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Biomedical subjects

M L Ceccuzzi

Publications and source records attributed to M L Ceccuzzi.

5 recordsLinked to original sources
PubMed1980

[Method for testing niacin bound activities in man].

In order to value the availability of niacin in man, it has been more suitable to test erythrocyte nicotinate-phosphoribosyltransferase and nicotinamide-phosphoribosyltransferase activities. These enzymes catalyze the production of Des-NMN and NMN from free bases. The radioactive compounds, produced from [14C] NAc and [14C] NAm, are isolated by a gel chromatography and counted in liquid scintillation. It has been observed that nicotinamide-phoshoribosyltransferase is scarcely active in human erythrocytes, while the nicotinate-phosphoribosyltransferase gives a good indication of niacin availability.

Carbon Radioisotopes↗
PubMedDate not supplied

Characterization of NAD-glycohydrolase in rat lymphoid organs.

Rat thymus and thymocytes possess an NAD-glycohydrolase activity lower than spleen and spleen lymphocytes. In all cases, the highest activity (50%) is bound to the nuclei, while that in the soluble fraction is higher (in percent) in the lymphocytes than in the whole organ. Considering that thymus and thymocytes are characterized by a soluble NAD-ase activity higher than in spleen and in spleen lymphocytes, and that lymphocytes in general have a greater soluble than microsomal activity, we interpret these findings are evidence that the soluble NAD-ase is due essentially to T lymphocytes, of which it represents a typical feature.

Animals↗