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PubMed · 7006633

[Method for testing niacin bound activities in man].

S Sestini·M L Ceccuzzi·C Ricci
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Abstract

In order to value the availability of niacin in man, it has been more suitable to test erythrocyte nicotinate-phosphoribosyltransferase and nicotinamide-phosphoribosyltransferase activities. These enzymes catalyze the production of Des-NMN and NMN from free bases. The radioactive compounds, produced from [14C] NAc and [14C] NAm, are isolated by a gel chromatography and counted in liquid scintillation. It has been observed that nicotinamide-phoshoribosyltransferase is scarcely active in human erythrocytes, while the nicotinate-phosphoribosyltransferase gives a good indication of niacin availability.

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Carbon RadioisotopesErythrocytesHumansKineticsNicotinamide PhosphoribosyltransferaseNicotinic AcidsPentosyltransferasesRadioisotope Dilution TechniqueEnglish AbstractJournal Article

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S Sestini, M L Ceccuzzi, C Ricci. 1980-06-30. [Method for testing niacin bound activities in man].. https://pubmed.ncbi.nlm.nih.gov/7006633/

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