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Biomedical subjects

M M Hussein

Publications and source records attributed to M M Hussein.

At least 37 records · Page 2Linked to original sources

Studies on chronic liver diseases in patients with and without Toxoplasma latent infection.

Chronic liver diseases mainly due to schistosomiasis is a common clinical feature in Egypt. On the other hand, Toxoplasma latent infection is another common feature. In this paper, it was aimed to study the role of Toxoplasma latent infection in patients with chronic liver diseases mainly due to schistosomiasis. The latent Toxoplasma infection was measured by the positivity of IgG to ELISA and the negativity of IgM to DS-ELISA. The cellular immunity was assessed by the dinitrochlorobenzene (DNCB) skin test. The clinical and the laboratory studies showed that latent Toxoplasma infection had no marked effect on the progression of the chronic liver diseases.

Chronic Disease↗

Plasma fibronectin concentrations in bleeder and non bleeder patients with hepatosplenic schistosomiasis.

This study was conducted on 40 patients with schistosomal hepatosplenomegaly and oesophageal varices "O.V.". They were divided into two groups: Group A were suffering from bleeding O.V and group B were non bleeders. Ten Healthy individuals were also included in the study as control group. Plasma fibronectin concentration was estimated in each subject using quantitative single radial immuno-diffusion technique. The results of the study showed that plasma fibronectin concentrations were significantly decreased in both groups of patients, bleeders (354.5 Mg/dl +/- 109.2) and non bleeders (307 +/- 103.5) compared to the control group (514 +/- 57.9). However, there was no significant difference in fibronectin concentration between group A and group B (p greater than 0.05). There was insignificant correlation between plasma fibronectin and some haemostatic estimations (clotting time, bleeding time and platelet count) in the studied groups (p greater than 0.05). Also, there was insignificant difference in fibronectin concentration in patients with (312 Mg +/- 116.4) and without (368.6 +/- 106.9) liver cell failure.

Adolescent↗

Antiparietal cell antibodies in gastritis associated with chronic liver diseases.

Thirty patients with gastritis associated with chronic liver disease and ten normal controls were the subject of this study. Pathologically about 18 were diagnosed as chronic atrophic gastritis, 10 as chronic superficial gastritis, 1 as gastric atrophy and 1 as hypertrophic gastritis. Parietal cell antibodies (PCA) were detected (IFA) in 2 cases with no significant relation.

Autoantibodies↗

A general survey of blood and tissue parasites of some rodents in Arbil province, Iraq.

In a survey of blood and tissue parasites from 233 mice and rats, consisting of 105 specimens of house mice (Mus musculus), 99 black rats (Rattus rattus), and 29 Norway rats (Rattus norvegicus), collected from different localities of Arbil province, northern Iraq, Trypanosoma lewisi was found in 7 (7.07%) of 99 R. rattus and in 5 (17.86%) of 29 R. norvegicus, while Trypanosoma musculi was found in 4 (3.80%) of 105 M. musculus and this is the first record of this trypanosome from rodents in Iraq. During the preparation of tissue impression smears we came across 2 specimens of Fasciola hepatica in the livers of 2 R. norvegicus and the incidence of infection was 6.89%. Examination of other organs revealed no parasites. F. hepatica which is recorded, in the present study, for the first time from rodents in Iraq, is of particular importance since it infects both humans and domestic animals.

Animals↗

Severe hyponatraemia in burn patients secondary to hydrotherapy.

Severe hyponatraemia, hyperkalaemia and pre-renal uraemia were seen in two burn victims who were treated with repeated submersion in tap-water. A primary loss of salt through the burned surface area into the whirlpool bathwater has been documented in both cases. All these abnormalities have been partially corrected by adding salt to the whirlpool water. To our knowledge no similar documented cases exist in the literature.

Adolescent↗

Large-bore coated columns for sampling and concentration of organic volatiles in air, headspace and water analysis.

Large-bore coated (LBC) columns were used as sampling and concentrating traps in analyses for traces of organic volatiles in air and water. This simple technique utilizes long metal columns thinly coated with SE-30 for direct trapping of the organics. The sample is simply passed through the LBC column; the trapped organics are then thermally desorbed onto a conventional porous polymer pre-column or onto a second LBC column. If desired, this can be shorter or narrower bore than the initial LBC sampling column. The sample is finally desorbed onto the gas chromatographic column for analysis. Multiple transfers between LBC columns are possible, with increased concentration at each transfer, resulting in a "concentration pump" effect. The technique offers the advantages of great simplicity, efficiency and ease of sample transfer. Samples are obtained with low back-pressure and minimal interfering artifacts. The system shows almost complete imperturbability to moisture. Indifference to moisture and the low back-pressure enable direct sampling of very large volumes of air and even breath. Direct sampling of aqueous systems was also possible. The latter area was not fully investigated but offers potential for water pollution analysis and in direct examination of biological fluids and aqueous flavor extracts where heat sensitivity is a problem. With LBC columns the sampling and concentration sequence exposes the substances sought to no more drastic conditions than those they will be subjected to in the process of gas chromatographic analysis.

