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Biomedical subjects

M Kent

Publications and source records attributed to M Kent.

At least 19 recordsLinked to original sources
PubMed1992

Oligonucleotide labeling methods. 3. Direct labeling of oligonucleotides employing a novel, non-nucleosidic, 2-aminobutyl-1,3-propanediol backbone.

Novel CE-phosphoramidite (7a-e) and CPG (8a, c, d, e) reagents have been prepared from a unique 2-aminobutyl-1,3-propanediol backbone. The reagents have been used to directly label oligonucleotides with fluorescein, acridine, and biotin via automated DNA synthesis. The versatile 2-aminobutyl-1,3-propanediol backbone allows for labeling at any position (5', internal, and 3') during solid phase oligonucleotide synthesis. Multiple labels can be achieved by repetitive coupling cycles. Furthermore, the 3-carbon atom internucleotide phosphate distance is retained when inserted internally. Using this method, individual oligonucleotides possessing two and three different reporter molecules have been prepared.

Automation↗
PubMed1991

Intralobar pulmonary sequestration.

Intralobar pulmonary sequestration is a rare congenital anomaly, comprising a mass of non-functioning lung tissue, without normal bronchial and vascular connections. The condition has two distinct clinical presentations: 1. In the 1st year of life, it may present as the site of a significant arterio-venous shunt, and then is usually associated with other cardiac anomalies, becoming defined in the course of appropriate cardiovascular investigations. 2. In older children, the condition becomes manifest because of persistent radiological changes and inadequate response to treatment, following one or more bouts of respiratory infection. Occlusion of the shunt or removal of the sequestration is the definitive management; the importance of the condition relates to the need to consider the diagnosis in evaluating the clinical presentations indicated above.

Bronchopulmonary Sequestration↗
PubMed1990

Direct estimation of serological H-Y antigen by flow cytometry.

This study was undertaken to evaluate the efficiency of flow cytometry in the detection of the serological H-Y antigen, and to survey expression of H-Y in the normal human population. Peripheral blood leukocytes (granulocytes) were reacted with monoclonal H-Y antibody, gw-16, and with FITC-conjugated goat anti-mouse IgG, and then were scored for fluorescence in the flow cytometer. Assigned H-Y phenotype was correlated accurately with sex phenotype in 33 out of 38 women and 62 out of 65 men. Data were evaluated by exploratory data analysis (EDA).

Antibodies, Monoclonal↗
PubMed1988

DNA analysis by flow cytometry.

Accurate quantification of DNA from cells of several species is possible with flow cytometry. When one species is used as a reference, cytometric readings from two or more different species can be compared to obtain relative percent DNA or DNA indices. Differences in DNA from the male and female of the same species also can be measured. The method allows rapid screening of chromosomal abnormalities among large clinical populations, and evaluation of errors of sex determination such as XY sex reversal.

Animals↗
PubMed1988

Sex selection with monoclonal H-Y antibody.

H-Y antigen defined by antibody from male-sensitized female mice has been reported in male embryos of mouse, rat, cattle, goat, pig, and sheep. We now describe the use of monoclonal H-Y antibodies in identification of male and female during embryo transfer in cattle. Monoclonal H-Y antibodies were applied with fluorescein-isothiocyanate-conjugated goat antimouse immunoglobulin (Ig), and the embryos were scored for fluorescence under ultraviolet light. In alternative trials with 149 embryos, the method could be applied with 73% to 82% efficiency. Pregnancy rates for treated embryos were at least as high as those expected for untreated embryos. The calves that developed from the treated embryos are normal and growing.

Animals↗
PubMed1987

Enzyme immunoassay of H-Y antigen: experimental and clinical applications.

A newly developed enzyme-linked immunosorbent assay (ELISA) was applied for studying H-Y antigen in buffaloes, cattle, horses and humans. A monoclonal H-Y antibody was absorbed with cells from males or females and was then tested against fluid samples known to contain soluble H-Y antigen. In this system, positive absorption manifested itself by a fall in optical density relative to the optical density scored using unabsorbed antibody; this finding signified the presence of H-Y in the absorbing cells. In each of the four species, the fall in optical density was pronounced after absorption with male cells, but some decrease was also evident after absorption with female cells, indicating a degree of nonspecific attachment of the antibody.

Animals↗
PubMed1984

Esophageal anastomosis--an experimental model to study the anastomotic lumen and the influence of a transanastomotic tube.

The influence of an indwelling transanastomotic silicon tube on healing esophageal anastomosis in puppies was studied by making resin casts of the reconstructed esophagus. These casts demonstrated stenosis but no difference in the group with a transanastomotic tube was seen. Stenotic index of the puppies varied from 0.7 to 0.97 (no stenosis = 1). An important observation made during the study was the presence of shelfing of scar tissue on the posterior esophageal wall. This was the site of mucosal ulceration, granuloma formation, and fibrous scarring where interrupted 0000 silk knots had been tied intraluminally. The anterior wall had a linear scar, where the knots were tied extraluminally. Extraluminal knots would be part of the technique to achieve a minimal amount of scar tissue at the site of the esophageal anastomosis.

Animals↗
PubMed1983

The treatment of Wilms' tumor: results from Royal Children's Hospital, Melbourne, 1967-1977.

A retrospective review of the treatment of Wilms' tumor from 1967 to 1977 at the Royal Children's Hospital, Melbourne, was undertaken, allowing a minimum 2-yr followup. Fifty-four (77%) of the 70 patients in the study are alive, with 23/25 (92%) in Stage I, 12/16 (75%) in Stage II, 12/19 (63%) in Stage III, and 7/9 (78%) in Stage IV. Survival was significantly better in Stage I disease, and those patients less than 5-yr-old at presentation. Recurrence of disease was significantly lower after the introduction of multiple courses of chemotherapy in 1971. Sixteen deaths occurred during the period: three infants from drug toxicity, four patients with "unfavorable" sarcomatous histology, and four patients with recurrent local disease, where irradiation had been confined to the tumor bed after tumor spill with recurrence beyond the irradiated field.

Abnormalities, Multiple↗
PubMed1978

Pulmonary interstitial emphysema requiring lobectomy. Complications of assisted ventilation.

An infant with hyaline membrane disease treated with intermittent positive pressure ventilation developed pulmonary interstitial emphysema localised to one lobe after collapse of the affected lobe. The development of tension and further symptoms necessitated lobectomy, after which the infant became totally asymptomatic. Microscopy of the resected lobe showed the unusual feature of giant cells lining the air-containing cysts. The presence of these multinucleate cells suggested the cysts may have represented greatly dilated lymphatic channels resulting from rupture of gases into the pulmonary lymphatics.

Humans↗