contain far more antibody capable of precipitating with substances with multiple units of glucuronic acid than with those so far tested containing multiple 1,4,6-branch points of glucose or multiple groupings of rhamnose. The long known increase of titer of gum arabic on partial hydrolysis is now fully explained and discussed, and a chemical picture is given of the change in "avidity." The sum of the partial specificities measured does not equal the whole. Quantitative data illustrating these points are given.

Cross Reactions↗
PubMed1954

Glycogen, an immunologically specific polysaccharide.

Glycogens from various animal and vegetable sources precipitate antipneumococcal horse sera of Types II, VII, IX, XII, XX, and XXII. Fractionated glycogen and glycogen recovered after reprecipitation, acetylation, and deacetylation precipitate the antisera, but glycogen degraded by saliva does not. A fraction of the antibody is precipitated in the antisera by glycogen. Possible chemical relationships accounting for these instances of cross-precipitation are discussed in terms of the structures of glycogen and the type-specific polysaccharides of pneumococcus and the quantitative theory of specific precipitation. Amylopectin also gives cross-reactions of smaller magnitude. Quantitative data on these are withheld until irregularities have been eliminated.

Animals↗
PubMed1952

Quantitative immunochemical studies with the purified factor in mouse milk connected with mammary carcinoma.

Antisera to the mouse milk factor (F), containing up to 0.5 mg. of antibody nitrogen per ml. are readily obtainable in rabbits, especially when adjuvants are added to the antigen. Quantitative data on the anti-F content of the sera are given, also on the total nitrogen precipitated by the non-casein fraction of C57 mouse milk which does not contain F. The antisera were also absorbed with casein fractions but the difficulties in the way of quantitative analysis were not overcome. Antisera to fractions of C57 mouse milk were also obtained and the reactions of F and the C57 proteins with these sera were studied. The results, as far as they go, are in accord with the earlier belief that the milk factor possesses an immunological specificity not present in normal mouse tissues.

Animals↗