Air↗

Prevention of early recurrence of spontaneous subarachnoid haemorrhage by epsilon-aminocaproic acid.

A controlled clinical trial of epsilon-aminocaproic acid (E.A.C.A.), 36 g/day, was undertaken to assess its effectiveness in reducing immediate recurrence in patients with spontaneous subarachnoid haemorrhage (S.A.H.) proved by lumbar puncture. Of 83 patients treated with E.A.C.A., 3 (4%) had recurrent haemorrhage, and 1 (33%) of these died. Of 82 control patients who were not given any antifibrinolytic drug, 22 (26%) had recurrent haemorrhage, and 10 (45%) of these patients died. E.A.C.A. produced a striking reduction in the early recurrence of S.A.H. No serious side-effect resulted.

Adolescent↗

Single sample estimates of renal clearances.

Thirty adult patients were investigated with 36 125I Hippuran and 40 51Cr EDTA single injection clearance studies. "Volumes of distribution" obtained from single samples have been correlated with the slope clearances and regression equations have been determined. From these equations it is possible to determine glomerular filtration rate (GFR) and effective renal plasma flow (ERPF) from single plasma samples and the method is sufficiently accurate for routine application.

Adult↗

Monotherapy with labetalol for hypertensive patients with normal and impaired renal function.

1 Labetalol was given to 41 hypertensive patients in a divided dosage of 150--2,400 mg daily for periods ranging from 1--64 months. 2 Monotherapy with labetalol was adequate in 12 out of 19 patients with essential hypertension and in 15 out of the 22 with renal hypertension. 3 Following a single dose of labetalol 200 mg orally a hypotensive response was seen between 1.5 and 2 hours. 4 In the doses used there was no exercise or postural hypotension. 5 No reduction in overall renal function attributable to labetalol was seen.

Blood Pressure↗

Utilization of kerosene by a hydrocarbon-assimilating strain of Fusarium oxisporum.

Fusarium oxysporum IFO 6384 was grown in three different media, one containing glucose and two containing kerosene as carbon source. One of the kerosene media contained also Tween 60. The harvested dry mats were weighed at various stages of growth. Substitution of glucose with kerosene led to a sharp drop in the production of fungal mycelia. Analysis of the mat samples representing maximum yields indicated that the mycelia grown in the kerosene media were richer in free amino acid and poorer in protein as well as in polymeric and low-molecular weight carbohydrates than those grown in the glucose medium. Treatment of the culture filtrates with three volumes of acetone led to the separation of two fractions. In the first, acetone soluble fraction the free sugars and amino acids were determined, whereas the second, acetone precipitable one, was examined for its proteolytic activity. The gelatinase activity of the acetone precipitable material (APM) obtained from the kerosene media was lower than that from the glucose medium. The former activity, in contrast to the latter reached its maximum and minimum values at pHs 4 and 9, respectively.

Cell Division↗

Carbohydrates from hydrocarbons. II. Free and bound sugars from yeast cells grown on n-hexadecane.

Candida lipolytica (strain 10) was grown on an n-hexadecane medium with and without yeast extract. The harvested dry cells were weighed at various stages of growth. The free sugars from the cultures were obtained by Soxhlet extraction with 85% ethyl alcohol. Further qualitative and quantitative analyses of free monosaccharides in the concentrated alcoholic extracts were made by paper chromatography. Glucose was the only free monosaccharide that could be identified at various stages of growth. The chromatographic analysis of the acid-hydrolyzed yeast cells indicated the presence of glucose and mannose as dominant bound sugars; galactose and xylose were present in minor quantities. In the harvested dry cells from the yeast extract-containing medium, in general, greater amounts of bound sugars were present.

Alkanes↗

The production of extracellular polysaccharides by a hydrocarbon assimilating yeast.

Investigations were made on the extracellular polysaccharides production by a hydrocarbon assimilating yeast. The yeast Candida lipolytica was grown on two different media containing n-hexadecane as the sole carbon source. Polymeric materials precipitated from the culture medium with ethanol were determined gravimetrically at various growth periods. The hydrolysis of the precipitated material and their chromatographic analysis revealed the presence of mannose, glucose and galactose in the yeast extract-containing medium. The proportions of these sugars differed at various growth periods. The hydrolysates of the polymers of the yeast extract-free medium contained more xylose than those of the yeast extract containing medium.

Candida↗