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M Kowalska

Publications and source records attributed to M Kowalska.

At least 73 records · Page 4Linked to original sources
PubMed1980

Bactericidal properties of lysosomal proteins obtained from human polymorphonuclear leucocytes (PMNL) examined in vitro. I. Characteristics of obtained fractions of lysosomal proteins.

In the present investigation the fractions obtained from lysosomal proteins of human peripheral blood granulocytes were analysed morphologically. In the analysis a column with DEAE-cellulose and ultracentrifugation in stepwise saccharose were employed. Electro-microscopic examinations of every fraction of lysosomes pointed to their morphological differences; the highest contents of lysosomal bags was noted in fraction number III. Correlations between activity of "indicatory" enzymes and content of protein and bactericidal activity in four studied fractions were discovered.

Acid Phosphatase↗
PubMed1980

Bactericidal properties of lysosomal proteins obtained from human polymorphonuclear leucocytes (PMNL) examined in vitro. II. Morphological estimation of cells treated with lysosomal proteins.

The present paper is concerned with the action of lysosomal proteins isolated from granulocytes of human peripheral blood on Gram-plus and Gram-minus bacteria. Differences in bacterial sensitivity to these proteins were found. The highest bactericidal activity was observed while bacteria from Staphylococcus and Escherichia were treated with lysosomal proteins. No structural changes in cells of bacteria from Streptococcus and Mycobacterium tuberculosis were observed. The sensitive bacteria cell walls underwent destruction mainly but changes also concerned cytoplasma which was destroyed.

Bacteria↗