[Cytologic study of the peritoneal fluid by puncture of Douglas' pouch].
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Biomedical subjects
Publications and source records attributed to M Maillet.
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We are reporting here the results of an electron microscopic study of the junction between cells which have been seen in corpora lutea in human beings between the 20th and 27th days of the cycle. Conventional electron microscopic techniques, using tracers (plotters) for the extracellular spaces as well as Thiery's histochemical technique, were used. Two principal types of junctions between the cells have been described: tight junctions and septate junctions. A discussion takes place about the different histophysiological problems which presented (LH hormone receptors and intercellular diffusion of the message).
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Three different types of junctions were revealed studying human corpus luteum during luteotrophic phasis : tight-junctions, gap-junctions and septate junctions. Tight-junctions were characterized by a discontinuous central leaflet formed by osmiophilous punctiform components separated by osmiophobic spaces. Lanthanum nitrate permeability of these junctions lead us to think that they may be leaky. They were only a very few gap-junctions, they were found to be as classically described, made up of seven leaflets. Septate junctions were about 250 A long, some septa were arrayed in a discordely fashion and were separated by lanthanum nitrate permeable spaces. The location of these different junctions was determined with accuracy: tight and gap junctions were located on the lateral faces and around cellular interdigitations, septate junctions were shown to bind either the microvillosities of a same cell or the microvillosities of two bordering cells. The fonctionnal signification of these junctions is discussed. Les auteurs remercient vivement M. le Professeur SOUTOUL et M. le Docteur LANSAC du Service de Gynécologie-Obstétrique (C.H.U. Bretonneau) pour leur aimable collaboration.
Female Albino Rats exposed to permanent light, have polycystic and anovulatory ovaries, with a vaginal cytology composed of cornified cells (persistent estrus) and a plasmatic hyperandrogenia. These structural modifications are similar to those of women affected by Stein-Leventhal's syndrome. The possible role of regulation of the pineal gland is considered.
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