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Biomedical subjects

M L Kaeberle

Publications and source records attributed to M L Kaeberle.

47 records · Page 3Linked to original sources
PubMed1980

Chemiluminescence by Listeria monocytogenes.

Listeria monocytogenes cells suspended in brain heart infusion broth or in carbonated saline solution emitted light (chemiluminescence) that could be detected by a liquid scintillation spectrometer. This chemiluminescence was inhibited by superoxide dismutase and catalase but not by the hydroxyl radical scavengers mannitol and benzoate; it was also dependent upon and proportional to the carbonate ion concentration in the medium. Organisms suspended in carbonated saline solution which had ceased to chemiluminesce immediately began to chemiluminesce again when acetaldehyde was added but not when glucose, sucrose, or xanthine was added. Acetaldehyde-induced chemiluminescence was inhibited by suproxide dismutase and catalase but not by allopurinol. Our data indicate that the superoxide anion, hydrogen peroxide, and the carbonate ion are involved in chemiluminescence by L. monocytogenes. Chemiluminescence is apparently initiated by the extracellular generation of superoxide anon by this organism. The mechanism for the production of the superoxide anion is not known, but xanthine oxidase does not appear to be involved.

Carbonates↗
PubMed1980

Increased susceptibility of lead-exposed swine to Salmonella choleraesuis var kunzendorf.

The effect of oral subclinical lead exposure on the susceptibility of swine to Salmonella choleraesuis var kunzendorf was studied in two experiments. In experiment 1, 18 pigs were exposed to lead acetate and 18 pigs were exposed to sodium acetate for 1, 2, or 3 weeks and then challenge exposed intraperitoneally. In experiment 2, 8 pigs were exposed to lead chloride for 2 weeks, 8 pigs were exposed to lead chloride for 1 week, and 8 pigs were exposed to sodium chloride for 2 weeks. These pigs were then challenge exposed orally. Only one pig (sodium acetate-exposed) died during experiment 1, but clinical signs were more severe in sodium acetate-exposed than in lead acetate-exposed animals. In experiment 2, 93.6% of the lead-exposed pigs died, whereas control pigs had 50.0% mortality. In experiment 2, clinical signs were more severe in lead-exposed pigs, and bacteria were more widespread in the tissues of these animals. Significant reductions of beta- or gamma-globulin concentrations or of agglutinating antibody titers were not observed in lead-exposed pigs in either of the trials. Possible mechanisms of the increased susceptibility in lead-exposed pigs of experiment 2 are discussed.

Animals↗
PubMed1980

Thymic abnormalities and growth hormone deficiency in dogs.

A high frequency of occurrence of a wasting disease, unthriftiness, and retarded growth was observed in a group of inbred Weimaraner dogs. Affected pups had a small thymus gland, with a marked absence of thymic cortex. A litter of eight pups from a sire and dam that were known to have produced affected offspring was chosen for further study. The pups had normal concentrations of WBC and gamma-globulins and were able to produce antibody in response to Brucella abortus. Two pups in the litter developed a wasting syndrome and responded well to therapy with thymosin fraction 5. One pup that survived the wasting syndrome had a significant (P < 0.05) depression of its lymphocyte blastogenic response to phytohemagglutinin compared with its surviving littermates. Pups from this litter also lacked a normal increase in plasma growth hormone concentration after the injection of clonidine HCl. These pups had concurrent abnormalities of the thymus-dependent immune function and in growth hormone metabolism. The syndrome in these pups has some features in common with the syndrome in the Ames or Snell-Bagg strains of immunodeficient dwarf mice.

Animals↗
PubMed1978

Microtitration agglutination for detection of Treponema hyodysenteriae antibody.

A microtitration agglutination test for the detection of Treponema hyodysenteriae antibody in swine and rabbit sera is described. The following methods provided the best test results: antigen produced from the spirochete after a culturing period of 36 to 44 h at 38 degrees C, washed antigen inactivated with 0.01% Merthiolate at 4 degrees C for 24 to 36 h, sera heated at 56 degrees C for 30 min, a diluent of phosphate-buffered saline (0.01 M, pH 7.2), and test results read macroscopically after 18 to 24 h of incubation at 38 degrees C. The test enabled detection of antibody against pathogenic T. hyodysenteriae with a high level of consistency and sensitivity. Sera against nonpathogenic T. hyodysenteriae produced low agglutinating titers (less than or equal to 1:8) when reacted against antigen from pathogenic isolates. Inactivated antigen remained stable for 7 to 10 days. Specificity of the reaction in the agglutination test was shown by absorption studies.

Agglutination Tests↗
PubMed1975

Immune response and the B blood group locus in chickens.

Chickens of three blood group genotypes, B1B1, B1B2 and B1B19, were compared in their ability to produce antibodies. The B locus is the major histocompatibility locus in this species. Homozygous B1 pullets had significantly higher adult mortality than did the B1 heterozygotes. In two separate experiments the B1B1 females produced significantly fewer antibodies to Salmonella pullorum than did the B1 heterozygotes. Also the B1B1 pullets responded with lower antibody titers following immunization with ferritin, bovine serum albumin and parainfluenza-3 virus, although the differences were not significant. The results of this study suggest that an immune response gene is associated with the major histocompatibility locus in chickens, paralleling the H-2 locus and Ir genes in mice.

Alleles↗
PubMed1971

Lead suppression of mouse resistance to Salmonella typhimurium.

Mice were treated with subclinical doses of lead nitrate for 30 days. Lead-treated mice showed greater susceptibility to challenge with Salmonella typhimurium than controls which received no lead. This result confirms the hypothesis that treatment with lead reduces the resistance of mice to bacterial infection.

Animals↗